SM1860PS CD146 antibody

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0.1 mg / €320.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

Quick Overview

Rat anti Human CD146 OJ79c

SM1860PS

Product Description for CD146

Rat anti Human CD146 OJ79c.
Presentation: Purified
Product is tested for Western blot / Immunoblot, Immunoprecipitation, Immunocytochemistry/Immunofluorescence, Flow Cytometry, Frozen Sections.

Properties for CD146

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms A32, CD146, Cell surface glycoprotein P1H12, MCAM, MUC18, Melanoma cell adhesion molecule, Melanoma-associated antigen MUC18, S-endo 1 endothelial-associated antigen
Presentation Purified
Reactivity Hu
Applications C, F, ICC/IF, IP, WB
Clonality Monoclonal
Clone OJ79c
Host Rat
Isotype IgG1
Molecular weight 130 kDa
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P43121
Immunogen:
Recombinant Human MUC18  protein.
GeneID:
4162
Isotype control SM25P, SM25PX
Application ELISA: Use BSA free antibody for coating. 
Flow Cytometry: 0.5-1 µg/106 cells. 
Immunofluorescence: 0.5-1 µg/ml.
Western Blot: 0.5-1 µg/ml. 
Immunoprecipitation: 1-2 μg/500 µg protein lysate.
Immunohistochemistry on Frozen Sections: 0.5-1 µg/ml for 30 minutes at RT.
Positive Control: A-375, HUV-EC or HeLa Cells, Tonsil or Melanoma.
Background The Human Mel-CAM gene maps to chromosome 11q23 and encodes a trans-membrane glycoprotein, also designated MCAM, MUC18 or CD146, that belongs to the immunoglobulin superfamily and functions as a Ca2+-independent cell adhesion molecule. Mel-CAM expression is restricted to advanced primary and metastatic melanomas and to cell lines of the neuroectodermal lineage, but not normal melanocytes. Mel-CAM is found on 80% of advanced primary human melanomas and correlates well with development of metastatic disease.
Concentration 0.2 mg/ml
General Readings
  1. Pruszak J, Sonntag KC, Aung MH, Sanchez-Pernaute R, Isacson O. Markers and methods for cell sorting of human embryonic stem cell-derived neural cell populations. Stem Cells. 2007 Sep;25(9):2257-68. Epub 2007 Jun 21. PubMed PMID: 17588935. (Free PMC Article available, 6 images available)
  2. Paul G, Özen I, Christophersen NS, Reinbothe T, Bengzon J, Visse E, et al. The adult human brain harbors multipotent perivascular mesenchymal stem cells. PLoS One. 2012;7(4):e35577. doi: 10.1371/journal.pone.0035577. Epub 2012 Apr 16. PubMed PMID: 22523602. (Free PMC Article available, 9 images available)
  3. Crisan M, Yap S, Casteilla L, Chen CW, Corselli M, Park TS, et al. A perivascular origin for mesenchymal stem cells in multiple human organs. Cell Stem Cell. 2008 Sep 11;3(3):301-13. doi: 10.1016/j.stem.2008.07.003. PubMed PMID: 18786417.
  4. Iohara K, Zheng L, Wake H, Ito M, Nabekura J, Wakita H, et al. A novel stem cell source for vasculogenesis in ischemia: subfraction of side population cells from dental pulp. Stem Cells. 2008 Sep;26(9):2408-18. doi: 10.1634/stemcells.2008-0393. Epub 2008 Jun 26. PubMed PMID: 18583536.
  5. Park, T.S. et al. (2010) Placental Perivascular Cells for Human Muscle Regeneration. Stem Cells Dev. Oct 5. [Epub ahead of print]
  6. Smith K, Malatesti N, Cauchon N, Hunting D, Lecomte R, van Lier JE, et al. Mono- and tri-cationic porphyrin-monoclonal antibody conjugates: photodynamic activity and mechanism of action. Immunology. 2011 Feb;132(2):256-65. doi: 10.1111/j.1365-2567.2010.03359.x. Epub 2010 Oct 29. PubMed PMID: 21039468. (Free PMC Article available, 7 images available)
  7. James AW, Zara JN, Zhang X, Askarinam A, Goyal R, Chiang M, et al. Perivascular stem cells: a prospectively purified mesenchymal stem cell population for bone tissue engineering. Stem Cells Transl Med. 2012 Jun;1(6):510-9. doi: 10.5966/sctm.2012-0002. Epub 2012 Jun 11. PubMed PMID: 23197855. (Free PMC Article available, 6 images available)
  8. Wassmer SC, Moxon CA, Taylor T, Grau GE, Molyneux ME, Craig AG. Vascular endothelial cells cultured from patients with cerebral or uncomplicated malaria exhibit differential reactivity to TNF. Cell Microbiol. 2011 Feb;13(2):198-209. doi: 10.1111/j.1462-5822.2010.01528.x. Epub 2010 Oct 7. PubMed PMID: 21029292. (Free PMC Article available, 6 images available)
