AM05265PU-N Calpain (CAPN1+CAPN2) antibody

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0.1 mg / €360.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

Quick Overview

Mouse anti Bovine, Human Calpain 28F3

AM05265PU-N

Product Description for Calpain

Mouse anti Bovine, Human Calpain 28F3.
Properties: (CAPN1+CAPN2)
Presentation: Purified
Product is tested for Enzyme Immunoassay, Immunoprecipitation, Western blot / Immunoblot.

Properties for Calpain

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms CANP-2, CANP1, CANPL1, CANPL2, CAPN1, CAPN2
Presentation Purified
Reactivity Bov, Hu
Applications E, IP, WB
Clonality Monoclonal
Clone 28F3
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Calpain purified from human placenta.
Property CAPN1+CAPN2
Isotype control AM03095PU-N, SM10P (for use in human samples)
Application ELISA: 0.5-1 ug/ml.
Western blot: 0.5-1 ug/ml.
Immunoprecipitation of both the native and denatured protein: 1-2 ug/ml.
Background The calpains are calcium-dependent cysteine proteases that are widely expressed in mammalian systems. Both m-calpain (calpain II) and -calpain (calpain I) are composed of an 80 kD subunit and a 30 kD subunit. Whereas the 30 kDa subunit is shared by both enzymes, the larger catalytic subunits are different and exhibit the distinct Ca ++ requirements that are suggested by their names. Whereas m-calpain requires millimolar (mM) levels of calcium, u-calpain is active at micromolar (uM) concentrations of Ca++. In addition to the ubiquitously expressed m- and u-calpains, some tissue-specific calpains have been identified. The calpains appear to serve multiple physiological roles, and ideas concerning the functions of these enzymes are in a state of rapid flux.
Concentration Lot specific
Storage Store the antibody at -20°C.
Avoid repeated freezing and thawing.
Shelf life: One year from despatch.
Format
Purification:
Standard chromatographic techniques.
Buffer System:
0.02 M Sodium Phosphate, pH 7.5, 0.15 M Sodium Chloride, 50% Glycerol and 3 mM Sodium Azide as presevtative.
Purity:
Purified
State:
Liquid (sterile filtered) purified IgG fraction (> 95% pure).
Purified
Specificity
Specificity:
This antibody specifically recognizes the 28-30 kD subunit of m-calpain and u-calpain.
React with human and bovine calpains, but does not recognize calpains from rat or mouse.
Species:
Human and Bovine.

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