AP09429C5-N Biotin F(ab')2 antibody

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Goat anti Biotin F(ab')2


Product Description for Biotin F(ab')2

Goat anti Biotin F(ab')2.
Presentation: Cy5
Product is tested for Enzyme Immunoassay, Western blot / Immunoblot.

Properties for Biotin F(ab')2

Product Category Primary Antibodies
Quantity 1 mg
Presentation Cy5
Applications E, WB
Clonality Polyclonal
Host Goat
Isotype IgG
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Biotin conjugated to Keyhole Limpet Hemocyanin (KLH)
Application This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Recommended Dilutions:
IF Microscopy: 1/100-1/1,000.
Flow Cytometry: 1/200-1/400.
Background Biotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da.
Concentration 1.0 mg/ml (by UV absorbance at 280 nm)
Storage Prior to reconstitution store at 2-8°C.
Following reconstitution store the antibody undiluted at 2-8°C for one month
or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
29 (Cyanine 5.29-OSu) (Molecular Weight 975 daltons)
Absorption emission:
650 nm / 667 nm
Molar radio:
5.3 moles Cy5 per mole of Goat IgG F(ab’)2
Immunoaffinity Chromatography
Buffer System:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 10 mg/ml BSA (Immunoglobulin and Protease free) as stabilizer and 0.01% (w/v) Sodium Azide as preservative
Lyophilized F(ab')2 fragment
Restore with 1.0 ml of deionized water (or equivalent).
For extended storage, add glycerol to 50%
This is a Cy5 conjugated F(ab')2 fragment of Anti-Biotin.

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