AM10038FC-N CD175s antibody

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0.2 mg / €390.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

Quick Overview

Mouse anti Human CD175s STN219


Product Description for CD175s

Mouse anti Human CD175s STN219.
Presentation: FITC
Product is tested for Frozen Sections, Immunocytochemistry/Immunofluorescence, Flow Cytometry.

Properties for CD175s

Product Category Primary Antibodies
Quantity 0.2 mg
Synonyms NeuAc a GalNAc, Sialosyl Tn, Sialosyl-Tn, Sialyl Tn, sTn
Presentation FITC
Reactivity Hu
Applications C, F, ICC/IF
Clonality Monoclonal
Clone STN219
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Ovine submaxillary mucin (OSM).
Molecular weight of antigen: Several HM weight glycoproteins.
Epitope: NeuAc alphaGalNacOSer/Thr.
Isotype control SM10F (for use in human samples)
Application Suitable for Immunohistochemistry and Immunocytochemistry (Frozen or Formalin-Fixed Paraffin-Embedded (FFPE) tissue sections and cell smears). 
For IHC dilute concentared antibody at 1/50-1/100, use streptavidin~biotin system or polymer system, incubate 30 minutes at room temperature.
Immunofluorescence: 10-20 µg/ml (1/10-1/20), incubate for 2 hours in the dark at RT or it can also be incubated overnight at 4ºC.
Flow Cytometry: 0.2-1.0 µg/0.1 ml (1/200-1/1,000).
Recommended Positive Control: Human gastrointestinal tumor, prostate, ovary ca.
Background Sialosyl Tn antigen also known as CD175s is expressed in the majority of colon, gastric, breast and lung carcinoma.
The sialosyl Tn has limited expression to being absent in normal colon, pancreas, stomach, ovary, and prostate. This antigen is detected in several adenocarcinomas. It appears to be useful marker for poorly differentiated colon adenocarcinomas. This antibody stains colon carcinomas and ovarian mucinous carcinomas producing apical cytoplasmic, apical membrane or luminal staining patterns. It is negative with majority of adult tissue sections.
Concentration 0.2 mg/ml
General Readings
  1. Gold, DV et. al. Tumor Biol. 9: 137-144, 1990.
  2. Siddiki, B et. al. Intl. J Cancer, 54: 467-474, 1993.
  3. Itzkowitz,S, S et. al. Cancer, 66: 1960-1966, 1990.
  4. Wang, F et. al. J Histochem. Cytochem. 49:1581-1591, 2001.
  5. Ju, T et. al. Cancer Res. 68: 1636-1646, 2008.
  6. Pinho, S, et. al. Cancer Lett. 249: 157, 2006.
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
This product is photosensitive and should be protected from light.
Shelf life: One year from despatch.
Buffer System:
PBS, pH 7.4
0.05% Sodium Azide
1% BSA
Liquid purified Ig fraction.
The specificity of this antibody is similar to B 72.3 antibody.
Cellular Localization: Cytoplasmic, apical membrane, luminal.

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