AM05859PU-N CD11R1 antibody

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0.1 mg / €330.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

Quick Overview

Mouse anti Guinea Pig, Human, Porcine CD11R1 MIL4

Product Description for CD11R1

Mouse anti Guinea Pig, Human, Porcine CD11R1 MIL4.
Presentation: Purified
Product is tested for Frozen Sections, Immunoprecipitation, Flow Cytometry.

Properties for CD11R1

Product Category Primary Antibodies
Quantity 0.1 mg
Presentation Purified
Reactivity GP, Hu, Por
Applications C, F, IP
Clonality Monoclonal
Clone MIL4
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Porcine Lamina Propria Leucocytes
Isotype control AM03095PU-N, SM10P (for use in human samples)
Application Flow Cytometry: Use 10 µl of 1/25-1/200 diluted antibody to label 1x106 cells in 100 µl.
Immunoprecipitation.
Immunohistochemistry on Frozen Sections.
General Readings
  1. Haverson K, Bailey M, Higgins VR, Bland PW, Stokes CR. Characterization of monoclonal antibodies specific for monocytes, macrophages and granulocytes from porcine peripheral blood and mucosal tissues. J Immunol Methods. 1994 Apr 15;170(2):233-45. PubMed PMID: 8158001.
  2. Domínguez J, Alvarez B, Alonso F, Thacker E, Haverson K, McCullough K, et al. Workshop studies on monoclonal antibodies in the myeloid panel with CD11 specificity. Vet Immunol Immunopathol. 2001 Jul 20;80(1-2):111-9. PubMed PMID: 11445222.
  3. Inman CF, Singha S, Lewis M, Bradley B, Stokes C, Bailey M. Dendritic cells interact with CD4 T cells in intestinal mucosa. J Leukoc Biol. 2010 Sep;88(3):571-8. doi: 10.1189/jlb.0310161. Epub 2010 Jun 15. PubMed PMID: 20551210.
  4. Cheng Q, Xu C, Zhang L, Li J, Cao T, Zhang M. Administered CpG oligodeoxynucleotide induces mRNA expression of CXC and CC chemokines at the intestinal mucosa and PBMCs in piglets. Int Immunopharmacol. 2010 May;10(5):611-8. doi: 10.1016/j.intimp.2010.02.013. Epub 2010 Mar 2. PubMed PMID: 20202485.
  5. Ordway D, Palanisamy G, Henao-Tamayo M, Smith EE, Shanley C, Orme IM, et al. The cellular immune response to Mycobacterium tuberculosis infection in the guinea pig. J Immunol. 2007 Aug 15;179(4):2532-41. PubMed PMID: 17675515.
  6. Shang S, Shanley CA, Caraway ML, Orme EA, Henao-Tamayo M, Hascall-Dove L, et al. Activities of TMC207, rifampin, and pyrazinamide against Mycobacterium tuberculosis infection in guinea pigs. Antimicrob Agents Chemother. 2011 Jan;55(1):124-31. doi: 10.1128/AAC.00978-10. Epub 2010 Oct 11. PubMed PMID: 20937788. (Free PMC Article available, 5 images available)
  7. Rank RG, Bowlin AK, Tormanen KI, Wang Y, Maurelli AT. Effect of inflammatory response on in vivo competition between two chlamydial variants in the guinea pig model of inclusion conjunctivitis. Infect Immun. 2012 Feb;80(2):612-9. doi: 10.1128/IAI.06054-11. Epub 2011 Dec 5. PubMed PMID: 22144478. (Free PMC Article available, 5 images available)
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Affinity Chromatography on Protein G
Buffer System:
PBS
Preservatives:
0.09% Sodium Azide
Purity:
Tissue Culture Supernatant containing 0.2M Tris/HCl pH7.4 and 5-10% foetal calf serum
State:
Liquid purified IgG fraction from Tissue Culture Supernatant
Purified
Specificity
Specificity:
This antibody recognizes the cell surface antigen classified as CD11R1 and stains Porcine eosinophils, a subset of neutrophils and NK cells. The CD11R1 antigen is a 165kD molecule, identical in size to Human CD11b. However, the expression pattern of CD11b in the Human and CD11R1 in the pig are different.
Clone MIL4 stains porcine eosinophils, a subset of neutrophils and NK cells, it does not stain monocytes or macrophages (Haverson et al. 1994).
Mouse anti Pig CD11R1, clone MIL4 immunoprecipitates a band corresponding to integrin β2 (CD18) of 95kDa, in common with all other anti CD11 antibodies tested at the workshop and also a band of 165kDa corresponding to CD11R1, in a manner identical to the cross reactive anti human CD11b clone, TMG6-5 from peripheral blood mononuclear cell lysates suggesting that porcine CD11R1 is analogous to human CD11b (Dominguez et al. 2001). 
Mouse anti pig CD11R1, clone MIL4 is cross reactive with the guinea pig and is useful for the identification of a population of guinea pig natural killer cells, Kurloff cells (Takizawa et al. 2004) (Eremin et al. 1980).
Species:
Human, Pig, Guinea Pig.

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