BM6052P Cardiotin antibody

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0.1 mg / €320.00
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Quick Overview

Mouse anti Human, Porcine Cardiotin SR-4

Product Description for Cardiotin

Mouse anti Human, Porcine Cardiotin SR-4.
Presentation: Purified
Product is tested for Frozen Sections, Western blot / Immunoblot.

Properties for Cardiotin

Product Category Primary Antibodies
Quantity 0.1 mg
Presentation Purified
Reactivity Hu, Por
Applications C, WB
Clonality Monoclonal
Clone SR-4
Host Mouse
Isotype IgM
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Derived by fusion of SP2/0-Ag14 mouse myeloma cells with spleen cells from a BALB/c mouse immunized with cardiotin.
Application Immunoblotting: 1/25-1/250.
Flow Cytometry: 1/25-1/50. 
Immunohistochemistry on Frozen Sections: 1/25-1/50 with ABC as detection reagent.
Background Cardiotin is a high molecular weight protein complex (300 kDa) located in the mitochondria of cardiomyocytes and skeletal muscle. The cardiotin structure exists of subunits of 60 kDa and 100 kDa, probably in a tetrameric configuration. Both subunits contain the same amino-terminal 14 amino-acid sequence, showing high homology to human skeletal muscle α-actinin.
During cardiac contractile dysfunction and myocard cell differentiation, the cardiotin distribution is affected. Compared to other structural proteins, cardiotin is one of the first to respond to insults (ischemia, fibrillation) that influence the functional status of cardiomyocytes.
Concentration 1.0 mg/ml
General Readings
  1. Schaart G, van der Ven PF, Ramaekers FC. Characterization of cardiotin, a structural component in the myocard. Eur J Cell Biol. 1993 Oct;62(1):34-48. PubMed PMID: 8269977.
  2. Schaart G, Moens L, Endert JM, Ramaekers FC. Biochemical characterization of cardiotin, a sarcoplasmic reticulum associated protein. FEBS Lett. 1997 Feb 17;403(2):168-72. PubMed PMID: 9042960.
  3. Ausma J, Wijffels M, van Eys G, Koide M, Ramaekers F, Allessie M, et al. Dedifferentiation of atrial cardiomyocytes as a result of chronic atrial fibrillation. Am J Pathol. 1997 Oct;151(4):985-97. PubMed PMID: 9327732. (Free PMC Article available)
  4. Dispersyn GD, Geuens E, Ver Donck L, Ramaekers FC, Borgers M. Adult rabbit cardiomyocytes undergo hibernation-like dedifferentiation when co-cultured with cardiac fibroblasts. Cardiovasc Res. 2001 Aug 1;51(2):230-40. PubMed PMID: 11470462.
  5. Ausma J, Litjens N, Lenders MH, Duimel H, Mast F, Wouters L, et al. Time course of atrial fibrillation-induced cellular structural remodeling in atria of the goat. J Mol Cell Cardiol. 2001 Dec;33(12):2083-94. PubMed PMID: 11735256.
  6. Dispersyn GD, Mesotten L, Meuris B, Maes A, Mortelmans L, Flameng W, et al. Dissociation of cardiomyocyte apoptosis and dedifferentiation in infarct border zones. Eur Heart J. 2002 Jun;23(11):849-57. PubMed PMID: 12042006.
  7. Ausma, J., van der Velden, H. M., Lenders, M. H., van Ankeren, E. P., Jongsma, H. J., Ramaekers, F. C., Borgers, M., and Allessie, M. A. (2003). Reverse structural and gap-junctional remodeling after prolonged atrial fibrillation in the goat. Circulation
Storage Store undiluted at 2-8°C for one month or (in small aliquots) at -20°C for longer.
Avoid repeated freeze-thaw cycles.
Shelf life: one year from despatch.
Buffer System:
0.09% Sodium Azide
Liquid purified Ig fraction
Species Reactivity
Species reactivity (tested):
Human and Swine.
This antibody SR-4 reacts with Cardiotin, a mitochondrion-associated protein, which is present in cardiomyocytes and skeletal muscle, but also in epithelial cells and tissues.
SR-4 recognizes exclusively the 300 kDa Cardiotin protein complex by Immunoblotting.
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