AM20860LE-N CD178 / Fas Ligand antibody

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0.5 mg / €560.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

Quick Overview

Hamster anti Mouse, Rat CD178 / Fas Ligand MFL4

AM20860LE-N

Product Description for CD178 / Fas Ligand

Hamster anti Mouse, Rat CD178 / Fas Ligand MFL4.
Presentation: Low Endotoxin
Product is tested for Functional assay, Flow Cytometry.

Properties for CD178 / Fas Ligand

Product Category Primary Antibodies
Target Category
Quantity 0.5 mg
Synonyms APT1LG1, APTL, Apoptosis antigen ligand, CD95L protein, FASL, FASLG, Fas antigen ligand, TNFSF6, Tumor necrosis factor ligand superfamily member 6
Presentation Low Endotoxin
Reactivity Ms, Rt
Applications F, FN
Clonality Monoclonal
Clone MFL4
Host Hamster
Isotype IgG
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P41047
Immunogen:
B6 Mouse FasL/BHK cells
GeneID:
14103
Add. information Endotoxin Level: < 0.01 EU/µg
Application Flow Cytometry: Use 10 µl of 1/25-1/200 diluted antibody to label 1x10e6 cells in 100 µl.
Functional Assays.
Background CD178, otherwise known as Fas Ligand/ CD95L, a 40kDa type II transmembrane glycoprotein and member of the TNF/NGF superfamily, expressed by activated T cells and NK cells, which can be induced on a variety of cells by radiation, heat shock, chemotherapeutic agents and viral infection.
CD178 acts as a key effector of cytotoxicity and in the regulation of immune responses. The binding of CD178 to its receptor CD95 (Fas), induces Fas-mediated apoptosis of target cells, and may be involved in the induction of peripheral tolerance and neutrophil chemotaxis. The binding of decoy receptor 3 (DcR3) to CD178 has been shown to inhibit CD178-mediated apoptosis.
Concentration 1.0 mg/ml
General Readings
  1. Trinite, B. et al. (2000) A subset of cytolytic dendritic cells in rat. J. Immunol. 165: 4202-4208.
  2. Watanabe T, Yoshida M, Shirai Y, Yamori M, Yagita H, Itoh T, et al. Administration of an antigen at a high dose generates regulatory CD4+ T cells expressing CD95 ligand and secreting IL-4 in the liver. J Immunol. 2002 Mar 1;168(5):2188-99. PubMed PMID: 11859105.
  3. Kayagaki N, Yamaguchi N, Nagao F, Matsuo S, Maeda H, Okumura K, et al. Polymorphism of murine Fas ligand that affects the biological activity. Proc Natl Acad Sci U S A. 1997 Apr 15;94(8):3914-9. PubMed PMID: 9108079. (Free PMC Article available, 5 images available)
Storage Store the antibody undiluted at -20°C.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Affinity Chromatography on Protein G
Buffer System:
PBS without preservatives
State:
Liquid purified IgG fraction
Low Endotoxin
Specificity
Specificity:
Recognizes both Mouse and Rat CD178.
Clone MFL4 is reported to block CD178/CD95 induced apoptosis.
Species:
Mouse and Rat.
Gene ID 14103

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