TA306104 BMF (N-term) antibody

Rabbit Polyclonal Bmf Antibody

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0.1 mg / €415.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

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Rabbit anti Human, Mouse BMF

TA306104

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  • TA306104

Product Description for BMF

Rabbit anti Human, Mouse BMF.
Properties: (N-term)
Presentation: Aff - Purified
Product is tested for Enzyme Immunoassay, Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot.

Properties for BMF

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms Bcl-2-modifying factor
Presentation Aff - Purified
Reactivity Hu, Ms
Applications E, ICC/IF, WB
Clonality Polyclonal
Host Rabbit
Isotype IgG
Shipping to Europe, USA/Canada
PDF datasheet View Datasheet
Manufacturer OriGene Technologies, Inc.

Datasheet Extract

Immunogen
Swiss Prot Num:
Q96LC9
Immunogen:
Human Bmf (N-Terminus) Peptide (AP30157CP-N)
GeneID:
90427
Property N-term
Add. information Blocking peptide available: blocking peptide (AP30157CP-N)
Application ELISA.
Western Blot: Bmf antibody can be used for detection of Bmf at 2 μg/ml. HepG2 or 293 cell lysate can be used as a positive control and a band at approximately 25 kDa can be detected.
Immunocytochemistry.
Background Apoptosis is related to many diseases and development. Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2 homology 3 (BH3) domain is a potent death domain. BH3-only proteins, including Bad, Bid, Bik, Hrk, Bim, Noxa, and PUMA, form a growing subclass of the Bcl-2 family. A novel BH3-only protein was recently identified in human and mouse and designated Bmf (for Bcl-2-modifing factor) (1). The BH3 domain in Bmf is required both for binding to Bcl-2 proteins and for triggering apoptosis. In healthy cells, Bmf associates with the dynein light chain 2 (DLC2) component of the myosin V motors and is sequestered by the cell's actin cytoskeleton. Disruption of the actin cytoskeleton, either by depolymerization of actin filaments or by detachment of cells from the extracellular matrix, triggers release and activation of Bmf, initiating the downstream apoptotic program (1,2). Bmf is constitutively expressed in many tissues (1,2).
General Readings
  1. Puthalakath H, Villunger A, O'Reilly LA, Beaumont JG, Coultas L, Cheney RE, et al. Bmf: a proapoptotic BH3-only protein regulated by interaction with the myosin V actin motor complex, activated by anoikis. Science. 2001 Sep 7;293(5536):1829-32. PubMed PMID: 11546872.
  2. Hunt A, Evan G. Apoptosis. Till death us do part. Science. 2001 Sep 7;293(5536):1784-5. PubMed PMID: 11546862.
Storage Store the antibody undiluted at 2-8°C.
Shelf life: one year from despatch.
Format
Purification:
Affinity chromatography purified via peptide column
Buffer System:
PBS containing 0.02% sodium azide.
State:
Liquid purified Ig fraction
Aff - Purified
Specificity
Specificity:
EndoG antibody was raised with a synthetic peptide corresponding to 15 amino acids near the amino terminus of human EndoG.
Species:
Human, Mouse.

Accessory Products

Proteins and/or Positive Controls

Proteins for BMF (7 products)

Catalog No. Species Pres. Purity   Source  

BMF (transcript variant 2)

BMF Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €748.00
  OriGene Technologies, Inc.

BMF (full length, N-term HIS tag)

BMF Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
50 µg / €205.00
  OriGene Technologies, Inc.

BMF (1-129, T7-tag)

BMF Human Purified > 90 % by SDS - PAGE E. coli
0.5 mg / €1,070.00
  OriGene Technologies GmbH

BMF (1-129, T7-tag)

BMF Human Purified > 90 % by SDS - PAGE E. coli
0.1 mg / €400.00
  OriGene Technologies GmbH

BMF

BMF Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

BMF

BMF Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

BMF

SDS-Page: Bmf Protein [NBC1-28000] - BMF, 15.6 kDa (144aa), confirmed by MALDI-TOF with a purity of 90% by SDS - PAGE Protein
  Novus Biologicals Inc.

Positive controls for BMF (2 products)

Catalog No. Species Pres. Purity   Source  

Bmf Lysate

Western Blot: Bmf Lysate [NBL1-07996] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for BMF Protein
  Novus Biologicals Inc.

BMF overexpression lysate

BMF overexpression lysate
0.1 mg / €295.00
  OriGene Technologies, Inc.
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