SM2268PS CD178 / Fas Ligand antibody

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0.1 mg / €280.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

Quick Overview

Mouse anti Human CD178 / Fas Ligand 1OF2

Product Description for CD178 / Fas Ligand

Mouse anti Human CD178 / Fas Ligand 1OF2.
Presentation: Purified
Product is tested for Enzyme Immunoassay, Immunoprecipitation, Flow Cytometry.

Properties for CD178 / Fas Ligand

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms APT1LG1, APTL, Apoptosis antigen ligand, CD95L protein, FASL, FASLG, Fas antigen ligand, TNFSF6, Tumor necrosis factor ligand superfamily member 6
Presentation Purified
Reactivity Hu
Applications E, F, IP
Clonality Monoclonal
Clone 1OF2
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P48023
Immunogen:
Spleen cells from immunised BALB/c mice were fused with cells of the P3U1 myeloma cell line.
GeneID:
356
Isotype control AM03095PU-N, SM10P (for use in human samples)
Application Flow Cytometry: Use 10 µl of 1/25-1/50 diluted antibody to label 1x10e6 cells in 100 µl.
ELISA.
Immunoprecipitation.
Background CD178 is a 40kDa glycoprotein also known as Fas ligand (CD95L). CD178 is a member of the TNF family, which is expressed by activated T lymphocytes and NK cells. The protein may exist as either a membrane bound or a cleaved soluble form. CD178 plays an important role in T cell development and cytotoxicity. Binding of CD178 to Fas (CD95) results in the induction of apoptosis.
Concentration 1.0 mg/ml
General Readings
  1. Legembre P, Moreau P, Daburon S, Moreau JF, Taupin JL. Potentiation of Fas-mediated apoptosis by an engineered glycosylphosphatidylinositol-linked Fas. Cell Death Differ. 2002 Mar;9(3):329-39. PubMed PMID: 11859415.
  2. Ferry-Dumazet H, Garnier O, Mamani-Matsuda M, Vercauteren J, Belloc F, Billiard C, et al. Resveratrol inhibits the growth and induces the apoptosis of both normal and leukemic hematopoietic cells. Carcinogenesis. 2002 Aug;23(8):1327-33. PubMed PMID: 12151351.
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Affinity Chromatography on Protein G
Buffer System:
PBS, pH7.4 containing 0.09% Sodium Azide as preservative.
State:
Liquid purified IgG fraction.
Purified
Specificity
Specificity:
This antibody recognises the CD178 cell surface antigen. This antibody may be used in combination with clone 14C2 (MCA2409) in ELISA assays.
Clone 10F2 is reported to block the activity of Fas ligand. We recommend the use of SM2268LE for such studies.
Species:
Human.
Gene ID 356

Accessory Products

Proteins and/or Positive Controls

Proteins for CD178 / Fas Ligand (18 products)

Catalog No. Species Pres. Purity   Source  

CD178 / Fas Ligand

CD178 / Fas Ligand Human > 95 %
Preparation: .
Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.
CHO cells
10 µg / €239.00
  OriGene Technologies, Inc.

CD178 / Fas Ligand

CD178 / Fas Ligand Human > 90 %
Preparation: .
Purity Detail: >90%, as determined by Coomassie stained SDS-PAGE.
CHO cells
10 µg / €147.00
  OriGene Technologies, Inc.

CD178 / Fas Ligand (N-term, His-tagged)

CD178 / Fas Ligand Human > 95 % pure by SDS-PAGE gel and HPLC analyses CHO cells
  OriGene Technologies GmbH

CD178 / Fas Ligand (N-term, His-tagged)

CD178 / Fas Ligand Human > 95 % pure by SDS-PAGE gel and HPLC analyses CHO cells
  OriGene Technologies GmbH

CD178 / Fas Ligand (His-tag)

CD178 / Fas Ligand Human Purified > 95 % by SDS-PAGE & HPLC analysis CHO cells
10 µg / €330.00
  OriGene Technologies GmbH

CD178 / Fas Ligand (130-281, His-tag)

CD178 / Fas Ligand Human Purified > 90 % by SDS - PAGE E. coli
0.5 mg / €730.00
  OriGene Technologies GmbH

CD178 / Fas Ligand (130-281, His-tag)

CD178 / Fas Ligand Human Purified > 90 % by SDS - PAGE E. coli
0.1 mg / €300.00
  OriGene Technologies GmbH

CD178 / Fas Ligand (Extracell. Dom.)

CD178 / Fas Ligand Human > 98 % CHO cells
  OriGene Technologies GmbH

CD178 / Fas Ligand (Extracell. Dom.)

CD178 / Fas Ligand Human > 98 % CHO cells
  OriGene Technologies GmbH

CD178 / Fas Ligand

CD178 / Fas Ligand Human
  Abnova Taiwan Corp.

CD178 / Fas Ligand

CD178 / Fas Ligand Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

CD178 / Fas Ligand

CD178 / Fas Ligand Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

CD178 / Fas Ligand

CD178 / Fas Ligand Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

CD178 / Fas Ligand

CD178 / Fas Ligand Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

CD178 / Fas Ligand

CD178 / Fas Ligand Human
  Abnova Taiwan Corp.

CD178 / Fas Ligand

CD178 / Fas Ligand Human Purified > 98 % CHO cells
  Alpha Diagnostic Intl. Inc.

CD178 / Fas Ligand

CD178 / Fas Ligand Human Purified > 98 % CHO cells
  Alpha Diagnostic Intl. Inc.

CD178 / Fas Ligand (103-281)

CD178 / Fas Ligand Human HEK293 cells
  Kamiya Biomedical Company

Positive controls for CD178 / Fas Ligand (2 products)

Catalog No. Species Pres. Purity   Source  

CD178 / FAS Ligand Lysate(Denatured)

CD178 / FAS Ligand Lysate(Denatured)
  Abnova Taiwan Corp.

Fas Ligand Lysate

Western Blot: Fas Ligand Lysate [NBL1-10600] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for FASLG
  Novus Biologicals Inc.
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