  9. Lee, J.H. et al. (2012) Generation of osteogenic construct using periosteal-derived osteoblasts and polydioxanone/pluronic F127 scaffold with periosteal-derived CD146 positive endothelial-like cells. Journal of Biomedical Materials Research Part A. Sep 8. [Epub ahead of print]
  10. Boneberg EM, Illges H, Legler DF, Fürstenberger G. Soluble CD146 is generated by ectodomain shedding of membrane CD146 in a calcium-induced, matrix metalloprotease-dependent process. Microvasc Res. 2009 Dec;78(3):325-31. doi: 10.1016/j.mvr.2009.06.012. Epub 2009 Jul 15. PubMed PMID: 19615385.
  11. Murakami M, Imabayashi K, Watanabe A, Takeuchi N, Ishizaka R, Iohara K, et al. Identification of novel function of vimentin for quality standard for regenerated pulp tissue. J Endod. 2012 Jul;38(7):920-6. doi: 10.1016/j.joen.2012.01.010. Epub 2012 Mar 15. PubMed PMID: 22703654.
  12. Nielsen CT, Østergaard O, Johnsen C, Jacobsen S, Heegaard NH. Distinct features of circulating microparticles and their relationship to clinical manifestations in systemic lupus erythematosus. Arthritis Rheum. 2011 Oct;63(10):3067-77. doi: 10.1002/art.30499. PubMed PMID: 21702008.
  13. Zhang Y, Fisher N, Newey SE, Smythe J, Tatton L, Tsaknakis G, et al. The impact of proliferative potential of umbilical cord-derived endothelial progenitor cells and hypoxia on vascular tubule formation in vitro. Stem Cells Dev. 2009 Mar;18(2):359-75. doi: 10.1089/scd.2008.0071. PubMed PMID: 18662129.
  14. Iversen, L.V. et al. (2012) A heparin-based method for flow cytometric analysis of microparticles directly from platelet-poor plasma in calcium containing buffer. Journal of Immunological Methods Dec 12 [Epub ahead of print]
  15. Meireles AL, Hallack Neto AE, Costa Rde O, Pereira J. Increased levels of circulating endothelial progenitor cells in human T-cell lymphotropic virus type I carriers. Arch Med Res. 2011 Jan;42(1):34-7. doi: 10.1016/j.arcmed.2011.01.002. PubMed PMID: 21376260.
  16. Iversen, L. et al. (2013) Circulating microparticles and plasma levels of soluble E- and P-selectins in patients with systemic sclerosis. Scand J Rheumatol. Sep 9. [Epub ahead of print]
  17. Murakami, M. et al. (2013) The use of granulocyte-colony stimulating factor induced mobilization for isolation of dental pulp stem cells with high regenerative potential. Biomaterials. pii: S0142-9612(13)00942-3.
  18. Ruetze M, Knauer T, Gallinat S, Wenck H, Achterberg V, Maerz A, et al. A novel niche for skin derived precursors in non-follicular skin. J Dermatol Sci. 2013 Feb;69(2):132-9. doi: 10.1016/j.jdermsci.2012.10.007. Epub 2012 Oct 26. PubMed PMID: 23148930.
  19. Dokić J, Tomić S, Marković M, Milosavljević P, Colić M. Mesenchymal stem cells from periapical lesions modulate differentiation and functional properties of monocyte-derived dendritic cells. Eur J Immunol. 2013 Jul;43(7):1862-72. doi: 10.1002/eji.201243010. Epub 2013 May 28. PubMed PMID: 23616249.
Storage Store undiluted at 2-8°C.
DO NOT FREEZE!
Shelf life: one year from despatch.
Format
Purification:
Protein A/G Chromatography
Buffer System:
10mM PBS
Preservatives:
0.05% Sodium Azide
Stabilizers:
0.05% BSA
State:
Liquid purified IgG fraction from Bioreactor Concentrate
Purified
Species Reactivity
Species reactivity (tested):
Human.
Specificity
Specificity:
This antibody recognises the 118kD cell surface glycoprotein CD146.
Cellular Localization: Cell surface.

Accessory Products

Proteins and/or Positive Controls

Proteins for CD146 (10 products)

Catalog No. Species Pres. Purity   Source  

CD146

CD146 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

CD146

CD146 Human Purified E. coli
  Acris Antibodies GmbH

CD146

CD146 Human Purified E. coli
  Acris Antibodies GmbH

CD146

CD146 Human Purified E. coli
  Acris Antibodies GmbH

CD146

CD146 Human
  Abnova Taiwan Corp.

CD146

CD146 Human
  Abnova Taiwan Corp.

CD146

CD146 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

CD146

CD146 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

CD146

CD146 structure and sequence Human Purified E. coli
2 µg / €310.00
  Neuromics Antibodies

CD146

CD146 structure and sequence Human Purified E. coli
5 µg / €440.00
  Neuromics Antibodies

Positive controls for CD146 (1 products)

Catalog No. Species Pres. Purity   Source  

MCAM 293T Cell Transient Overexpression Lysate(Denatured)

MCAM 293T Cell Transient Overexpression Lysate(Denatured)
  Abnova Taiwan Corp.
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