AP55713PU-N BLM pThr99 antibody

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0.1 mg / €340.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

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Rabbit anti Human BLM

AP55713PU-N

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  • AP55713PU-N

Product Description for BLM

Rabbit anti Human BLM.
Properties: pThr99
Presentation: Aff - Purified
Product is tested for Paraffin Sections, Western blot / Immunoblot.

Properties for BLM

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms Bloom syndrome protein, DNA helicase, RECQ2, RECQL3, RecQ protein-like 3, RecQ-like type 2
Presentation Aff - Purified
Reactivity Hu
Applications P, WB
Clonality Polyclonal
Host Rabbit
Molecular weight 159 kDa
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P54132
Immunogen:
Peptide sequence around phosphorylation site of threonine 99 (Q-E-T(p)-Q-R) derived from Human Bloom Syndrome (KLH-conjugated)
GeneID:
641
Property pThr99
Application Western blot: 1:500~1:1000.
Immunohistochemistry on paraffin sections: 1:50~1:100.
Background Participates in DNA replication and repair. Exhibits a magnesium-dependent ATP-dependent DNA-helicase activity that unwinds single- and double-stranded DNA in a 3'-5' direction. Involved in 5'-end resection of DNA during double-strand break (DSB) repair: unwinds DNA and recruits DNA2 which mediates the cleavage of 5'-ssDNA. Negatively regulates sister chromatid exchange (SCE).
Concentration 1.0 mg/ml
General Readings
  1. Ellis NA, Groden J, Ye TZ, Straughen J, Lennon DJ, Ciocci S, et al. The Bloom's syndrome gene product is homologous to RecQ helicases. Cell. 1995 Nov 17;83(4):655-66. PubMed PMID: 7585968.
  2. Karow JK, Chakraverty RK, Hickson ID. The Bloom's syndrome gene product is a 3'-5' DNA helicase. J Biol Chem. 1997 Dec 5;272(49):30611-4. PubMed PMID: 9388193.
  3. Pichierri P, Franchitto A, Rosselli F. BLM and the FANC proteins collaborate in a common pathway in response to stalled replication forks. EMBO J. 2004 Aug 4;23(15):3154-63. Epub 2004 Jul 15. PubMed PMID: 15257300. (Free PMC Article available, 7 images available)
Storage Upon receipt, store undiluted (in aliquots) at -20°C.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Affinity chromatography using epitope-specific peptide
Buffer System:
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
State:
Liquid Ig fraction
Aff - Purified
Species Reactivity
Species reactivity (tested):
Human
Specificity
Specificity:
The antibody detects endogenous levels of Bloom Syndrome Protein only when phosphorylated at threonine 99.

Accessory Products

Proteins and/or Positive Controls

Proteins for BLM (2 products)

Catalog No. Species Pres. Purity   Source  

BLM

BLM Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

BLM

BLM Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.
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