AP09093SU-N Biotin Ligase Tag / BLT antibody

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0.1 ml / €420.00
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Rabbit anti Escherichia coli Biotin Ligase Tag / BLT

Product Description for Biotin Ligase Tag / BLT

Rabbit anti Escherichia coli Biotin Ligase Tag / BLT.
Presentation: Serum
Product is tested for Enzyme Immunoassay, Western blot / Immunoblot.

Properties for Biotin Ligase Tag / BLT

Product Category Primary Antibodies
Quantity 0.1 ml
Synonyms Biotin Ligase Epitoe Tag, Biotin-[acetyl-CoA-carboxylase] synthetase, BirA, bioR, dhbB
Presentation Serum
Reactivity E. coli
Applications E, WB
Clonality Polyclonal
Host Rabbit
Isotype IgG
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
GLNDIFEAQKIEWH (Minimal BirA substrate; Biotin is added to the sequence at the Lysine)
Application ELISA: 1/5,000.
Western blot: 1/5,000.
Background In Escherichia coli, transfer of biotin to the epsilon amino group of a specific lysine residue of the biotin carboxyl carrier protein (BCCP) is accomplished by the biotin holoenzyme synthetase BirA. The minimal substrate requirement of BirA-catalyzed biotinylation is a 14-residue peptide known as the biotin ligase tag (BLT). This antibody can be used to detect BLT in recombinant expression systems.
Concentration 70 mg/ml (by Refractometry)
General Readings
  1. Schatz PJ. Use of peptide libraries to map the substrate specificity of a peptide-modifying enzyme: a 13 residue consensus peptide specifies biotinylation in Escherichia coli. Biotechnology (N Y). 1993 Oct;11(10):1138-43. PubMed PMID: 7764094.
  2. Beckett D, Kovaleva E, Schatz PJ. A minimal peptide substrate in biotin holoenzyme synthetase-catalyzed biotinylation. Protein Sci. 1999 Apr;8(4):921-9. PubMed PMID: 10211839. (Free PMC Article available)
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Buffer System:
0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2
Preservatives:
0.01% (w/v) Sodium Azide
Stabilizers:
None
State:
Liquid (sterile filtered) Serum
Serum
Specificity
Specificity:
This antiserum detects the minimal peptide substrate (GLNDIFEAQKIEWH) for the E. coli Biotin ligase tag (BLT of BirA). This peptide is also called the Biotin Acceptor Peptide (BAP) of the Biotin Carboxyl Carrier Protein (BCCP) subunit of acetyl CoA carboxylase of E. coli.
Western blot analysis of B. subtilis strains producing either (BAP-tagged) SPoIIIAH (XWB49) or (BAP-tagged) SpoIIQ (XWB46) showed strong and specific reactivity to the antisera. Minor background bands of unknown identity may be observed. The antisera will recognize the BAP/BLT tag irrespective of the whether the tag is biotinylated (data not shown).
The peptide is a substrate for Biotin ligase, which places a Biotin moiety on the central lysine of the peptide when it interacts with BirA (the Biotin ligase). When proteins contain this sequence tag, they can be very efficiently pulled down after biotinylation using streptavidin beads.

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