DDX0300A546-100 CD123 / IL3RA antibody

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0.1 mg / €360.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

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Mouse anti Canine, Human CD123 / IL3RA 107D2.08

DDX0300A546-100

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Product Description for CD123 / IL3RA

Mouse anti Canine, Human CD123 / IL3RA 107D2.08.
Presentation: Alexa Fluor 546
Product is tested for Immunocytochemistry/Immunofluorescence.

Properties for CD123 / IL3RA

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms IL-3R-alpha, IL3 receptor alpha, Interleukin-3 receptor subunit alpha
Presentation Alexa Fluor 546
Reactivity Can, Hu
Applications ICC/IF
Clonality Monoclonal
Clone 107D2.08
Host Mouse
Isotype IgG1
Shipping to Germany, USA/Canada
PDF datasheet View Datasheet
Manufacturer Dendritics SAS

Datasheet Extract

Immunogen
Swiss Prot Num:
P26951
Immunogen:
sorted human tonsillar pDC
GeneID:
3563
Application DDX0300P-50 DDX0300P-100 Purified: FACS surface, ImmunoHistoChemistry frozen sections, ImmunoHistoChemistry paraffin sections, Immunoprecipitation.
DDX0300A488-50 DDX0300A488-100 AlexaFluor®488: FACS surface, Immunofluorescence.
DDX0300A546-50 DDX0300A546-100 AlexaFluor®546: Immunofluorescence.
DDX0300A647-50 DDX0300A647-100 AlexaFluor®647: Surface and Intracyto Flow cytometry
DDX0300B-50 DDX0300B-100 Biotin: Immunoprecipitation, ImmunoHistoChemistry paraffin sections, ImmunoHistoChemistry frozen sections. 

