NB110-61011 c-Raf Phosphospecific antibody

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Rabbit anti Human, Mouse, Rat c-Raf Phosphospecific


Product Description for c-Raf Phosphospecific

Rabbit anti Human, Mouse, Rat c-Raf Phosphospecific.
Presentation: Aff - Purified
Product is tested for Western blot / Immunoblot.

Properties for c-Raf Phosphospecific

Product Category Primary Antibodies
Quantity 10 µg
Presentation Aff - Purified
Reactivity Hu, Ms, Rt
Applications WB
Clonality Polyclonal
Host Rabbit
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human c-Raf that contains serine 621.
Application Western Blot 1:1000,
Background The Raf family of serine/threonine-specific kinases is comprised of three members (A-Raf, B-Raf, and c-Raf) that play a critical role in regulating cell growth and differentiation, and couple growth factor receptor stimulation to nuclear transcription factors via the Ras/mitogen-activated protein kinase (MAPK) pathway. c-Raf kinase (also known as Raf-1) is a key 74 kDa signal transducer of multiple extracellular stimuli that is regulated by several pathways, and that once activated phosphorylates MEK which in turn phosphorylates ERK. Together with serine 43 and serine 259, serine 621 is one of the three constitutive phosphorylation sites of c-Raf in resting cells. Phosphorylation of serine 621 has been shown to positively or negatively regulate c-Raf kinase activity, depending on the system.
General Readings Schulze, A., et al. (2004) The transcriptional response to Raf activation is almost completely dependent on Mitogen-activated Protein Kinase Kinase activity and shows a major autocrine component. Mol. Biol. Cell. 15(7):3450-3463.
Dhillon, A.S., et al. (2002) Cyclic AMP-dependent protein kinase regulates Raf-1 kinase mainly by phosphorylation of serine 259. Mol. Cell. Biol. 22(10):3237-3246.
Morel, J.C., et al. (2002) Signal transduction pathways involved in rheumatoid arthritis synovial fibroblast interleukin-18-induced vascular cell adhesion molecule-1 expression. J. Biol. Chem. 277(38):34679-34691.
Yan, F. and D.B. Polk (2001) Kinase suppressor of ras is necessary for tumor necrosis factor alpha activation of extracellular signal-regulated kinase/mitogen-activated protein kinase in intestinal epithelial cells. Cancer Res. 61(3):963-969.
Gu, M., et al. (2000) Nitric oxide increases p21Waf1/Cip1 expression by a cGMP-dependent pathway that includes activation of extracellular signal-regulated kinase and p70S6k. J. Biol. Chem. 275:11389-11396.
Thorson, J.A., et al. (1998) 14-3-3 Proteins are required for maintenance of Raf-1 phosphorylation and kinase activity. Mol. Cell. Biol. 18:5229-5238.
Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Affinity purified
Buffer System:
PBS, pH 7.3, 50% glycerol with 1.0 mg/ml BSA (IgG, protease free).
Affinity purified
Aff - Purified
Human. Expected to react in rat and mouse based on 100% homology. Not yet tested in other species.

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