SM1860F CD146 antibody

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0.1 mg / €530.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

Quick Overview

Mouse anti Human, Porcine CD146 OJ79c

SM1860F

Product Description for CD146

Mouse anti Human, Porcine CD146 OJ79c.
Presentation: FITC
Product is tested for Frozen Sections, Enzyme Immunoassay, Flow Cytometry.

Properties for CD146

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms A32, CD146, Cell surface glycoprotein P1H12, MCAM, MUC18, Melanoma cell adhesion molecule, Melanoma-associated antigen MUC18, S-endo 1 endothelial-associated antigen
Presentation FITC
Reactivity Hu, Por
Applications C, E, F
Clonality Monoclonal
Clone OJ79c
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P43121
Immunogen:
Recombinant Human MUC18 (D1-D5) Fc protein.
Spleen cells from immunised mice were fused with cells of the mouse Sp2/0 Ag.14 myeloma cell line.
GeneID:
4162
Isotype control SM10F (for use in human samples)
Application Flow Cytometry: Use 10 µl of neat-1/10 diluted CD146 antibody to label 106 cells in 100 µl.
Clone OJ79c (Purified antibody Cat.-No. SM1860P/PT) has been successfully used in ELISA and Immunohistochemistry on Frozen Sections.
Background CD146 is a member of the immunoglobulin superfamily, expressed by all endothelial cells and by melanoma cells. CD146 appears to act as an adhesion molecule. Expression in melanoma may be linked to disease progression.
Concentration 0.1 mg/ml
Product Citations
Purchased from Acris:
  1. Möbius-Winkler S, Hilberg T, Menzel K, Golla E, Burman A, Schuler G, et al. Time-dependent mobilization of circulating progenitor cells during strenuous exercise in healthy individuals. J Appl Physiol (1985). 2009 Dec;107(6):1943-50. doi: 10.1152/japplphysiol.00532.2009. Epub 2009 Oct 1. PubMed PMID: 19797690.
General Readings
  1. Kuzu I, Bicknell R, Fletcher CD, Gatter KC. Expression of adhesion molecules on the endothelium of normal tissue vessels and vascular tumors. Lab Invest. 1993 Sep;69(3):322-8. PubMed PMID: 7690867.
  2. Crisan M, Yap S, Casteilla L, Chen CW, Corselli M, Park TS, et al. A perivascular origin for mesenchymal stem cells in multiple human organs. Cell Stem Cell. 2008 Sep 11;3(3):301-13. doi: 10.1016/j.stem.2008.07.003. PubMed PMID: 18786417.
  3. Iohara K, Zheng L, Wake H, Ito M, Nabekura J, Wakita H, et al. A novel stem cell source for vasculogenesis in ischemia: subfraction of side population cells from dental pulp. Stem Cells. 2008 Sep;26(9):2408-18. doi: 10.1634/stemcells.2008-0393. Epub 2008 Jun 26. PubMed PMID: 18583536.
  4. Park, T.S. et al. (2010) Placental Perivascular Cells for Human Muscle Regeneration. Stem Cells Dev. Oct 5. [Epub ahead of print].
  5. Smith K, Malatesti N, Cauchon N, Hunting D, Lecomte R, van Lier JE, et al. Mono- and tri-cationic porphyrin-monoclonal antibody conjugates: photodynamic activity and mechanism of action. Immunology. 2011 Feb;132(2):256-65. doi: 10.1111/j.1365-2567.2010.03359.x. Epub 2010 Oct 29. PubMed PMID: 21039468. (Free PMC Article available, 7 images available)
  6. James AW, Zara JN, Zhang X, Askarinam A, Goyal R, Chiang M, et al. Perivascular stem cells: a prospectively purified mesenchymal stem cell population for bone tissue engineering. Stem Cells Transl Med. 2012 Jun;1(6):510-9. doi: 10.5966/sctm.2012-0002. Epub 2012 Jun 11. PubMed PMID: 23197855. (Free PMC Article available, 6 images available)
  7. Wassmer SC, Moxon CA, Taylor T, Grau GE, Molyneux ME, Craig AG. Vascular endothelial cells cultured from patients with cerebral or uncomplicated malaria exhibit differential reactivity to TNF. Cell Microbiol. 2011 Feb;13(2):198-209. doi: 10.1111/j.1462-5822.2010.01528.x. Epub 2010 Oct 7. PubMed PMID: 21029292. (Free PMC Article available, 6 images available)
