AP07900PU-N BLM (1319-1335) antibody

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50 µg / €380.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

Quick Overview

Rabbit anti Human BLM

Product Description for BLM

Rabbit anti Human BLM.
Properties: (1319-1335)
Presentation: Purified
Product is tested for Enzyme Immunoassay, Paraffin Sections.

Properties for BLM

Product Category Primary Antibodies
Target Category
Quantity 50 µg
Synonyms Bloom syndrome protein, DNA helicase, RECQ2, RECQL3, RecQ protein-like 3, RecQ-like type 2
Presentation Purified
Reactivity Hu
Applications E, P
Clonality Polyclonal
Host Rabbit
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P54132
Immunogen:
Synthetic peptide from human BLM, aa 1319-1335
GeneID:
641
Property 1319-1335
Application ELISA: 1/1000.
Immunohistochemistry on Paraffin Sections: 10 µg/ml.
Background The Bloom's syndrome (BS) gene, BLM, plays an important role in the maintenance of genomic stability in somatic cells. The BLM protein is a 1417 amino acid peptide with homology to the RecQ helicases, a subfamily of DExH box-containing DNA and RNA helicases. The BLM protein has similarity to 2 other proteins that are members of the subfamily, namely the gene product encoded by RECQL2, also called the Werner syndrome gene (WRN), and the product of the yeast gene SGS1. These proteins may interact with topoisomerases, have 42 to 44% amino acid identity across the conserved helicase motifs, are of similar length and contain highly negatively charged N-terminal regions and highly positively charged C-terminal regions.
The BLM protein is located in the nucleus of normal human cells in the nuclear domain 10 (ND10) or promyelocytic leukemia nuclear (PML) bodies. These structures are punctate deposits of proteins disrupted upon viral infection and in certain human malignancies. BLM was found primarily in ND10 except during S phase, when it colocalized with the Werner syndrome gene product, WRN, in the nucleolus. The BLM protein is likely to be part of a DNA surveillance mechanism operating during S phase - BLM was found to be part of the BASC (BRCA1-associated genome surveillance) complex, which may serve as a sensor of abnormal DNA structures and/or as a regulator of the postreplication repair process.
Bloom syndrome cells show marked genomic instability; in particular, hyperrecombination between sister chromatids and homologous chromosomes - SCE (sister chromatid exchanges). In vitro BLM selectively binds Holliday junctions formed during genetic recombination and acts on recombination intermediates containing a Holliday junction to promote ATP-dependent branch migration. BLM may disrupt potentially recombinogenic molecules that arise at sites of stalled replication forks.
Concentration 1 mg/ml
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Dilute only prior to immediate use.
Avoid cycles of freezing and thawing.
Shelf life: One year from despatch.
Format
Purification:
Protein G Chromatography
Buffer System:
Phosphate Buffered Saline PBS containing 0.09% Sodium Azide as preservative
State:
Liquid purified IgG fraction
Purified
Species Reactivity
Species reactivity (tested):
Human
Specificity
Specificity:
Recognises Bloom's Syndrome Protein (BLM) at aa 1319-1335.
Gene ID 641

Accessory Products

Proteins and/or Positive Controls

Proteins for BLM (2 products)

Catalog No. Species Pres. Purity   Source  

BLM

BLM Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

BLM

BLM Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.
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