AP33262PU-N BRCA1 / RNF53 (N-term) antibody

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0.4 ml / €370.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

Quick Overview

Rabbit anti Human BRCA1 / RNF53

Product Description for BRCA1 / RNF53

Rabbit anti Human BRCA1 / RNF53.
Properties: (N-term)
Presentation: Aff - Purified
Product is tested for Western blot / Immunoblot.

Properties for BRCA1 / RNF53

Product Category Primary Antibodies
Target Category
Quantity 0.4 ml
Synonyms Breast cancer type 1 susceptibility protein, RING finger protein 53
Presentation Aff - Purified
Reactivity Hu
Applications WB
Clonality Polyclonal
Host Rabbit
Isotype Ig
Molecular weight 207721 Da
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P38398
Immunogen:
KLH conjugated synthetic peptide between 443-472 amino acids from the N-terminal region of Human BRCA1.
Property N-term
Application Western blot: 1000.
Background This gene encodes a nuclear phosphoprotein that plays a role in maintaining genomic stability, and it also acts as a tumor suppressor. The encoded protein combines with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as the BRCA1-associated genome surveillance complex (BASC). This gene product associates with RNA polymerase II, and through the C-terminal domain, also interacts with histone deacetylase complexes. This protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. Mutations in this gene are responsible for approximately 40% of inherited breast cancers and more than 80% of inherited breast and ovarian cancers. Alternative splicing plays a role in modulating the subcellular localization and physiological function of this gene. Many alternatively spliced transcript variants, some of which are disease-associated mutations, have been described for this gene, but the full-length natures of only some of these variants has been described. A related pseudogene, which is also located on chromosome 17, has been identified. [provided by RefSeq]. 
Cellular Location: Nucleus. Chromosome (By similarity). Note: Localizes at sites of DNA damage at double-strand breaks (DSBs); recruitment to DNA damage sites is mediated by the BRCA1-A complex Isoform 5: Cytoplasm. 
Tissue Location: Isoform 1 and isoform 3 are widely expressed. Isoform 3 is reduced or absent in several breast and ovarian cancer cell lines.
Concentration lot specific
General Readings
  1. Matsuoka S, Ballif BA, Smogorzewska A, McDonald ER, Hurov KE, Luo J, et al. ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage. Science. 2007 May 25;316(5828):1160-6. PubMed PMID: 17525332.
  2. Olsen JV, Blagoev B, Gnad F, Macek B, Kumar C, Mortensen P, et al. Global, in vivo, and site-specific phosphorylation dynamics in signaling networks. Cell. 2006 Nov 3;127(3):635-48. PubMed PMID: 17081983.
  3. Fabbro M, Savage K, Hobson K, Deans AJ, Powell SN, McArthur GA, et al. BRCA1-BARD1 complexes are required for p53Ser-15 phosphorylation and a G1/S arrest following ionizing radiation-induced DNA damage. J Biol Chem. 2004 Jul 23;279(30):31251-8. Epub 2004 May 24. PubMed PMID: 15159397.
  4. Ouchi M, Fujiuchi N, Sasai K, Katayama H, Minamishima YA, Ongusaha PP, et al. BRCA1 phosphorylation by Aurora-A in the regulation of G2 to M transition. J Biol Chem. 2004 May 7;279(19):19643-8. Epub 2004 Feb 27. PubMed PMID: 14990569.
  5. Orban TI, Olah E. Emerging roles of BRCA1 alternative splicing. Mol Pathol. 2003 Aug;56(4):191-7. PubMed PMID: 12890739. (Free PMC Article available, 1 images available)
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Protein A Chromatography, followed by peptide affinity purification
Buffer System:
PBS
Preservatives:
0.09% (W/V) Sodium Azide
State:
Liquid purified Ig fraction
Aff - Purified
Specificity
Specificity:
This antibody recognizes Human BRCA1/RNF53 (N-term). Other species not tested.

Accessory Products

Proteins and/or Positive Controls

Proteins for BRCA1 / RNF53 (5 products)

Catalog No. Species Pres. Purity   Source  

BRCA1 / RNF53

BRCA1 / RNF53 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

BRCA1 / RNF53

BRCA1 / RNF53 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

BRCA1 / RNF53

BRCA1 / RNF53 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

BRCA1 / RNF53

BRCA1 / RNF53 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

BRCA1 / RNF53

BRCA1 / RNF53 Human Purified > 95 % Insect cells
2 µg / €620.00
  Neuromics Antibodies
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