TA319431 CBX4 / PC2 antibody

Rabbit polyclonal anti-PC2 antibody

See related secondary antibodies

Search for all "CBX4 / PC2"

0.1 mg / €503.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

Quick Overview

Rabbit anti Human, Mouse CBX4 / PC2

TA319431

More Views

  • TA319431

Product Description for CBX4 / PC2

Rabbit anti Human, Mouse CBX4 / PC2.
Presentation: Aff - Purified
Product is tested for Enzyme Immunoassay, Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot.

Properties for CBX4 / PC2

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms Chromobox protein homolog 4, E3 SUMO-protein ligase CBX4, Polycomb 2 homolog
Presentation Aff - Purified
Reactivity Hu, Ms
Applications E, ICC/IF, WB
Clonality Polyclonal
Host Rabbit
Shipping to Europe, USA/Canada
PDF datasheet View Datasheet
Manufacturer OriGene Technologies, Inc.

Datasheet Extract

Immunogen
Swiss Prot Num:
O00257
Immunogen:
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to aa 95-107 of Human PC2 protein.
GeneID:
8535
Add. information Protein Sequence: Human PC2, 560 aa, predicted MW 61.4 kDa
1 melpavgehv favesiekkr irkgrveylv kwrgwspkyn twepeenild prlliafqnr
61 erqeqlmgyr krgpkpkplv vqvptfarrs nvltglqdss tdnrakldlg aqgkgqghqy
121 elnskkhhqy qphskeragk ppppgksgky yyqlnskkhh pyqpdpkmyd lqyqgghkea
181 psptcpdlga kshppdkwaq gagakgylga vkplagaaga pgkgsekgpp ngmmpapkea
241 vtgngiggkm kivknknkng rivivmskym engmqavkik sgevaegear spshkkraad
301 erhppadrtf kkaagaeekk veappkrree evsgvsdpqp qdagsrklsp tkeafgeqpl
361 qlttkpdlla wdparnthpp shhphphphh hhhhhhhhhh avglnlshvr krclsethge
421 repckkrlta rsistptclg gspaaerpad lppaaalpqp evilldsdld epidlrcvkt
481 rseageppss lqvkpetpas aavavaaaaa ptttaekppa eaqdepaesl sefkpffgni
541 iitdvtancl tvtfkeyvtv
Application This affinity purified antibody has been tested for use in immunofluorescence staining of cultured cells and western blotting using transfected cell lysates. The antibody is also functional by ELISA. Reactivity in other immunoassays is unknown. Dilutions for western blotting represent a starting point dilution and further optimization may be required. The antibody detects a band of approximately 82 kDa (predicted molecular weight: 61.4 kDa). In immunofluoresence, this antibody detects the expected discrete nuclear structure that is termed the PcG body, corresponding to the known localization of PC2. The antibody has been successfully used to detect FLAG-tagged transfected hPC2 (see figure below). It detects a weak band that probably corresponds to endogenous PC2, however, a strong secondary band is also seen at 50kD in all cell lines thus far tested. This suggests that the antibody may also react with another highly expressed ubiquitous protein.
ELISA: 1:10,000-1:40,000.
Western Blot: 1:1,000-1:4,000.
Immunofluorescence microscopy: 100-1:400.
Background PC2 is the human homolog of the Drosophila 'Polycomb' (Pc) protein which has been identified as a gene family member associated with a cellular memory system that is responsible for the inheritance of gene activity by progeny cells. The human Pc homolog gene is more closely related to a Xenopus Pc homolog, XPc, than to a previously described human Pc homolog, CBX2 (hPc1). However, the hPc2 and CBX2/hPc1 proteins are shown to colocalize in interphase nuclei of human U-2 OS osteosarcoma cells, suggesting that the proteins are part of a common protein complex. The human protein is believed to function as a repressor of proto-oncogene activity and that interference with hPc2 function can lead to derepression of proto-oncogene transcription and subsequently to cellular transformation. Other reports describe PC2 as a protein that has SUMO E3 activity for the corepressors CtBP and CtBP2.
Concentration 0.8 mg/ml (by UV absorbance at 280 nm)
General Readings
  1. Kagey,M.H., Melhuish,T.A. and Wotton,D. (2003) The polycomb protein Pc2 is a SUMO E3. Cell 113 (1), 127- 137.
  2. Satijn DP, Olson DJ, van der Vlag J, Hamer KM, Lambrechts C, Masselink H, et al. Interference with the expression of a novel human polycomb protein, hPc2, results in cellular transformation and apoptosis. Mol Cell Biol. 1997 Oct;17(10):6076-86. PubMed PMID: 9315667. (Free PMC Article available)
  3. Satijn DP, Gunster MJ, van der Vlag J, Hamer KM, Schul W, Alkema MJ, et al. RING1 is associated with the polycomb group protein complex and acts as a transcriptional repressor. Mol Cell Biol. 1997 Jul;17(7):4105-13. PubMed PMID: 9199346. (Free PMC Article available)
Storage Store vial at -20°C prior to opening. Dilute only prior to immediate use.
For extended storage aliquot contents and freeze at -20°C or below.
Avoid cycles of freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Immunoaffinity chromatography.
Buffer System:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, containing 0.01% (w/v) Sodium Azide as preservative.
State:
Liquid (sterile filtred) purified Ig fraction.
Aff - Purified
Specificity
Specificity:
This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase. Reactivity occurs against human PC2 protein. Cross reactivity with PC2 protein from mouse may occur as a 92% homology (BLAST) is noted for the immunized sequence. Cross reactivity with PC2 protein from other sources is not known.

Accessory Products

Proteins and/or Positive Controls

Proteins for CBX4 / PC2 (4 products)

Catalog No. Species Pres. Purity   Source  

CBX4 / PC2

CBX4 / PC2 Human Purified
  Abnova Taiwan Corp.

CBX4 / PC2

CBX4 / PC2 Human Purified
  Abnova Taiwan Corp.

CBX4 / PC2

CBX4 / PC2 Human
  Abnova Taiwan Corp.

CBX4 / PC2

CBX4 / PC2 Human
  Abnova Taiwan Corp.

Positive controls for CBX4 / PC2 (1 products)

Catalog No. Species Pres. Purity   Source  

CBX4 293T Cell Transient Overexpression Lysate(Denatured)

CBX4 293T Cell Transient Overexpression Lysate(Denatured)
  Abnova Taiwan Corp.
  • LinkedIn