NB120-6396 CD235a / CD235b antibody

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Mouse anti Human CD235a / CD235b E3


Product Description for CD235a / CD235b

Mouse anti Human CD235a / CD235b E3.
Presentation: Purified
Product is tested for Enzyme Immunoassay, Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot, Flow Cytometry.

Properties for CD235a / CD235b

Product Category Primary Antibodies
Quantity 0.2 ml
Synonyms CD235A, CD235b, GPA, GPB, GYPA, GYPB, Glycophorin-A, Glycophorin-B, MN sialoglycoprotein, PAS-2, PAS-3, SS-active sialoglycoprotein, Sialoglycoprotein alpha, Sialoglycoprotein delta
Presentation Purified
Reactivity Hu
Applications E, F, ICC/IF, WB
Clonality Monoclonal
Clone E3
Host Mouse
Isotype IgG1
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

human thymus
Isotype control AM03095PU-N, SM10P (for use in human samples)
Application ELISA, flow cytometry / FACS analysis, immunofluorescence, Western Blot, ELISA: Use at a dilution of 1/6400. FACS: Use at a dilution of 1/400 (10ul per million cells). WB: Use at a dilution of 1/400. Agglutination: Use at a dilution of 1/1000Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user., flow cytometry / FACS analysis , ELISA , immunofluorescence , Western Blot
Background Glycophorins A (GYPA) and B (GYPB) are major sialoglycoproteins of the human erythrocyte membrane which bear the antigenic determinants for the MN and Ss blood groups. GYPA gene consists of 7 exons and has 97% sequence homology with GYPB from the 5' UTR to the coding sequence encoding the first 45 amino acids. GYPB accounts for S, s and U specificities. GPA and GPB provide the cells with a large mucin-like surface and it has been suggested this provides a barrier to cell fusion, so minimizing aggregation between red blood cells in the circulation. In addition to the M or N and S or s antigens, that commonly occur in all populations, about 40 related variant phenotypes have been identified. These variants include all the variants of the Miltenberger complex and several isoforms of Sta; also, Dantu, Sat, He, Mg, and deletion variants Ena, S-s-U- and Mk. Most of the variants are resulted from gene recombinations between GYPA and GYPB. These antigens are expressed on early erythroblasts, late erythroblasts, erythroblasts, mature erythrocytes and the cells of erythroid cell lines K562 and HEL, but not on all other cells (mature erythrocytes are characteristically CD235a positive and CD45 and CD71 negative).
General Readings General / background references:Telen MJ et al. Human erythrocyte antigens. III. Characterization of a panel of murine monoclonal antibodies that react with human erythrocyte and erythroid precursor membranes. Vox Sang 52:236-43 (1987). PubMed PMID: 3474823
Telen MJ & Bolk TA Human red cell antigens. IV. The abnormal sialoglycoprotein of Gerbich-negative red cells. Transfusion 27:309-14 (1987). PubMed PMID: 3603658
Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
IgG purified
Buffer System:
0.02% Sodium Azide
IgG purified
Binds to red blood cell glycophorins A & B (glycophorin

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