AP05927PU-N Caspase-5 antibody

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0.1 mg / €410.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

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Rabbit anti Human Caspase-5

AP05927PU-N

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  • AP05927PU-N

Product Description for Caspase-5

Rabbit anti Human Caspase-5.
Presentation: Purified
Product is tested for Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot.

Properties for Caspase-5

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms CASP-5, CASP5, ICE(rel)-III, ICH-3 protease, ICH3, TY protease
Presentation Purified
Reactivity Hu
Applications ICC/IF, WB
Clonality Polyclonal
Host Rabbit
Isotype IgG
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P51878
Immunogen:
A 14 amino acid peptide from the centre of human Caspase-5.
GeneID:
838
Application Western blot: 1.0 µg/ml - 2.0 µg/ml; detects a band of approximately 48 kDa in Ramos cell lysates. (Predicted precursor MWT 47.8kDa).
Immuncytochemistry.
Background Caspase-5 has been shown to be expressed at higher levels in the colon, lung, spleen, small intestine and by peripheral blood lymphocytes.
Caspase-5 is a member of a growing number of inflammatory caspases belonging to the Caspase-1 (ICE-type) subfamily, which require proteolytic processing of their inactive precursor into smaller active subunits. Unlike Caspase-1, Caspase-5 is not directly responsible for the activation of IL-1Beta from the pro-IL-1Beta precursor, but the presence of Caspase-5 does appear to enhance the processing of IL-1Beta.
The in vivo expression of Caspase-5 is elevated by Lipopolysaccharide (LPS) and gene expression is also increased by Interferon gamma (IFNg). In vitro studies have shown cleavage of Caspase-5 by the serine protease Granzyme B, which plays a major role in Cytotoxic T lymphocyte (CTL)-induced apoptosis (1).
Caution (A full Health and Safety assessment is available upon request) This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Concentration 1.0 mg/ml
General Readings 1. Wang, S. et al. (1996) Identification and characterization of Ich-3, a member of the Interleukin-1beta converting enzyme (ICE)/Ced-3 family and an upstream regulator of ICE. J. Biol. Chem. 271: 20580-20587.
2. Lin, X.Y. et al. (2000) Expression analysis of the human Caspase-1 subfamily reveals specific regulation of the
CASP5 gene by Lipopolysaccharide and Interferon-gamma. J. Biol. Chem. 275: 39920-39926.
3. Munday, N.A. et al. (1995) Molecular cloning and pro-apoptotic activity of ICErelII and ICErelIII, members of the ICE/CED-3 family of cysteine proteases. J. Biol. Chem. 270: 15870-15876.
4. Cohen, G.M. (1997) Caspases: the executioners of apoptosis. Biochem. J. 326: 1-16.
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Affinity chromatography
Buffer System:
Phosphate buffered saline containing 0.02% Sodium Azide
Purity:
Antisera to human Caspase-5 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
State:
Liquid purified Ig
Purified
Specificity
Specificity:
This antibody recognises an epitope located within the internal region of human Caspase-5, a 47kDa cysteine protease, otherwise known as ICH-3.
Species:
Human.
Gene ID 838
FocusOn and Reviews
FocusOns:

Accessory Products

Proteins and/or Positive Controls

Proteins for Caspase-5 (4 products)

Catalog No. Species Pres. Purity   Source  

Caspase-5 (full length, N-term HIS tag, transcript variant a)

Caspase-5 Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
50 µg / €205.00
  OriGene Technologies, Inc.

Caspase-5

Caspase-5 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

Caspase-5

Caspase-5 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

Caspase-5

Caspase-5 Human
  Abnova Taiwan Corp.

Positive controls for Caspase-5 (3 products)

Catalog No. Species Pres. Purity   Source  

CASP5 overexpression lysate

CASP5 overexpression lysate
0.1 mg / €295.00
  OriGene Technologies, Inc.

CASP5 overexpression lysate

CASP5 overexpression lysate
0.1 mg / €315.00
  OriGene Technologies, Inc.

Caspase 5 Lysate

Western Blot: Caspase 5 Lysate [NBL1-08714] - Western Blot experiments.  Left-Control; Right -Over-expression Lysate for CASP5.
  Novus Biologicals Inc.
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