BM6044P Cardiotin antibody

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0.1 mg / €320.00
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Quick Overview

Mouse anti Human, Porcine Cardiotin SR-2

BM6044P

Product Description for Cardiotin

Mouse anti Human, Porcine Cardiotin SR-2.
Presentation: Purified
Product is tested for Frozen Sections, Western blot / Immunoblot, Paraffin Sections.

Properties for Cardiotin

Product Category Primary Antibodies
Quantity 0.1 mg
Presentation Purified
Reactivity Hu, Por
Applications C, P, WB
Clonality Monoclonal
Clone SR-2
Host Mouse
Isotype IgM
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
100 kDa Cardiotin subunit
Isotype control SM13P
Application Immunoblotting. 
Immunohistochemistry on Frozen Sections.
Immunohistochemistry on Paraffin-Embedded Tissue.
Recommended Dilutions: 1/25–1/100 for immunohistochemistry with ABC as detection reagent, and 1/50–1/500 for immunoblotting.
Background Cardiotin is a high molecular weight protein complex (300 kDa) located in the mitochondria of cardiomyocytes and skeletal muscle. The cardiotin structure exists of subunits of 60 kDa and 100 kDa, probably in a tetrameric configuration. Both subunits contain the same amino-terminal 14 amino-acid sequence, showing high homology to human skeletal muscle α-actinin.
During cardiac contractile dysfunction and myocard cell differentiation, the cardiotin distribution is affected. Compared to other structural proteins, cardiotin is one of the first to respond to insults (ischemia, fibrillation) that influence the functional status of cardiomyocytes.
Concentration 1 mg/ml
General Readings
  1. Schaart G, Moens L, Endert JM, Ramaekers FC. Biochemical characterization of cardiotin, a sarcoplasmic reticulum associated protein. FEBS Lett. 1997 Feb 17;403(2):168-72. PubMed PMID: 9042960.
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Buffer System:
PBS
Preservatives:
0.09% Sodium Azide
State:
Liquid purified Ig fraction
Purified
Species Reactivity
Species reactivity (tested):
Human, Swine.
Specificity
Specificity:
This antibody SR-2 reacts with Cardiotin, a mitochondrionassociated protein, which is present in cardiomyocytes and skeletal muscle. SR-2 reacts with cardiomyocytes, skeletal muscle, stromal and epithelial cells as well in vivo as in vitro. 
 In immunoblotting assays SR-2 reacts with the 300 kDa cardiotin protein complex and its 100 kDa and 60 kDa subunits.
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