AM01039FC-N CD1 antibody

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Mouse anti Bovine, Goat, Sheep CD1 20.27


Product Description for CD1

Mouse anti Bovine, Goat, Sheep CD1 20.27.
Presentation: FITC
Product is tested for Flow Cytometry.

Properties for CD1

Product Category Primary Antibodies
Quantity 0.1 mg
Presentation FITC
Reactivity Bov, Gt, Sh
Applications F
Clonality Monoclonal
Clone 20.27
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Ovine thymocytes.
Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS-1 myeloma cell line.
Application Flow Cytometry: Use 10 µl of 1/5-1/10 diluted CD1 antibody to label 1 x 10e6 cells in 100 µl.
Background All CD1 molecules, except CD1e, are cell surface glycoproteins that are structurally related to the MHC molecules, however, in distinction, CD1 proteins are essentially non polymorphic. CD1 has considerable structural homology with both MHC class I and class II molecules, and CD1 molecules are involved in T cell activation. In contrast to MHC, however, CD1 molecules appear to present predominantly non peptide molecules originating from lipids and glycolipids.
CD1 is primarily expressed in cortical thymocytes and dendritic cells in the dermis, afferent lymph, lymph node, spleen and thymus.
Concentration 0.1 mg/ml
General Readings
  1. Mackay CR, Maddox JF, Gogolin-Ewens KJ, Brandon MR. Characterization of two sheep lymphocyte differentiation antigens, SBU-T1 and SBU-T6. Immunology. 1985 Aug;55(4):729-37. PubMed PMID: 3894224. (Free PMC Article available)
  2. Mackay CR, Maddox JF, Brandon MR. Thymocyte subpopulations during early fetal development in sheep. J Immunol. 1986 Mar 1;136(5):1592-9. PubMed PMID: 2419408.
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
This product is photosensitive and should be protected from light.
Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf life: one year from despatch.
Affinity Chromatography on Protein G.
Buffer System:
PBS, pH 7.4 containing 0.09% Sodium Azide as preservative and 1% BSA as stabilizer.
Liquid purified Ig fraction.
This antibody recognises the ovine CD1 cell surface antigen.
Clone 20.27 does not bind to Granulocytes, Platelets and Erythrocytes.
Sheep, Bovine, Goat.

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