BM4017 CD205 / DEC-205 antibody

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0.1 mg / €290.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

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Rat anti Mouse CD205 / DEC-205 NLDC-145

BM4017

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Product Description for CD205 / DEC-205

Rat anti Mouse CD205 / DEC-205 NLDC-145.
Presentation: Purified
Product is tested for Frozen Sections, Flow Cytometry.

Properties for CD205 / DEC-205

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms C-type lectin domain family 13 member B, CLEC13B, DEC205, LY75, Lymphocyte antigen 75, gp200-MR6
Presentation Purified
Reactivity Ms
Applications C, F
Clonality Monoclonal
Clone NLDC-145
Host Rat
Isotype IgG2a
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
Q60767
Immunogen:
Mouse lymph node tissue.
GeneID:
17076
Isotype control SM15P, SM15PX
Application Immunohistochemistry on Frozen Sections: 0.5 μg/ml (1/400).
Recommended Positive Control: Mouse spleen.
Does not react on routinely processed paraffin sections.
Has been described to work in FACS.
Background Mouse CD205, also known as DEC-205 (dendritic and epithelial cells, 205 kDa). CD205 is an integral membrane glycoprotein involved in antigen uptake, trafficking and presentation that improves the induction of antigen-specific T cell immunity. CD205 is highly expressed by CD8+ dendritic cells (DCs) and also expressed at different levels by bone marrow Gr1+ cells, Langerhans cells, (BMDC) bone marrow derived DCs and thymic epithelial cells.
DEC-205 is apparently a receptor involved in antigen-processing by dendritic cells.
Concentration 0.2 mg/ml (after reconstitution)
Protocols Protocol with frozen, ice-cold acetone-fixed sections:
The whole procedure is performed at room temperature
1. Wash in PBS
2. Block endogenous peroxidase
3. Wash in PBS
4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber
5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber
6. Wash in PBS
7. Incubate with secondary antibody (peroxidase-conjugated goat anti rat IgG (H+L) minimal-cross reaction to mouse) for 1h in a humid chamber
8. Wash in PBS
9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min.
10. Wash in PBS
11. Counterstain with Mayer's hemalum.
General Readings
  1. Kraal G, Breel M, Janse M, Bruin G. Langerhans' cells, veiled cells, and interdigitating cells in the mouse recognized by a monoclonal antibody. J Exp Med. 1986 Apr 1;163(4):981-97. PubMed PMID: 3950549. (Free PMC Article available)
  2. Breel M, Mebius RE, Kraal G. Dendritic cells of the mouse recognized by two monoclonal antibodies. Eur J Immunol. 1987 Nov;17(11):1555-9. PubMed PMID: 3678361.
  3. Swiggard WJ, Mirza A, Nussenzweig MC, Steinman RM. DEC-205, a 205-kDa protein abundant on mouse dendritic cells and thymic epithelium that is detected by the monoclonal antibody NLDC-145: purification, characterization, and N-terminal amino acid sequence. Cell Immunol. 1995 Oct 15;165(2):302-11. PubMed PMID: 7553896.
  4. Martinez del Hoyo, G., P. Martin, H. Hernandez Vargas, S. Ruiz, C. Fernandez Arias, C. Ardavin: Characterization of a common precursor population for dendtiric cells. Nature 415: 1043-47 (2002).
Storage Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month 
or (in aliquots) at -20°C long term.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Affinity Chromatography
Buffer System:
PBS, pH 7.2
Preservatives:
0.05% (v/v) Kathon CG
Stabilizers:
5 mg/ml BSA
State:
Lyophilized purified IgG fraction
Purified
Reconstitution:
Restore by adding 0.5 ml distilled water.
Specificity
Specificity:
Reacts with nonlymphoid dendritic cells: interdigitating cells (IDC), veiled cells and Langerhans cells, thymic epithelial cells.
Monoclonal antibody NLDC-145 identifies Ia positive interdigitating cells, veiled cells and Langerhans cells of the skin and their in vitro counterparts.
The antigen is expressed at high levels by dendritic cells and thymic epithelial cells.
The antigen detected by NLDC-145 is an integral membrane glycoprotein with an apparent mass of 205kDa, also known as DEC-205.
Species:
Mouse.
Gene ID 17076
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