DDX0141P-100 CD154 / CD40L antibody

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0.1 mg / €440.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

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Mouse anti Human CD154 / CD40L LL2

DDX0141P-100

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  • DDX0141P-100

Product Description for CD154 / CD40L

Mouse anti Human CD154 / CD40L LL2.
Presentation: Purified
Product is tested for Functional assay, Enzyme Immunoassay, Flow Cytometry, Immunocytochemistry/Immunofluorescence.

Properties for CD154 / CD40L

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms CD40 ligand, CD40-L, CD40LG, GP39, T-cell antigen Gp39, TNF-related activation protein, TNFSF5, TRAP, Tumor necrosis factor ligand superfamily member 5
Presentation Purified
Reactivity Hu
Applications E, F, FN, ICC/IF
Clonality Monoclonal
Clone LL2
Host Mouse
Isotype IgG1
Shipping to Germany, USA/Canada
PDF datasheet View Datasheet
Manufacturer Dendritics SAS

Datasheet Extract

Immunogen
Swiss Prot Num:
P29965
Immunogen:
Chimeric Human CD40L-Ig-transfected COS-7 cells.
GeneID:
959
Isotype control AM03095PU-N, SM10P (for use in human samples)
Application Flow cytometry, IF, T cell activation, CD40-CD40L blocking, ELISA for sCD154 detection.
*This monoclonal antibody may be used between 5-20 μg/ml.
Background CD40 ligand also known as TRAP, gp39, TNFSF5, is a 261 amino acid type II transmembrane glycoprotein belonging to the TNF superfamily. CD40 ligand is expressed predominantly on activated CD4+ T cells and is also found on other cell types, including NK cells, mast cells, basophils and eosinophils. Genetic alterations in the CD40L results in the absence of a functional molecule on activated T cells and are responsible for the occurrence of X-linked hyper-IgM syndrome. LL2 clone was produced by immunizing mice with L cells transfected with human CD40L. Addition of anti-CD40L mAb LL2 enhanced proliferation of MT9 and FS35 T cell lines in the presence of IL-2 and increased the secretion of IFN-γ by MT9 cells and of IL-10, IL-5 and IL-4 by FS35 cells cultured with IL-2. LL2 completely blocks CD40- CD40L interaction, can mimic T cell-induced CD40 triggering on B cells and activates T cells.
(Hermann P et al, 1995, EJI, 25 (10), 2972-2977; Razanajaona D et al, 1996, J Immunol., 157(4):1492-8 ; Fournier N et al, 2000 J Immunol., 165: 1197–1209; Henn et al, 2001, Blood, 98 (4), 1047-1054).
Concentration 0.5 mg/ml
Product Citations
Originator or purchased from resellers:
  1. Rissoan MC, Van Kooten C, Chomarat P, Galibert L, Durand I, Thivolet-Bejui F, et al. The functional CD40 antigen of fibroblasts may contribute to the proliferation of rheumatoid synovium. Clin Exp Immunol. 1996 Dec;106(3):481-90. PubMed PMID: 8973616. (Free PMC Article available)
  2. Razanajaona D, van Kooten C, Lebecque S, Bridon JM, Ho S, Smith S, et al. Somatic mutations in human Ig variable genes correlate with a partially functional CD40-ligand in the X-linked hyper-IgM syndrome. J Immunol. 1996 Aug 15;157(4):1492-8. PubMed PMID: 8759730.
  3. Henn V, Steinbach S, Büchner K, Presek P, Kroczek RA. The inflammatory action of CD40 ligand (CD154) expressed on activated human platelets is temporally limited by coexpressed CD40. Blood. 2001 Aug 15;98(4):1047-54. PubMed PMID: 11493450.
Storage -20°C. KEEP CONTENTS STERILE: no preservative.
Purified antibodies: avoid repeated freeze/thaw cycles.
Coupled antibodies: glycerol protects from freezing.
Format
Purification:
QMA Hyper D ion exchange chromatography
Buffer System:
100 μg in 200 μl / 50 μg in 100 μl Tris-NaCl pH 8
State:
Purified
Specificity
Specificity:
human CD40L.

