SM1867 CD176 (Thomsen-Friedenreich antigen) antibody

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Mouse anti Human CD176 (Thomsen-Friedenreich antigen) A78-G/A7


Product Description for CD176 (Thomsen-Friedenreich antigen)

Mouse anti Human CD176 (Thomsen-Friedenreich antigen) A78-G/A7.
Presentation: Supernatant
Product is tested for Frozen Sections, Enzyme Immunoassay, Flow Cytometry, Paraffin Sections.

Properties for CD176 (Thomsen-Friedenreich antigen)

Product Category Primary Antibodies
Quantity 1 ml
Presentation Supernatant
Reactivity Hu
Applications C, E, F, P
Clonality Monoclonal
Clone A78-G/A7
Host Mouse
Isotype IgM
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Application Flow cytometry: 1/5 - 1/10; use 10 µl of the suggested working dilution to label 106 cells. Immunohistochemistry on frozen and paraffin embedded sections: 1/5 - 1/10. This product does not require protein digestion pre-treatment of paraffin sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections. Positive control tissue: Breast Carcinoma. ELISA. This antibody is reported to work in Western blotting applications. Other applications not tested. Optimal dilutions of this antibody are dependent on conditions and should be determined by the user.
General Readings
  1. Karsten U, Butschak G, Cao Y, Goletz S, Hanisch FG. A new monoclonal antibody (A78-G/A7) to the Thomsen-Friedenreich pan-tumor antigen. Hybridoma. 1995 Feb;14(1):37-44. PubMed PMID: 7539400.
Storage Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.
DO NOT FREEZE. This product should be stored undiluted. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
This antibody recognises the human CD176 cell surface antigen, also known as the Thomsen-Friedenreich (TF) antigen.
CD176 is a carbohydrate epitope carried by a variety of carrier proteins, such as MUC1. The epitope is expressed by varying levels by carcinomas (e.g. upon 85% of breast carcinoma), but it is masked or absent on almost all normal adult tissues. CD176 is also expressed by some CD34+ stem cells and by the KG1 cell line.

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