Usage recommendation:
*This monoclonal antibody may be used between 5-20 μg/ml.
*Optimal dilution should be determined by each laboratory for each application.
*Coupled antibody: to maintain RT before use.
Background IL3 exerts its biologic activity through its interaction with a cell surface receptor that consists of two subunits. The α subunit (CD123) specifically binds IL3, whereas the b subunit is required for signaling and is common to the GMCSFR and IL5-R. 107D2.08 and 106C2.02 mAbs were obtained after mouse immunization with sorted human tonsillar PDC. Both clones strongly stain PDCs and basophils, weakly stain monocytes, CD34+ derived DCs and CD11c+ DC, while no staining is observed on T, B, NK cells as well as on mono-derived DCs. Staining with 107D2.08 and 106C2.02 mAbs are maintained on sorted PDC cultured in the presence of IL3 and CD40L, but lost when IL3 alone is added to the culture. The recognition of the IL3Ra chain by 107D2.08 and 106C2.02 was confirmed by transfection studies. 107D2.08 appeared to be the most appropriate clone for in situ studies. 107D2.08 allowed the first observation of IL3Ra+ cells in breast tumor microenvironment. (Bendriss-Vermare N thesis, 2001;Treilleux I et al, 2004 ; Clin. Canc. Res., 10: 7466-7474).
Concentration 0.5 mg/ml
Product Citations
Originator or purchased from resellers:
  1. Treilleux I, Blay JY, Bendriss-Vermare N, Ray-Coquard I, Bachelot T, Guastalla JP, et al. Dendritic cell infiltration and prognosis of early stage breast cancer. Clin Cancer Res. 2004 Nov 15;10(22):7466-74. PubMed PMID: 15569976.
  2. Tsoumakidou M, Tzanakis N, Papadaki HA, Koutala H, Siafakas NM. Isolation of myeloid and plasmacytoid dendritic cells from human bronchoalveolar lavage fluid. Immunol Cell Biol. 2006 Jun;84(3):267-73. PubMed PMID: 16509829.
  3. van de Laar L, van den Bosch A, van der Kooij SW, Janssen HL, Coffer PJ, van Kooten C, et al. A nonredundant role for canonical NF-κB in human myeloid dendritic cell development and function. J Immunol. 2010 Dec 15;185(12):7252-61. doi: 10.4049/jimmunol.1000672. Epub 2010 Nov 12. PubMed PMID: 21076069.
  4. Bachmann M, Horn K, Rudloff I, Goren I, Holdener M, Christen U, et al. Early production of IL-22 but not IL-17 by peripheral blood mononuclear cells exposed to live Borrelia burgdorferi: the role of monocytes and interleukin-1. PLoS Pathog. 2010 Oct 14;6(10):e1001144. doi: 10.1371/journal.ppat.1001144. PubMed PMID: 20976193. (Free PMC Article available, 8 images available)
  5. Matsuo T, Ichimura K, Tanaka T, Morizane S, Iwatsuki K, Eguchi M, et al. Bilateral conjunctival lesions in blastic plasmacytoid dendritic cell neoplasm. J Clin Exp Hematop. 2011;51(1):49-55. PubMed PMID: 21628861.
  6. Rochereau N, Verrier B, Pin JJ, Genin C, Paul S. Phenotypic localization of distinct DC subsets in mouse Peyer Patch. Vaccine. 2011 May 9;29(20):3655-61. doi: 10.1016/j.vaccine.2011.03.012. Epub 2011 Mar 23. PubMed PMID: 21439318.
  7. Brehm C, Huenecke S, Quaiser A, Esser R, Bremm M, Kloess S, et al. IL-2 stimulated but not unstimulated NK cells induce selective disappearance of peripheral blood cells: concomitant results to a phase I/II study. PLoS One. 2011;6(11):e27351. doi: 10.1371/journal.pone.0027351. Epub 2011 Nov 9. PubMed PMID: 22096557. (Free PMC Article available, 3 images available)
  8. Koehl U, Brehm C, Huenecke S, Zimmermann SY, Kloess S, Bremm M, et al. Clinical grade purification and expansion of NK cell products for an optimized manufacturing protocol. Front Oncol. 2013 May 17;3:118. doi: 10.3389/fonc.2013.00118. eCollection 2013. PubMed PMID: 23730623. (Free PMC Article available, 5 images available)
  9. Broughton SE, Hercus TR, Hardy MP, McClure BJ, Nero TL, Dottore M, et al. Dual mechanism of interleukin-3 receptor blockade by an anti-cancer antibody. Cell Rep. 2014 Jul 24;8(2):410-9. doi: 10.1016/j.celrep.2014.06.038. Epub 2014 Jul 17. PubMed PMID: 25043189.
Storage -20°C. KEEP CONTENTS STERILE: no preservative.
Purified antibodies: avoid repeated freeze/thaw cycles. 
Coupled antibodies: glycerol protects from freezing.
Format
Label:
Alexa Fluor 546
Purification:
QMA Hyper D ion exchange chromatography
Buffer System:
Purified: 100 μg in 200 μl / 50 μg in 100 μl Tris-NaCl pH 8.
Coupled: 100 μg in 200μl / 50 μg in 100 μl PBS 50% glycerol.
Specificity
Specificity:
Human CD123 (IL3Ra). 
Species cross-reactivity: Dog.

Accessory Products

Proteins and/or Positive Controls

Proteins for CD123 / IL3RA (5 products)

Catalog No. Species Pres. Purity   Source  

CD123 / IL3RA

CD123 / IL3RA Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

CD123 / IL3RA

CD123 / IL3RA Human
  Abnova Taiwan Corp.

CD123 / IL3RA

CD123 / IL3RA Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

CD123 / IL3RA

CD123 / IL3RA Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

CD123 / IL3RA

Western Blot: IL3RA Protein [NBP1-46082] - Lane 1  – MW markers;  Lane 2  –  IL-3 R alpha-Fc  Chimera;   Lane 3 - IL-3 R alpha-Fc  Chimera treated with PNGase F to remove potential N-linked glycans; Lane 4 – IL-3 R alpha-Fc Chimera treated with a glycosidase cocktail to remove potential N- and O-linked glycans. 10 µg protein loaded per lane; Deep Purple™ stained. Drop in MW after treatment with PNGase F indicates presence of N-linked glycans. Subsequent drop in MW after treatment with glycosidase cocktail indicates presence of O-linked glycans. Faint bands in lane 3 and lane 4 are glycosidase enzymes. Human Purified
  Novus Biologicals Inc.

Positive controls for CD123 / IL3RA (2 products)

Catalog No. Species Pres. Purity   Source  

IL3RA Lysate

Western Blot: IL3RA Lysate [NBL1-11956] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for IL3RA
  Novus Biologicals Inc.

IL3RA overexpression lysate

IL3RA overexpression lysate
0.1 mg / €295.00
  OriGene Technologies, Inc.
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