  8. Lee, J.H. et al. (2012) Generation of osteogenic construct using periosteal-derived osteoblasts and polydioxanone/pluronic F127 scaffold with periosteal-derived CD146 positive endothelial-like cells. Journal of Biomedical Materials Research Part A. Sep 8. [Epub ahead of print]
  9. Boneberg EM, Illges H, Legler DF, Fürstenberger G. Soluble CD146 is generated by ectodomain shedding of membrane CD146 in a calcium-induced, matrix metalloprotease-dependent process. Microvasc Res. 2009 Dec;78(3):325-31. doi: 10.1016/j.mvr.2009.06.012. Epub 2009 Jul 15. PubMed PMID: 19615385.
  10. Murakami M, Imabayashi K, Watanabe A, Takeuchi N, Ishizaka R, Iohara K, et al. Identification of novel function of vimentin for quality standard for regenerated pulp tissue. J Endod. 2012 Jul;38(7):920-6. doi: 10.1016/j.joen.2012.01.010. Epub 2012 Mar 15. PubMed PMID: 22703654.
  11. Nielsen CT, Østergaard O, Johnsen C, Jacobsen S, Heegaard NH. Distinct features of circulating microparticles and their relationship to clinical manifestations in systemic lupus erythematosus. Arthritis Rheum. 2011 Oct;63(10):3067-77. doi: 10.1002/art.30499. PubMed PMID: 21702008.
  12. Zhang Y, Fisher N, Newey SE, Smythe J, Tatton L, Tsaknakis G, et al. The impact of proliferative potential of umbilical cord-derived endothelial progenitor cells and hypoxia on vascular tubule formation in vitro. Stem Cells Dev. 2009 Mar;18(2):359-75. doi: 10.1089/scd.2008.0071. PubMed PMID: 18662129.
  13. Iversen LV, Ostergaard O, Nielsen CT, Jacobsen S, Heegaard NH. A heparin-based method for flow cytometric analysis of microparticles directly from platelet-poor plasma in calcium containing buffer. J Immunol Methods. 2013 Feb 28;388(1-2):49-59. doi: 10.1016/j.jim.2012.12.001. Epub 2012 Dec 12. PubMed PMID: 23246793.
  14. Meireles AL, Hallack Neto AE, Costa Rde O, Pereira J. Increased levels of circulating endothelial progenitor cells in human T-cell lymphotropic virus type I carriers. Arch Med Res. 2011 Jan;42(1):34-7. doi: 10.1016/j.arcmed.2011.01.002. PubMed PMID: 21376260.
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Label:
Fluorescein Isothiocyanate Isomer 1
FITC
Purification:
Affinity Chromatography on Protein G
Buffer System:
PBS
Preservatives:
0.09% Sodium Azide
Stabilizers:
1% BSA
State:
Liquid purified IgG fraction.
Specificity
Specificity:
This antibody recognises the 118 kD cell surface glycoprotein CD146, also known as MUC18, Mel-CAM and S-endo. CD146 is a member of the immunoglobulin superfamily, expressed by all endothelial cells and by melanoma cells.
Species:
Human and Pig.
Gene ID 4162

Accessory Products

Proteins and/or Positive Controls

Proteins for CD146 (10 products)

Catalog No. Species Pres. Purity   Source  

CD146

CD146 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

CD146

CD146 Human Purified E. coli
  Acris Antibodies GmbH

CD146

CD146 Human Purified E. coli
  Acris Antibodies GmbH

CD146

CD146 Human Purified E. coli
  Acris Antibodies GmbH

CD146

CD146 Human
  Abnova Taiwan Corp.

CD146

CD146 Human
  Abnova Taiwan Corp.

CD146

CD146 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

CD146

CD146 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

CD146

CD146 structure and sequence Human Purified E. coli
2 µg / €310.00
  Neuromics Antibodies

CD146

CD146 structure and sequence Human Purified E. coli
5 µg / €440.00
  Neuromics Antibodies

Positive controls for CD146 (1 products)

Catalog No. Species Pres. Purity   Source  

MCAM 293T Cell Transient Overexpression Lysate(Denatured)

MCAM 293T Cell Transient Overexpression Lysate(Denatured)
  Abnova Taiwan Corp.
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