AP20475PU-N BUB3 antibody

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0.1 mg / €350.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

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Rabbit anti Human, Mouse, Rat BUB3

AP20475PU-N

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  • AP20475PU-N
  • AP20475PU-N

Product Description for BUB3

Rabbit anti Human, Mouse, Rat BUB3.
Presentation: Aff - Purified
Product is tested for Immunocytochemistry/Immunofluorescence, Paraffin Sections, Western blot / Immunoblot.

Properties for BUB3

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms Mitotic checkpoint protein BUB3
Presentation Aff - Purified
Reactivity Hu, Ms, Rt
Applications ICC/IF, P, WB
Clonality Polyclonal
Host Rabbit
Molecular weight ~ 40 kDa
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
O43684
Immunogen:
Synthetic peptide, corresponding to amino acids 251-300 of Human BUB3.
GeneID:
9184
Application Western blot: 1/500 - 1/1000.
Immunohistochemistry on paraffin sections: 1/50 - 1/200.
Immunofluorescence: 1/50 - 1/200.
Background The mitotic checkpoint protein Bub3 appears to be essential during normal mitotic progression, preventing premature sister chromatid separation, missegreation and aneuploidy. Bub3 is also important during G2 and early mitosis stages, promoting normal mitotic entry. Loss of Bub3 function causes slower progression through prophase, apparently due to mitotic cyclins A and B not being accumulated.
Concentration 1.0 mg/ml
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Affinity Chromatography using epitope-specific immunogen
Buffer System:
Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.
State:
Liquid purified Ig fraction ( > 95% pure by SDS-PAGE)
Aff - Purified
Species Reactivity
Species reactivity (expected):
Mouse and Rat.
Species reactivity (tested):
Human.
Specificity
Specificity:
This antibody detects endogenous levels of Bub3 protein.
(region surrounding His274)
Gene ID 9184

Accessory Products

Proteins and/or Positive Controls

Proteins for BUB3 (5 products)

Catalog No. Species Pres. Purity   Source  

BUB3 (transcript variant 2)

BUB3 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

BUB3 (1-328, His-tag)

BUB3 Human Purified > 85 % by SDS - PAGE E. coli
0.5 mg / €730.00
  Acris Antibodies GmbH

BUB3 (1-328, His-tag)

BUB3 Human Purified > 85 % by SDS - PAGE E. coli
0.1 mg / €300.00
  Acris Antibodies GmbH

BUB3

BUB3 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

BUB3

BUB3 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

Positive controls for BUB3 (2 products)

Catalog No. Species Pres. Purity   Source  

Bub3 Lysate

Western Blot: Bub3 Lysate [NBL1-08058] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for BUB3 Protein
  Novus Biologicals Inc.

BUB3 overexpression lysate

BUB3 overexpression lysate
0.1 mg / €295.00
  OriGene Technologies, Inc.
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