AM08137PU-N CD146 antibody

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0.5 mg / €340.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

Quick Overview

Mouse anti Chicken CD146 c264

Product Description for CD146

Mouse anti Chicken CD146 c264.
Presentation: Purified
Product is tested for Frozen Sections, Immunoprecipitation, Flow Cytometry.

Properties for CD146

Product Category Primary Antibodies
Target Category
Quantity 0.5 mg
Synonyms A32, CD146, Cell surface glycoprotein P1H12, MCAM, MUC18, Melanoma cell adhesion molecule, Melanoma-associated antigen MUC18, S-endo 1 endothelial-associated antigen
Presentation Purified
Reactivity Chk
Applications C, F, IP
Clonality Monoclonal
Clone c264
Host Mouse
Isotype IgG2b
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P43121
Immunogen:
Pooled Ed15 and Ed16 thymocytes from H.B15, H.B19 and H.B21 chicken embryos.
GeneID:
4162
Isotype control AM03110PU-N, SM12P
Application Flow Cytometry: < / = 1 µg/10e6 cells. (Ref.1)
Immunoprecipitation. (Ref.1)
Immunohistochemistry (Acetone-Fixed, Frozen Tissue Sections). (Ref.2)
Background CD146 belongs to the immunoglobulin supergene family with five immunoglobulin like domains (V-V-C2-C2-C2), a transmembrane region and a 63 residue cytoplasmic tail. The protein is a membrane glycoprotein that functions as a Ca2+ independent cell adhesion molecule involved in heterophilic cell to cell interactions. CD146 has a molecular size of 130 kDa in its reduced form (118 kDa unreduced), and N linked glycosylation accounts for fifty percent of the apparent molecular weight. In some cells the molecule carries a sulfate 3 glucuronyl moiety.
Expression of the molecule was shown in a relatively limited spectrum of normal human tissues (endothelium, smooth muscle, and subpopulations of activated T cells) and malignant neoplasm (melanoma and breast carcinoma). The lineage specific expression pattern of CD146 can be useful in the differential diagnosis of certain lesions including melanomas and various types of gestational trophoblastic lesions. CD146 expression can promote tumor progression in human melanoma, through enhanced interaction between melanoma cells and endothelial cells. However, in breast carcinoma, CD146 may act as a tumor suppressor. Overexpression of CD146 in breast carcinoma cells results in a more cohesive cell growth and in the formation of smaller tumors in nude mice. During implantation and placentation, CD146 is expressed by the intermediate trophoblast in the placental site and binds to its putative receptor in uterine smooth muscle cells thus limiting trophoblastic invasion in the myometrium. Monoclonal antibody specific for CD146 is an important tool for the identification and isolation of cells expressing CD146.
Concentration 0.5 mg/ml
General Readings 1. Vainio, O., D. Dunon, F. Aissi, J.-P. Dangy, K.M. McNagny, and B.A. Imhof. 1996. HEMCAM, an adhesion molecules
expressed by c-kit+ hemopoietic progenitors. J. Cell. Biol. 135:1655-1668.
2. Vainio, O. 1998. Personal communication.
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Buffer System:
100 mM Borate Buffered Saline, pH 8.2.
No preservatives or amine-containing buffer salts added.
State:
Liquid purified Ig fraction.
Purified
Specificity
Specificity:
This antibody recognizes Chicken MCAM, Mr 98 kDa (protein core ~84 kDa).
Species:
Chicken.
Gene ID 4162

Accessory Products

Proteins and/or Positive Controls

Proteins for CD146 (10 products)

Catalog No. Species Pres. Purity   Source  

CD146

CD146 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

CD146

CD146 Human Purified E. coli
  OriGene Technologies GmbH

CD146

CD146 Human Purified E. coli
  OriGene Technologies GmbH

CD146

CD146 Human Purified E. coli
  OriGene Technologies GmbH

CD146

CD146 Human
  Abnova Taiwan Corp.

CD146

CD146 Human
  Abnova Taiwan Corp.

CD146

CD146 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

CD146

CD146 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

CD146

CD146 structure and sequence Human Purified E. coli
2 µg / €310.00
  Neuromics Antibodies

CD146

CD146 structure and sequence Human Purified E. coli
5 µg / €440.00
  Neuromics Antibodies

Positive controls for CD146 (1 products)

Catalog No. Species Pres. Purity   Source  

MCAM 293T Cell Transient Overexpression Lysate(Denatured)

MCAM 293T Cell Transient Overexpression Lysate(Denatured)
  Abnova Taiwan Corp.
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