NB120-4766 c-Raf antibody

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Rabbit anti Human c-Raf


Product Description for c-Raf

Rabbit anti Human c-Raf.
Presentation: Aff - Purified
Product is tested for Western blot / Immunoblot.

Properties for c-Raf

Product Category Primary Antibodies
Quantity 0.05 ml
Presentation Aff - Purified
Reactivity Hu
Applications WB
Clonality Polyclonal
Host Rabbit
Isotype IgG
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

Synthetic peptide (Human) derived from a region of human c-Raf that contains serine 259. The sequence is conserved in mouse and rat.
Background The Raf family of serine/threonine-specific kinases is comprised of three members (A-Raf, B-Raf, and c-Raf) that play a critical role in regulating cell growth and differentiation, and couple growth factor receptor stimulation to nuclear transcription factors via the Ras/mitogen-activated protein kinase (MAPK) pathway. c-Raf kinase (also known as Raf-1) is a key 74 kDa signal transducer of multiple extracellular stimuli that is regulated by several pathways, and that once activated, phosphorylates MEK which in turn phosphorylates ERK. Serine 259 is one of the three constitutive phosphorylation sites of c-Raf in resting cells together with serine 43 and serine 621. Serine 259 is phosphorylated by PKA and is the main mediator of PKA-induced inhibition of c-Raf kinase activity. Phosphorylation of serine 259 blocks the membrane localization of c-Raf.
General Readings General / background references:Dhillon AS et al. Cyclic AMP-dependent kinase regulates Raf-1 kinase mainly by phosphorylation of serine 259. Mol Cell Biol 22:3237-46 (2002). PubMed PMID: 11971957
Dumaz N et al. Cyclic AMP blocks cell growth through Raf-1-dependent and Raf-1-independent mechanisms. Mol Cell Biol 22:3717-28 (2002). PubMed PMID: 11997508
Zhang BH & Guan KL Regulation of the Raf kinase by phosphorylation. Exp Lung Res 27:269-95 (2001). PubMed PMID: 11293329
Morrison DK et al. Identification of the major phosphorylation sites of the Raf-1 kinase. J Biol Chem 268:17309-16 (1993). PubMed PMID: 8349614
Immunogen affinity purified
Buffer System:
Preservative: 0.05% Sodium AzideConstituents: PBS, 1.0mg/ml BSA (IgG, protease free). pH 7.3
Immunogen affinity purified
Aff - Purified
Cross-reacts with Human.Expected to cross-react with Mouse (100% identity with immunogen), Rat (100% identity with immunogen) due to sequence homology.Not yet tested in other species.

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