NB120-4765 c-Raf antibody

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Rabbit anti Human c-Raf


Product Description for c-Raf

Rabbit anti Human c-Raf.
Presentation: Aff - Purified
Product is tested for Western blot / Immunoblot.

Properties for c-Raf

Product Category Primary Antibodies
Quantity 0.05 ml
Presentation Aff - Purified
Reactivity Hu
Applications WB
Clonality Polyclonal
Host Rabbit
Isotype IgG
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

Synthetic peptide (Human) derived from a region of human c-Raf that contains serine 43. The sequence is conserved in mouse and rat.
Background The Raf family of serine/threonine-specific kinases is comprised of three members (A-Raf, B-Raf, and c-Raf) that play a critical role in regulating cell growth and differentiation, and couple growth factor receptor stimulation to nuclear transcription factors via the Ras/mitogen-activated protein kinase (MAPK) pathway. c-Raf kinase (also known as Raf-1) is a key 74 kDa signal transducer of multiple extracellular stimuli that is regulated by several pathways, and that once activated phosphorylates MEK which in turn phosphorylates ERK. Serine 43 of c-Raf is phosphorylated by PKA, and is thought to negatively affect the ability of Ras to activate c-Raf.
General Readings General / background references:Dumaz N et al. Cyclic AMP blocks cell growth through Raf-1-dependent and Raf-1-independent mechanisms. Mol Cell Biol 22:3717-28 (2002). PubMed PMID: 11997508
Zhang BH & Guan KL Regulation of the Raf kinase by phosphorylation. Exp Lung Res 27:269-95 (2001). PubMed PMID: 11293329
Ramstad C et al. cAMP-dependent protein kinase (PKA) inhibits T cell activation by phosphorylating ser-43 of raf-1 in the MAPK/ERK pathway. Cell Signal 12:557-63 (2000). PubMed PMID: 11027949
Morrison DK et al. Identification of the major phosphorylation sites of the Raf-1 kinase. J Biol Chem 268:17309-16 (1993). PubMed PMID: 8349614
Immunogen affinity purified
Buffer System:
Preservative: 0.05% Sodium AzideConstituents: PBS, 1.0mg/ml BSA (IgG, protease free). pH 7.3
Immunogen affinity purified
Aff - Purified
Cross-reacts with Human.Expected to cross-react with Mouse (100% identity with immunogen), Rat (100% identity with immunogen) due to sequence homology.Not yet tested in other species.

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