Accessory Products

Proteins and/or Positive Controls

Proteins for CD154 / CD40L (25 products)

Catalog No. Species Pres. Purity   Source  

CD154 / CD40L

CD154 / CD40L Human > 95 %
Preparation: .
Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
10 µg / €299.00
  OriGene Technologies, Inc.

CD154 / CD40L

CD154 / CD40L Human > 95 %
Preparation: .
Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
50 µg / €139.00
  OriGene Technologies, Inc.

CD154 / CD40L

CD154 / CD40L Human > 95 %
Preparation: .
Purity Detail: >95%, as determined by Coomassie stained SDS-PAGE.
HEK293 cells
10 µg / €99.00
  OriGene Technologies, Inc.

CD154 / CD40L (113-261, His-tag)

CD154 / CD40L Human Purified > 95 % E. coli
0.5 mg / €750.00
  OriGene Technologies GmbH

CD154 / CD40L (113-261, His-tag)

CD154 / CD40L Human Purified > 95 % E. coli
0.1 mg / €300.00
  OriGene Technologies GmbH

CD154 / CD40L

CD154 / CD40L Human Purified > 98 % pure by SDS-PAGE and HPLC analyses E. coli
  OriGene Technologies GmbH

CD154 / CD40L

CD154 / CD40L Human Purified > 98 % pure by SDS-PAGE and HPLC analyses E. coli
  OriGene Technologies GmbH

CD154 / CD40L

CD154 / CD40L Human Purified > 98 % Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.

Amino acid sequence: The sequence of the first five N-terminal amino acids was determined a
E. coli
  OriGene Technologies GmbH

CD154 / CD40L

CD154 / CD40L Human Purified > 98 % Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.

Amino acid sequence: The sequence of the first five N-terminal amino acids was determined a
E. coli
  OriGene Technologies GmbH

CD154 / CD40L

CD154 / CD40L Human Purified > 98 % Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.

Amino acid sequence: The sequence of the first five N-terminal amino acids was determined a
E. coli
  OriGene Technologies GmbH

CD154 / CD40L

CD154 / CD40L Human
  Abnova Taiwan Corp.

CD154 / CD40L

CD154 / CD40L Human Human HEK293T cells
  Abnova Taiwan Corp.

CD154 / CD40L

CD154 / CD40L Human 12.5% SDS-PAGE Stained with Coomassie Blue
  Abnova Taiwan Corp.

CD154 / CD40L

CD154 / CD40L Human 12.5% SDS-PAGE Stained with Coomassie Blue
  Abnova Taiwan Corp.

CD154 / CD40L

CD154 / CD40L Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

CD154 / CD40L

CD154 / CD40L Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

CD154 / CD40L

CD154 / CD40L Human
  Abnova Taiwan Corp.

CD154 / CD40L

CD154 / CD40L Human
  Abnova Taiwan Corp.

CD154 / CD40L

CD40 Ligand/TRAP Structure and Sequence Human Purified > 98 % E. coli
10 µg / €310.00
  Neuromics Antibodies

CD154 / CD40L

CD154 / CD40L Human Purified > 98 % E. coli
  Neuromics Antibodies

CD154 / CD40L

CD154 / CD40L Human Purified
  Novus Biologicals Inc.

CD154 / CD40L

CD154 / CD40L Human Purified
  Novus Biologicals Inc.

CD154 / CD40L

CD154 / CD40L Human Purified
  Novus Biologicals Inc.

CD154 / CD40L

CD154 / CD40L Human Purified
  Novus Biologicals Inc.

CD154 / CD40L

CD154 / CD40L Human Purified
  Novus Biologicals Inc.

Positive controls for CD154 / CD40L (2 products)

Catalog No. Species Pres. Purity   Source  

CD40LG overexpression lysate

CD40LG overexpression lysate
0.1 mg / €295.00
  OriGene Technologies, Inc.

CD40L Lysate

Western Blot: CD40L Lysate [NBL1-08949] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for CD40LG
  Novus Biologicals Inc.
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