DM345P Calponin antibody

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Mouse anti Human, Rat Calponin CALP


Product Description for Calponin

Mouse anti Human, Rat Calponin CALP.
Presentation: Purified
Product is tested for Western blot / Immunoblot, Paraffin Sections.

Properties for Calponin

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms AB700
Presentation Purified
Reactivity Hu, Rt
Applications P, WB
Clonality Monoclonal
Clone CALP
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Biomeda

Datasheet Extract

Crude Human Uterus extract
Epitope not determined.
Molecular weight of antigen 34 kD
Isotype control AM03095PU-N, SM10P (for use in human samples), SM20P (for use in rat samples)
Application Immunohistology (Formalin/paraffin): use at 0.5-1.0 µg/ml for 30 min at RT. Staining of formalin-fixed tissues requires digestion of tissue sections with trypsin at 1 mg/ml Tris-HCl, pH 7.4, for 10-15 min at RT, followed by boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. Recommended positive control: myoepithelial cells in breast ducts.
Cellular localization: cytoplasmic
Background Calponin, a calmodulin, binds tropomyosin and F-actin and is thought to be involved in the regulation of smooth muscle contraction. The expression of calponin is restricted to smooth muscle cells and is a marker of the differentiated contractile phenotype of developing smooth muscle. Two isoforms of calponin exist whose molecular weights are 34 kD and 29 kD. Expression of the 29 kD form is primarily restricted to muscle of the urogenital tract. The expression of calponin has also been demonstrated in myoepithelialcells from benign and malignant breast lesions.
Concentration 0.2 mg/ml
Storage Store the antibody at 4°C. Do not freeze!
Protein G affinity chromatography
Buffer System:
10 mM PBS, pH 7.4, containing 0.09% sodium azide as preservative and 0.2% BSA as stabilizer
Liquid Ig fraction
Human, rat

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