discontinued

NB100-599 BRCA1 / RNF53 antibody

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Mouse anti Human BRCA1 / RNF53 KEN

NB100-599

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  • NB100-599
  • NB100-599

Product Description for BRCA1 / RNF53

Mouse anti Human BRCA1 / RNF53 KEN.
Presentation: Purified
Product is tested for Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Western blot / Immunoblot.

Properties for BRCA1 / RNF53

Product Category Primary Antibodies
Target Category
Quantity 0.1 ml
Synonyms Breast cancer type 1 susceptibility protein, RING finger protein 53
Presentation Purified
Reactivity Hu
Applications ICC/IF, IP, WB
Clonality Monoclonal
Clone KEN
Host Mouse
Isotype IgG
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

Immunogen
Swiss Prot Num:
P38398
Immunogen:
Human BRCA1 (residues 1314-1864).
GeneID:
672
Add. information Research Areas: Cancer, DNA Repair
Application immunofluorescence, immunoprecipitation, Western Blot, A band can be seen ~220-240 kDa in Western blot. The investigator should determine the optimal working dilution for a specific application. , immunofluorescence , immunoprecipitation , Western Blot 2-4 ug/ml (ECL)
Background BRCA1 (breast and ovarian cancer susceptibility protein 1) is a RING finger protein containing a BRCT domain. BRCA1 exists as a heterodimer with 22 possible isoforms. The full length protein has a reported molecular weight of 208 kD. BRCA1 localizes to the mitotic spindle microtubules, centriole walls, pericentriolar fibers at centrosomes. Unphosphorylated BRCA1 localizes on chromosomes from metaphase through telophase; phosphorylated BRCA1 resides in inner chromosomal structure, centrosome, cleavage furrow during prophase through telophase, and relocalizes to the perinuclear region when cells are subjected to IR or UV radiation in S phase. BRCA1 acts as a tumor suppressor and can function as a secreted growth inhibitory protein, participate in transcription coupled repair of oxidative DNA damage, X-chromosome inactivation, and can function as a E3 ubiquitin ligase. BRCA1 can be transcriptionally downregulated by Ets-2, Brg-1, and Hmga-1. BRCA1 can be modified by glycosylation, ubiquitination and phosphorylation by CDK4, ATM/ATR, cdk2, and hChk2. The BRCA1 protein has been reported to interact with RNA polymerase II holoenzyme and BARD1. BRCA1 contains at least two nuclear localization signals and is proposed to be a tumor suppressor protein. It is a serine phosphoprotein that undergoes hyperphosphorylation during late G1 and S phases of the cell cycle and is transiently dephosphorylated early after M phase. BRCA1 protein alters in a qualitative and quantitative manner during cell cycle progression. The amount of BRCA1 protein is highest during S phase and remains elevated toward G2 / M, before it declines in early G1 phase. Inherited loss of BRCA1 function confers an increased susceptibility for both breast and ovarian cancer.
Concentration 1.67 mg/ml
General Readings
  1. Aglipay, et al. Oncogene. 22: 8931-8938 (2003)
  2. Okada, et al. JBC. 278: 2015-2020 (2003)
Storage Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Format
Purification:
protein G purified
Buffer System:
0.05% Sodium Azide
Purity:
protein G purified
State:
Purified
Specificity
Specificity:
This recognizes the epitope aa1314-1600.
Species:
Human
Gene ID 672

Accessory Products

Proteins and/or Positive Controls

Proteins for BRCA1 / RNF53 (5 products)

Catalog No. Species Pres. Purity   Source  

BRCA1 / RNF53

BRCA1 / RNF53 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

BRCA1 / RNF53

BRCA1 / RNF53 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

BRCA1 / RNF53

BRCA1 / RNF53 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

BRCA1 / RNF53

BRCA1 / RNF53 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

BRCA1 / RNF53

BRCA1 / RNF53 Human Purified > 95 % Insect cells
2 µg / €620.00
  Neuromics Antibodies
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