TA306431 BUB3 (N-term) antibody

Rabbit Polyclonal Bub3 Antibody

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0.1 mg / €415.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

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Rabbit anti Human, Mouse BUB3


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  • TA306431
  • TA306431

Product Description for BUB3

Rabbit anti Human, Mouse BUB3.
Properties: (N-term)
Presentation: Aff - Purified
Product is tested for Enzyme Immunoassay, Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot.

Properties for BUB3

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms Mitotic checkpoint protein BUB3
Presentation Aff - Purified
Reactivity Hu, Ms
Applications E, ICC/IF, WB
Clonality Polyclonal
Host Rabbit
Isotype IgG
Shipping to Europe, USA/Canada
PDF datasheet View Datasheet
Manufacturer OriGene Technologies, Inc.

Datasheet Extract

Swiss Prot Num:
Bub3 antibody was raised against a 16 amino acid peptide from near the carboxy terminus of human bub3. (AP30173CP-N)
Property N-term
Add. information Blocking peptide available: blocking peptide (AP30173CP-N)
Application ELISA.
Western Blot: Bub3 antibody can be used for detection of bub3 at 0.5 μg/ml.
Background The mitotic checkpoint protein Bub3 is involved with the essential spindle checkpoint pathway which operates during early embryogenesis. Bub3 is important during G2 and early mitosis stages, permitting entry into mitosis depending upon the assembly state of microtubules, thus preventing premature sister chromatid separation, mis-segregation and aneuploidy. Bub3 contains four WD repeat domains and is required for the kinetochore localization of Bub1, a related kinase that is necessary for spindle assembly checkpoint function. Bub1 is able to autophosphorylate and can catalyze the phosphorylation of Bub3. Both Bub1 and Bub3 are mutually dependent for function. Altered Bub expression levels may significantly impair mitotic checkpoint function and is associated with tumor cell proliferation.
General Readings
  1. Kalitsis P, Earle E, Fowler KJ, Choo KH. Bub3 gene disruption in mice reveals essential mitotic spindle checkpoint function during early embryogenesis. Genes Dev. 2000 Sep 15;14(18):2277-82. PubMed PMID: 10995385. (Free PMC Article available, 4 images available)
  2. Taylor SS, Ha E, McKeon F. The human homologue of Bub3 is required for kinetochore localization of Bub1 and a Mad3/Bub1-related protein kinase. J Cell Biol. 1998 Jul 13;142(1):1-11. PubMed PMID: 9660858. (Free PMC Article available, 9 images available)
  3. Warren CD, Brady DM, Johnston RC, Hanna JS, Hardwick KG, Spencer FA. Distinct chromosome segregation roles for spindle checkpoint proteins. Mol Biol Cell. 2002 Sep;13(9):3029-41. PubMed PMID: 12221113. (Free PMC Article available, 7 images available)
  4. Roberts BT, Farr KA, Hoyt MA. The Saccharomyces cerevisiae checkpoint gene BUB1 encodes a novel protein kinase. Mol Cell Biol. 1994 Dec;14(12):8282-91. PubMed PMID: 7969164. (Free PMC Article available)
Storage Store the antibody undiluted at 2-8°C.
Shelf life: one year from despatch.
Affinity chromatography purified via peptide column
Buffer System:
PBS containing 0.02% sodium azide.
Liquid purified Ig fraction
Aff - Purified
This antibody reacts to Bub3.
Human, Mouse.

Accessory Products

Proteins and/or Positive Controls

Proteins for BUB3 (5 products)

Catalog No. Species Pres. Purity   Source  

BUB3 (transcript variant 2)

BUB3 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €748.00
  OriGene Technologies, Inc.

BUB3 (1-328, His-tag)

BUB3 Human Purified > 85 % by SDS - PAGE E. coli
0.5 mg / €820.00
  OriGene Technologies GmbH

BUB3 (1-328, His-tag)

BUB3 Human Purified > 85 % by SDS - PAGE E. coli
0.1 mg / €320.00
  OriGene Technologies GmbH


BUB3 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.


BUB3 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

Positive controls for BUB3 (2 products)

Catalog No. Species Pres. Purity   Source  

Bub3 Lysate

Western Blot: Bub3 Lysate [NBL1-08058] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for BUB3 Protein
  Novus Biologicals Inc.

BUB3 overexpression lysate

BUB3 overexpression lysate
0.1 mg / €295.00
  OriGene Technologies, Inc.
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