SM605P CD25 / IL2RA antibody

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Mouse anti Rabbit CD25 / IL2RA KEI-ALPHA1


Product Description for CD25 / IL2RA

Mouse anti Rabbit CD25 / IL2RA KEI-ALPHA1.
Presentation: Purified
Product is tested for Frozen Sections, Immunoprecipitation, Flow Cytometry.

Properties for CD25 / IL2RA

Product Category Primary Antibodies
Quantity 0.25 mg
Synonyms IL-2 receptor alpha subunit, IL-2-RA, IL2-RA, IL2RA, Interleukin-2 receptor alpha chain, TAC antigen, p55
Presentation Purified
Reactivity Rb
Applications C, F, IP
Clonality Monoclonal
Host Mouse
Isotype IgG2b
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Cell membrane preparation of HTLV-1 transformed rabbit T cell line.
Spleen cells of immunised BALB/c mice were fused with cells of the mouse PAI myeloma cell line.
Isotype control AM03110PU-N, SM12P
Application Flow Cytometry: Use 10µl of 1/100-1/200 diluted antibody to label 10e6 cells in 100 µl.
Immunohistochemistry on Frozen Sections.
Background The Interleukin 2 Receptor alpha and beta chains, together with the common gamma chain, constitute the high affinity IL2 receptor present on activated T and B cells, thymocyte subset, pre B cells and T regulatory cells. Homodimeric alpha chains result in low affinity receptor, while homodimeric beta chains produce a medium affinity receptor. Normally an integral membrane protein, soluble IL2 Receptor alpha has been isolated and determined to result from extracellular proteolysis. Alternately spliced IL2 Receptor alpha mRNAs have been isolated, but the significance of each is presently unknown.
Concentration 1.0 mg/ml
General Readings
  1. Kotani, M. et al. (1993) Generation of monoclonal antibodies to the rabbit IL-2 receptor a chain (CD25) and its distribution in HTLV-1 transformed rabbit T cells. J. Cancer. Res. 84: 770-775.
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Affinity Chromatography on Protein G.
Buffer System:
PBS, pH 7.4 containing 0.09% Sodium Azide as preservative.
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Liquid purified IgG fraction.
This antibody recognises rabbit CD5, a 55kD cell surface antigen known as the interleukin-2 (IL-2) receptor alpha chain. In rabbits, CD25 is expressed on activated T lymphocytes.
The kei-al antibody is reported to inhibit the binding of IL-2 to its receptor. (1)
Rabbit. Does not react with Human.

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