discontinued

AM32502SU-N Calmodulin Binding Protein Epitope Tag antibody

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0.1 ml / €400.00

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Rabbit anti Vertebrates Calmodulin Binding Protein Epitope Tag C16T

Product Description for Calmodulin Binding Protein Epitope Tag

Rabbit anti Vertebrates Calmodulin Binding Protein Epitope Tag C16T.
Presentation: Purified
Product is tested for Western blot / Immunoblot.

Properties for Calmodulin Binding Protein Epitope Tag

Product Category Primary Antibodies
Quantity 0.1 ml
Synonyms CBP
Presentation Purified
Reactivity Ve
Applications WB
Clonality Monoclonal
Clone C16T
Host Rabbit
Isotype IgG
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
KLH-conjugated, synthetic peptide corresponding to the Calmodulin Binding Protein (CBP) epitope tag found in several E. coli expression vectors.
Isotype control PP501P
Application Immunoblot Analysis: 1/5,000-1/15,000 dilution of this lot detected a recombinant protein containing the calmodulin binding protein epitope tag in lysates from transformed E. coli.
Control:
Samples containing the Calmodulin Binding Protein Epitope tag.
Quality Assurance: Routinely evaluated by immunoblot on E. coli transformed with a plasmid encoding a Calmodulin Binding Protein Epitope tagged protein.
Protocols Immunoblot Protocol
1. Perform SDS-polyacrylamide gel electrophoresis (SDS-PAGE) on a transfected cell lysate sample (cell lysis buffer: 50 mM Tris-HCl, pH 7.4; 1% NP-40; 0.25% sodium deoxycholate; 150 mM NaCl; 1 mM EDTA; 1 mM PMSF; 1 μg/mL each aprotinin, leupeptin, pepstatin; 1 mM Na3VO4, 1 mM NaF) and transfer the proteins to nitrocellulose. Wash the blotted nitrocellulose twice with water.
2. Block the blotted nitrocellulose in freshly prepared TBS containing 3% nonfat dry milk in TBS with 0.05% Tween®-20 (TBST-MLK) for 1 hour at room temperature with constant agitation.
3. Incubate the nitrocellulose with a 1:5,000-1:15,000 dilution of anti-Calmodulin Binding Protein Epitope Tag, diluted in freshly prepared TBST-MLK for 2 hours at room temperature with constant agitation.
4. Wash the nitrocellulose twice with water.
5. Incubate the nitrocellulose in the secondary reagent of choice (a goat anti-rabbit HRP-conjugated IgG, 1:5000 dilution was used) in TBST-MLK for 1 hour at room temperature with agitation.
6. Wash the nitrocellulose twice with water.
7. Wash the nitrocellulose in TBS-0.05% Tween®-20 for 3-5 minutes.
8. Rinse the nitrocellulose in 4-5 changes of water.
9. Use detection method of choice (enhanced chemiluminescence was used).
General Readings
  1. Zheng CF, Simcox T, Xu L, Vaillancourt P. A new expression vector for high level protein production, one step purification and direct isotopic labeling of calmodulin-binding peptide fusion proteins. Gene. 1997 Feb 20;186(1):55-60. PubMed PMID: 9047344.
Storage Store undiluted (in aliquots) at -20°C.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Protein A Chromatography
Buffer System:
0.07M Tris-glycine, pH 7.4, 0.105 M NaCl
Preservatives:
0.035% Sodium Azide
Stabilizers:
30% Glycerol
State:
Liquid purified IgG fraction
Purified
Species Reactivity
Species reactivity (tested):
Vertebrates.
Specificity
Specificity:
Recognizes Recombinant proteins containing the Calmodulin Binding Protein Epitope Tag.

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