NB120-4769 c-Raf antibody

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Rabbit anti Human, Mouse, Rat c-Raf


Product Description for c-Raf

Rabbit anti Human, Mouse, Rat c-Raf.
Presentation: Aff - Purified
Product is tested for Western blot / Immunoblot.

Properties for c-Raf

Product Category Primary Antibodies
Quantity 0.05 ml
Presentation Aff - Purified
Reactivity Hu, Ms, Rt
Applications WB
Clonality Polyclonal
Host Rabbit
Isotype IgG
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

Synthetic peptide derived from a region of c-Raf that contains tyrosines 340 and 341.
Background c-Raf, also known as Raf-1, a member of the MEK kinase family, is a 74 kDaserine/threonine kinase which is activated in response to a variety of mitogens and cytokines. Activation of Raf kinase activity is induced by several events including phosphorylation at several sites and binding of GTP-bound Ras and/or 14-3-3 proteins. This, in turn, leads to the phosphorylation and activation of other members of the MEK/MAPK/ERK cascade. Phosphorylation of tyrosines at position 340 and 341 iscatalyzed by the Src tyrosine kinase and this event is involved in c-Raf activation.
General Readings This product has been used in:Yan F & Polk DB Kinase suppressor of ras is necessary for tumor necrosis factor alpha activation of extracellular signal-regulated kinase/mitogen-activated protein kinase in intestinal epithelial cells. Cancer Res 61:963-9 (2001). PubMed PMID: 11221891
General / background references:Xing HR & Kolesnick R Kinase suppressor of Ras signals through Thr269 of c-Raf-1. J Biol Chem 276:9733-41 (2001). PubMed PMID: 11134016
Yip-Schneider MT et al. Regulation of the Raf-1 kinase domain by phosphorylation and 14-3-3 association. Biochem J 351:151-9 (2000). PubMed PMID: 10998357
Marais R et al. Differential regulation of Raf-1, A-Raf, and B-Raf by oncogenic ras and tyrosine kinases. J Biol Chem 272:4378-83 (1997). PubMed PMID: 9020159
Immunogen affinity purified
Buffer System:
Preservative: 0.05% Sodium AzideConstituents: PBS, 1.0mg/ml BSA (IgG, protease free). pH 7.3
Immunogen affinity purified
Aff - Purified
Cross-reacts with Human, Mouse and Rat.Not yet tested in other species.

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