discontinued

AM39123PU-N BRCA1 / RNF53 antibody

See related secondary antibodies

Search for all "BRCA1 / RNF53"

Quick Overview

Mouse anti Human BRCA1 / RNF53 MS13

AM39123PU-N

More Views

  • AM39123PU-N
  • AM39123PU-N

Product Description for BRCA1 / RNF53

Mouse anti Human BRCA1 / RNF53 MS13.
Presentation: Purified
Product is tested for Western blot / Immunoblot, Paraffin Sections, Immunocytochemistry/Immunofluorescence.

Properties for BRCA1 / RNF53

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms Breast cancer type 1 susceptibility protein, RING finger protein 53
Presentation Purified
Reactivity Hu
Applications ICC/IF, P, WB
Clonality Monoclonal
Clone MS13
Host Mouse
Isotype IgG1
Molecular weight ~220 kDa observed. The calculated molecular weight of this protein is 208 kDa, but can be observed at ~220 kDa due to high glycosylation.
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P38398
Immunogen:
Recombinant protein corresponding to human BRCA1
GeneID:
672
Isotype control AM03095PU-N, SM10P (for use in human samples)
Application Western Blot: 0.5 µg/mL of this antibody detected BRCA1 on 10 µg of HeLa nuclear extract.
Immunohistochemistry: A 1:50 dilution from a representative lot detected BRCA1 in human breast cancer and human ovarian cancer tissues.
Immunocytochemistry: A 1:500 dilution from a representative lot detected BRCA1 in HeLa and A431 cells.
Background Breast cancer type 1 susceptibility protein (BCRA1; RING finger protein 53) functions as a tumor suppressor. BRCA1 is an important member of the DNA repair pathway. After DNA damage, BRCA1 is phosphorylated on multiple serine residues by the ATR kinase or Chk2, and is localized to lesion sites with PCNA. BRCA1 interacts with a wide range of proteins including Rad50, NBS1, and Mre11, c-Abl tyrosine kinase, and γH2A.X, involved in the detection of damaged DNA and activation of appropriate repair pathways. BRCA1 may also respond to DNA damage by promoting gene expression through association with transcriptional proteins and RNA polymerase II, and may regulate the p53 pathway by this mechanism. Previous studies have indicated that mutations in the BRCA1 gene may contribute to the development of breast and ovarian cancer.
Concentration 1.0 mg/ml (lot-specific)
Protocols Western Blotting
1. Perform SDS-polyacrylamide gel electrophoresis (SDS-PAGE) on cell lysate and transfer the proteins to a PVDF membrane. Wash the PVDF membrane twice with water.
2. Block the blotted PVDF membrane in freshly prepared 5% BSA or milk with 0.05% Tween®-20 surfactant for 1 hour at room temperature with constant agitation.
3. Incubate the PVDF with the recommended dilution of the primary antibody, diluted in freshly prepared 5% BSA or milk for 1 hour at room temperature or overnight with agitation at 2-8°C.
4. Wash the PVDF 3 times with TBST.
5. Incubate the PVDF in the secondary reagent of choice in 5% milk for 1 hour with agitation at room temperature.
6. Wash the PVDF 3-5 times with TBST.
7. Visualize with enhanced chemiluminescence (ECL) method of choice.
General Readings
  1. Cortez D, Wang Y, Qin J, Elledge SJ. Requirement of ATM-dependent phosphorylation of brca1 in the DNA damage response to double-strand breaks. Science. 1999 Nov 5;286(5442):1162-6. PubMed PMID: 10550055.
  2. Roy R, Chun J, Powell SN. BRCA1 and BRCA2: different roles in a common pathway of genome protection. Nat Rev Cancer. 2011 Dec 23;12(1):68-78. doi: 10.1038/nrc3181. PubMed PMID: 22193408.
Storage Store undiluted at 2-8°C.
Shelf life: One year from despatch.
Format
Purification:
Protein G chromatography
Buffer System:
0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide
State:
Liquid purified IgG1 fraction
Purified
Specificity
Specificity:
The antibody reacts with Human BRCA1.
Other species not tested.

Accessory Products

Proteins and/or Positive Controls

Proteins for BRCA1 / RNF53 (5 products)

Catalog No. Species Pres. Purity   Source  

BRCA1 / RNF53

BRCA1 / RNF53 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

BRCA1 / RNF53

BRCA1 / RNF53 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

BRCA1 / RNF53

BRCA1 / RNF53 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

BRCA1 / RNF53

BRCA1 / RNF53 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

BRCA1 / RNF53

BRCA1 / RNF53 Human Purified > 95 % Insect cells
2 µg / €620.00
  Neuromics Antibodies
  • LinkedIn