discontinued

SM2036RT CD158 / KIR2D antibody

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Mouse anti Human CD158 / KIR2D NKVFS1

Product Description for CD158 / KIR2D

Mouse anti Human CD158 / KIR2D NKVFS1.
Presentation: PE
Product is tested for Flow Cytometry.

Properties for CD158 / KIR2D

Product Category Primary Antibodies
Target Category
Quantity 25 Tests
Synonyms CD158a, CD158b, CD158i, KI2DL1, KILLER CELL Immunoglobin-Like Receptors, KIR2DL3, KIR2DS4
Presentation PE
Reactivity Hu
Applications F
Clonality Monoclonal
Clone NKVFS1
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Isotype control SM10R (for use in human samples)
Application Flow Cytometry: Use 10 µl of neat antibody to label 10e6 cells in 100 µl.
Background KIR2D family members are cell surface glycoproteins with two Ig domains, which are expressed on natural killer cells and some T cells.
General Readings
  1. Spaggiari GM, Contini P, Carosio R, Arvigo M, Ghio M, Oddone D, et al. Soluble HLA class I molecules induce natural killer cell apoptosis through the engagement of CD8: evidence for a negative regulation exerted by members of the inhibitory receptor superfamily. Blood. 2002 Mar 1;99(5):1706-14. PubMed PMID: 11861287.
  2. Spaggiari GM, Contini P, Dondero A, Carosio R, Puppo F, Indiveri F, et al. Soluble HLA class I induces NK cell apoptosis upon the engagement of killer-activating HLA class I receptors through FasL-Fas interaction. Blood. 2002 Dec 1;100(12):4098-107. Epub 2002 Jul 18. PubMed PMID: 12393468.
  3. Older Aguilar AM, Guethlein LA, Adams EJ, Abi-Rached L, Moesta AK, Parham P. Coevolution of killer cell Ig-like receptors with HLA-C to become the major variable regulators of human NK cells. J Immunol. 2010 Oct 1;185(7):4238-51. doi: 10.4049/jimmunol.1001494. Epub 2010 Aug 30. PubMed PMID: 20805421. (Free PMC Article available, 9 images available)
Storage Prior to and following reconstitution store the antibody at 2-8°C.
DO NOT FREEZE!
This product is photosensitive and should be protected from light.
Shelf life: one year from despatch.
Format
Label:
R. Phycoerythrin (RPE)
PE
Purification:
Affinity Chromatography on Protein G
Buffer System:
PBS, pH 7.4 containing 0.09% Sodium Azide, 5% Sucrose and 1% BSA
State:
Lyophilized purified IgG fraction.
Reconstitution:
SM2036R: Restore with 1 ml distilled water.
SM2036RT: Restore with 0.25 ml distilled water.
Specificity
Specificity:
This antibody recognizes KIR2D members of the killer cell immunoglobulin (Ig)-like receptor (KIR) family, CD158a, CD158b and P50.3.
Clone NKVFS1 specifically recognizes the long and short forms CD158a and CD158b (KIR2DL, KIR2DS1 and KIR2DS2 respectively) and also p50.3 (KIR2DS4). The clone is reported to have functional activity, activating NK cell cytotoxicity via KIR2DS and inhibiting via KIR2DL forms. We recommend the use of SM2036A for this purpose.
Species:
Human.
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