AM00062PU-N c-fos (N-term) (incl. pos. control) antibody

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0.1 mg / €430.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

Quick Overview

Mouse anti Canine, Human, Mouse, Rat c-fos 8B5

Product Description for c-fos

Mouse anti Canine, Human, Mouse, Rat c-fos 8B5.
Properties: (N-term) (incl. pos. control)
Presentation: Purified
Product is tested for Frozen Sections, Enzyme Immunoassay, Western blot / Immunoblot.

Properties for c-fos

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms Cellular oncogene fos, FOS, G0/G1 switch regulatory protein 7, G0S7, Proto-oncogene protein c-fos
Presentation Purified
Reactivity Can, Hu, Ms, Rt
Applications C, E, WB
Clonality Monoclonal
Clone 8B5
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P01100
Immunogen:
Synthetic peptide conjugated to KLH.
GeneID:
2353
Property N-term
Isotype control AM03095PU-N, SM10P (for use in human samples), SM20P (for use in rat samples)
Application ELISA: 0.1 µg/ml (protein ELISA).
Immunohistochemistry on Frozen Sections
.
Western Blot
: 0.5 µg/ml for HRPO/ECL detection.
Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer.
Included Positive Control: Cell lysate from untreated HepG2 cells (See 'Protocols').
Background The immediated early gene product c-Fos is expressed following mitogenic stimulation. c-Fos functions as a sensor for MAPK signal duration. When MAPK activation is transient, MAPK activity declines before accumulation of the c-Fos protein. When MAPK activation is sustained, c-Fos is phosphorylated by MAPK at serine 374. Phosphorylation stabilizes the Fos protein and primes c-Fos for additional phosphorylation at threonine 325.
Protocols Positive Control: Cell lysate from untreated HepG2 cells.

Format: Lyophilized cell lysate from serum starved HepG2 cells.

Reconstitution: Restore by addition of 200 µl H2O. After complete solubilization add 200 µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min.

Application: The positive control cell lysate is recommended for immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 20.000 cells.
Use 20 µl/lane (mini gel) for HRPO/ECL detection of the target proteins.
Please NOTE: The lyophilized cell lysates conatin SDS and are not recommended for applications with native proteins such as in immunoprecipitation.

Storage: Aliquote reconstituted product and store frozen. Avoid repeated fereezing and thawing.
Storage Store lyophilized (preferably in a desiccator) at -20°C and reconstituted (aliquote and freeze in liquid nitrogen) at -80°C.
Avoid repeated freezing and thawing.
Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months.
Shelf life: one year from despatch
Format
Purification:
Subsequent Thiophilic Adsorption and Size Exclusion Chromatography.
Buffer System:
2x PBS containing 0.09% Sodium Azide, PEG and Sucrose
State:
Lyophilized purified IgG fraction
Purified
Reconstitution:
Restore with 1 ml H2O (15 min, RT).
Specificity
Specificity:
This antibody specifically interacts with the N-terminus of c-Fos. It can be used for the detection of cellular Fos levels that might dramatically change during signal transduction. It is an important tool in combination with mab Fos-34E4 (phospho-Ser374) for studying fos expression and phosphorylation.
Species:
Human, Mouse, Rat, Dog.
Gene ID 2353

Accessory Products

Proteins and/or Positive Controls

Proteins for c-fos (6 products)

Catalog No. Species Pres. Purity   Source  

c-fos (N-term HIS tag)

c-fos Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
50 µg / €199.00
  OriGene Technologies, Inc.

c-fos

c-fos Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

c-fos

c-fos Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

c-fos

c-fos Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

c-fos

c-fos Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

c-fos

c-fos Human
  Abnova Taiwan Corp.

Positive controls for c-fos (1 products)

Catalog No. Species Pres. Purity   Source  

c-Fos Lysate

Western Blot: c-Fos Lysate [NBL1-10794] - Western Blot experiments.  Left-Control; Right -Over-expression Lysate for FOS.
  Novus Biologicals Inc.
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