AM26519RP-N CD268 / BAFFR antibody

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50 Tests / €370.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

Quick Overview

Mouse anti CD268 / BAFFR 8A7

Product Description for CD268 / BAFFR

Mouse anti CD268 / BAFFR 8A7.
Presentation: PE

Properties for CD268 / BAFFR

Product Category Primary Antibodies
Target Category
Quantity 50 Tests
Synonyms B cell-activating factor receptor, BAFF receptor, BAFF-R, BLyS receptor 3, BR3, TNFRSF13C, Tumor necrosis factor receptor superfamily member 13C
Presentation PE
Clonality Monoclonal
Clone 8A7
Host Mouse
Isotype IgG2a
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
Q96RJ3
Immunogen:
Human CD268/BAFF-R/BR3 transfectant
GeneID:
115650
Add. information

This product was originally produced by MBL International.

Application Flow cytometry: 20 μl (ready for use).
For details see protocol below.
Background BAFF (B cell-activating factor belonging to the TNF family) is a membrane protein expressed by dendritic cells, monocytes, macrophages, follicular dendritic cells, activated T cells, activated neutrophils, and malignant B cells. BAFF, also known as BLyS (B lymphocyte stimulator), is a potent B cell growth factor. Proteolytic cleavage can result in the release of a soluble trimeric BAFF which binds to the BAFF-R/BR3, BCMA and TACI. BAFF-R/BR3 is the principal receptor for B cell survival and responses induced by BAFF.
Protocols

Flow cytometric analysis for floating cells
We usually use Fisher tubes or equivalents as reaction tubes for all step described below.
1) Wash the cells 3 times with washing buffer [PBS containing 2% fetal calf serum (FCS) and 0.1% NaN3].
2) Resuspend the cells with washing buffer (5x10e6 cells/mL).
3) Add 50 µL of the cell suspension into each tube, and centrifuge at 500 x g for 1 minute at room temperature (20~25 o C). Remove supernatant by careful aspiration.
4) Add 20 µL of Clear Back (human Fc receptor blocking reagent) to the cell pellet after tapping. Mix well and incubate for 5 minutes at room temperature.
5) Add 20 µL of the PE labeled CD268/BAFF-R/BR3 (8A7). Mix well and incubate for 30 minutes at room temperature.
6) Add 1 mL of the washing buffer followed by centrifugation at 500 x g for 1 minute at room temperature. Remove supernatant by careful aspiration.
7) Resuspend the cells with 500 µL of the washing buffer and analyze by a flow cytometer.
(Positive control for Flow cytometry; Raji)

General Readings
  1. Nakamura, N., et al ., Virchows Arch 447 , 53-60 (2005).
  2. Mackay, F., et al ., Annu. Rev. Immunol. 21 , 231-264 (2004).
Storage Store at 2-8 °C.
Shelf life: one year from despatch.
Format
Label:
PE
Purification:
Protein A agarose
Buffer System:
50 tests in 1 mL volume of PBS containing 1% BSA and 0.09% NaN3
State:
Liquid Ig fraction
Specificity
Specificity:
This antibody reacts with human CD268/BAFF-R/BR3.
Gene ID 115650

Accessory Products

Proteins and/or Positive Controls

Proteins for CD268 / BAFFR (9 products)

Catalog No. Species Pres. Purity   Source  

CD268 / BAFFR

CD268 / BAFFR Human > 95 %
Preparation: .
Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.
E. coli

Regular Price: 50 µg / €199.00

Special Price: 50 µg / €139.00

  OriGene Technologies, Inc.

CD268 / BAFFR

CD268 / BAFFR Human Purified > 95 % pure by SDS-PAGE gel and HPLC analyses E. coli
  Acris Antibodies GmbH

CD268 / BAFFR

CD268 / BAFFR Human Purified > 95 % pure by SDS-PAGE gel and HPLC analyses E. coli
  Acris Antibodies GmbH

CD268 / BAFFR

CD268 / BAFFR Human
  Abnova Taiwan Corp.

CD268 / BAFFR

CD268 / BAFFR Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

CD268 / BAFFR

CD268 / BAFFR Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

CD268 / BAFFR

CD268 / BAFFR Human
  Abnova Taiwan Corp.

CD268 / BAFFR

CD268 / BAFFR Human Purified
  Novus Biologicals Inc.

CD268 / BAFFR

CD268 / BAFFR Human Purified
  Novus Biologicals Inc.

Positive controls for CD268 / BAFFR (2 products)

Catalog No. Species Pres. Purity   Source  

BAFF Receptor Lysate

Western Blot: BAFF Receptor Lysate [NBL1-17149] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for TNFRSF13C
  Novus Biologicals Inc.

TNFRSF13C 293T Cell Transient Overexpression Lysate(Denatured)

TNFRSF13C 293T Cell Transient Overexpression Lysate(Denatured)
  Abnova Taiwan Corp.
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