SM1229P CD158b / KIR2DL3 antibody

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0.2 mg / €570.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

Quick Overview

Mouse anti Human CD158b / KIR2DL3 GL183

SM1229P

Product Description for CD158b / KIR2DL3

Mouse anti Human CD158b / KIR2DL3 GL183.
Presentation: Purified
Product is tested for Immunoprecipitation, Western blot / Immunoblot, Flow Cytometry.

Properties for CD158b / KIR2DL3

Product Category Primary Antibodies
Target Category
Quantity 0.2 mg
Synonyms KIRCL23, Killer cell immunoglobulin-like receptor 2DL3, MHC class I NK cell receptor, NKAT2a, NKAT2b, Natural killer-associated transcript 2
Presentation Purified
Reactivity Hu
Applications F, IP, WB
Clonality Monoclonal
Clone GL183
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P43628
Immunogen:
NK cell clone E57. Spleen cells from immunised BALB/c mice were fused with cells of the mouse P3UI myeloma cell line.
GeneID:
3804
Isotype control AM03095PU-N, SM10P (for use in human samples)
Application Flow cytometry: 1/10 - 1/50; Use 10µl of the suggested working dilution to label 10e6 cells in 100µl.
Immunoprecipitation.
Background CD158b is a 58kD glycoprotein expressed by a subset of NK cells (also known as p58.2 antigen). The antigen is a members of the newly described natural killer cell receptor family. CD158b functions as a receptor specific for HLA Class I molecules, including Cw3 and related HLA-C alleles. This antibody can restore the lysis by human NK clones of otherwise lysis protected targets expressing Cw3.
Concentration 1.0 mg/ml
General Readings
  1. Moretta A, Tambussi G, Bottino C, Tripodi G, Merli A, Ciccone E, et al. A novel surface antigen expressed by a subset of human CD3- CD16+ natural killer cells. Role in cell activation and regulation of cytolytic function. J Exp Med. 1990 Mar 1;171(3):695-714. PubMed PMID: 2137855. (Free PMC Article available)
  2. Moretta A, Vitale M, Bottino C, Orengo AM, Morelli L, Augugliaro R, et al. P58 molecules as putative receptors for major histocompatibility complex (MHC) class I molecules in human natural killer (NK) cells. Anti-p58 antibodies reconstitute lysis of MHC class I-protected cells in NK clones displaying different specificities. J Exp Med. 1993 Aug 1;178(2):597-604. PubMed PMID: 8340759. (Free PMC Article available)
  3. Ciccone E, Pende D, Viale O, Di Donato C, Tripodi G, Orengo AM, et al. Evidence of a natural killer (NK) cell repertoire for (allo) antigen recognition: definition of five distinct NK-determined allospecificities in humans. J Exp Med. 1992 Mar 1;175(3):709-18. PubMed PMID: 1371301. (Free PMC Article available)
  4. Moretta A, Biassoni R, Bottino C, Pende D, Vitale M, Poggi A, et al. Major histocompatibility complex class I-specific receptors on human natural killer and T lymphocytes. Immunol Rev. 1997 Feb;155:105-17. PubMed PMID: 9059886.
  5. Moretta A, Sivori S, Vitale M, Pende D, Morelli L, Augugliaro R, et al. Existence of both inhibitory (p58) and activatory (p50) receptors for HLA-C molecules in human natural killer cells. J Exp Med. 1995 Sep 1;182(3):875-84. PubMed PMID: 7650491. (Free PMC Article available)
  6. Biassoni R, Cantoni C, Falco M, Verdiani S, Bottino C, Vitale M, et al. The human leukocyte antigen (HLA)-C-specific "activatory" or "inhibitory" natural killer cell receptors display highly homologous extracellular domains but differ in their transmembrane and intracytoplasmic portions. J Exp Med. 1996 Feb 1;183(2):645-50. PubMed PMID: 8627176. (Free PMC Article available)
  7. Long EO, Colonna M, Lanier LL. Inhibitory MHC class I receptors on NK and T cells: a standard nomenclature. Immunol Today. 1996 Feb;17(2):100. PubMed PMID: 8808061.
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Buffer System:
PBS containing 0.09% Sodium Azide and 0.1% Bovine Serum Albumin
State:
Liquid purified IgG
Purified
Specificity
Specificity:
This antibody recognises the CD158b cell surface antigen. This antibody also recognises a 50kD molecule, which is highly homologous to p58.2 in the extracellular domain, but has a shorter cytoplasmic tail.
Species:
Human.
Gene ID 3804
FocusOn and Reviews
FocusOns:

Accessory Products

Proteins and/or Positive Controls

Proteins for CD158b / KIR2DL3 (6 products)

Catalog No. Species Pres. Purity   Source  

CD158b / KIR2DL3

CD158b / KIR2DL3 Human Purified > 95 % by SDS-PAGE E. coli
0.1 mg / €400.00
  OriGene Technologies GmbH

CD158b / KIR2DL3

CD158b / KIR2DL3 Human Purified > 95 % by SDS-PAGE E. coli
0.5 mg / €1,070.00
  OriGene Technologies GmbH

CD158b / KIR2DL3

CD158b / KIR2DL3 Human
  Abnova Taiwan Corp.

CD158b / KIR2DL3

CD158b / KIR2DL3 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

CD158b / KIR2DL3

CD158b / KIR2DL3 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

CD158b / KIR2DL3

SDS-Page: KIR2DL3 Protein [NBC1-18410] - KIR2DL3, 22.2kDa (202aa), confirmed by MALDI-TOF with a purity of 95% by SDS - PAGE Protein
  Novus Biologicals Inc.

Positive controls for CD158b / KIR2DL3 (1 products)

Catalog No. Species Pres. Purity   Source  

CD158b / KIR2DL3 Lysate(Denatured)

CD158b / KIR2DL3 Lysate(Denatured)
  Abnova Taiwan Corp.
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