AM26349PU-N BPDE-DNA antibody

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0.1 mg / €480.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

Quick Overview

Mouse anti BPDE-DNA 5D11


Product Description for BPDE-DNA

Mouse anti BPDE-DNA 5D11.
Presentation: Purified
Product is tested for Paraffin Sections, Immunoprecipitation, Immunocytochemistry/Immunofluorescence, Enzyme Immunoassay.

Properties for BPDE-DNA

Product Category Primary Antibodies
Quantity 0.1 mg
Presentation Purified
Applications E, ICC/IF, IP, P
Clonality Monoclonal
Clone 5D11
Host Mouse
Isotype IgG2a
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

BPDE-I-DNA complexed with methylated BSA
Isotype control AM03096PU-N
Application Flow cytometry (8): Washed sperm was fixed in 2% paraformaldehyde and permeabilized with 0.2% triton x-100/0.1% sodium citrate. Samples were treated with protK and RNase. To denature DNA samples were incubated with 4n HCl. After blocking with 5% normal serum samples were incubated with mAb.
Immunoassays (4,7): Plates were coated with 50 ng/well BPDE-DNA in 50mM Tris-buffer pH7.5 o/n at 4°C. Plates were blocked 1% FCS. DNA samples, 4µg, were mixed with 5D11 and added to the well. Detection with GtαMs-IgG-AP for 90’at 37°C.
Immunoflourescence (8).
Immunoprecipitation (10).
Immunohistochemistry on paraffin sections (2,3,5,6,9): 5 µm sections were RNase and prot-K  treated. DNA was denatured with 4N HCl and neutralized with 50mM Tris base. Section was blocked with 1.5% normal horse serum: The typical starting working dilution is 1:10.

A number of chemicals, including polycyclic aromatic hydrocarbons (PAHs), have been shown to bind to DNA. This DNA damage can occur both early and late in the malignant process, thereby acting as an initiator and assisting in the progression of tumors. PAHs are released into the environment following incomplete combustion of organic materials. The most common sources of PAHs are from smoking and from consuming broiled or grilled foods. Human exposure to PAHs comes from various occupational, environmental, dietary and medicinal sources. Benzo[a]pyrene is a representative PAH. Antibodies to benzo[a]pyrenediol-epoxide modified DNA (BPDE-DNA) can be used to identify polycyclic aromatic hydrocarbon (PAH)-DNA adducts. Exposure to this group of compounds is believed to be carcinogenic.

Concentration 0.1 mg/ml
General Readings
  1. Santella, R et al; Monoclonal antibodies to DNA modified by benzo[a]pyrene diol epoxide. Carcinogenesis 1984, 5: 373.
  2. Zhang YJ, Weksler BB, Wang L, Schwartz J, Santella RM. Immunohistochemical detection of polycyclic aromatic hydrocarbon-DNA damage in human blood vessels of smokers and non-smokers. Atherosclerosis. 1998 Oct;140(2):325-31. PubMed PMID: 9862275.
  3. Rybicki BA, Rundle A, Savera AT, Sankey SS, Tang D. Polycyclic aromatic hydrocarbon-DNA adducts in prostate cancer. Cancer Res. 2004 Dec 15;64(24):8854-9. PubMed PMID: 15604244.
  4. Maisonnette C, Simon P, Hennion MC, Pichon V. Selective immunoclean-up followed by liquid chromatography for the monitoring of a biomarker of exposure to polycyclic aromatic hydrocarbons in urine at the ng l-1 level. J Chromatogr A. 2006 Jul 7;1120(1-2):185-93. Epub 2006 Jan 18. PubMed PMID: 16414055.
  5. Jurisicova A, Taniuchi A, Li H, Shang Y, Antenos M, Detmar J, et al. Maternal exposure to polycyclic aromatic hydrocarbons diminishes murine ovarian reserve via induction of Harakiri. J Clin Invest. 2007 Dec;117(12):3971-8. PubMed PMID: 18037991. (Free PMC Article available, 4 images available)
  6. Shinmura K, Iwaizumi M, Igarashi H, Nagura K, Yamada H, Suzuki M, et al. Induction of centrosome amplification and chromosome instability in p53-deficient lung cancer cells exposed to benzo[a]pyrene diol epoxide (B[a]PDE). J Pathol. 2008 Nov;216(3):365-74. doi: 10.1002/path.2422. PubMed PMID: 18788085.
  7. Chen, K et al; Modulation of benzo[a]pyrene-induced DNA adduct, cyclin D1 and PCNA in oral tissue by 1,4-phenylenebis(methylene)selenocyanate. BBRC 2009, 383:151.
  8. Ji G, Gu A, Zhou Y, Shi X, Xia Y, Long Y, et al. Interactions between exposure to environmental polycyclic aromatic hydrocarbons and DNA repair gene polymorphisms on bulky DNA adducts in human sperm. PLoS One. 2010 Oct 5;5(10). pii: e13145. doi: 10.1371/journal.pone.0013145. PubMed PMID: 20957144. (Free PMC Article available, 8 images available)
  9. Abedi-Ardekani B, Kamangar F, Hewitt SM, Hainaut P, Sotoudeh M, Abnet CC, et al. Polycyclic aromatic hydrocarbon exposure in oesophageal tissue and risk of oesophageal squamous cell carcinoma in north-eastern Iran. Gut. 2010 Sep;59(9):1178-83. doi: 10.1136/gut.2010.210609. Epub 2010 Jun 28. PubMed PMID: 20584779. (Free PMC Article available, 2 images available)
  10. Ye F, Xu XC. Benzo[a]pyrene diol epoxide suppresses retinoic acid receptor-beta2 expression by recruiting DNA (cytosine-5-)-methyltransferase 3A. Mol Cancer. 2010 Apr 28;9:93. doi: 10.1186/1476-4598-9-93. PubMed PMID: 20426865. (Free PMC Article available, 6 images available)

Store at 2 - 8 °C.
Shelf life: one year from despatch.

Protein G
Buffer System:
0.02% sodium azide
0.1% bovine serum albumin
Liquid 0.2 µm filtered Ig fraction
The monoclonal antibody 5D11 recognizes BPDE-I-DNA (PAH-DNA).

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