AM01313PU-N CD273 / PDL2 antibody

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0.25 mg / €460.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

Quick Overview

Rat anti Mouse CD273 / PDL2 TY25

AM01313PU-N

Product Description for CD273 / PDL2

Rat anti Mouse CD273 / PDL2 TY25.
Presentation: Purified
Product is tested for Frozen Sections, Immunoprecipitation, Flow Cytometry.

Properties for CD273 / PDL2

Product Category Primary Antibodies
Target Category
Quantity 0.25 mg
Synonyms B7DC, PD-1-ligand 2, PD-L2, PDCD1 ligand 2, PDCD1L2, PDCD1LG2, Programmed cell death 1 ligand 2, Programmed death ligand 2
Presentation Purified
Reactivity Ms
Applications C, F, IP
Clonality Monoclonal
Clone TY25
Host Rat
Isotype IgG2a
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
Q9WUL5
Immunogen:
B7-DC transfected RAW264.7 cells. Lymph node cells from immunised SD rats were fused with the cells of the P3U1 myeloma cell line.
GeneID:
58205
Isotype control SM15P, SM15PX
Application Immunoprecipitation.
Immunohistochemistry on frozen sections.
Flow Cytometry: 1/100 - 1/200; Use 10µl of the suggested working dilution to label 10e6 cells in 100µl.
Background The expression of CD273 is restricted to a subpopulation of dendritic cells (DCs), and DCs and macrophages that have been stimulated with interferon gamma, GM-CSF or IL-4. CD273 has been identified as a ligand for PD-1(CD279), along with another B7 family member B7-H1 (CD279).
Studies suggest that both CD273 and B7-H1 co-stimulate or inhibit T cell proliferation and cytokine production. Antibodies produced by clone TY25 are reported to block the binding of CD273 (B7-DC) to it's receptor.
Concentration 1.0 mg/ml
General Readings
  1. Matsumoto K, Inoue H, Nakano T, Tsuda M, Yoshiura Y, Fukuyama S, et al. B7-DC regulates asthmatic response by an IFN-gamma-dependent mechanism. J Immunol. 2004 Feb 15;172(4):2530-41. PubMed PMID: 14764726.
  2. Yamazaki T, Akiba H, Iwai H, Matsuda H, Aoki M, Tanno Y, et al. Expression of programmed death 1 ligands by murine T cells and APC. J Immunol. 2002 Nov 15;169(10):5538-45. PubMed PMID: 12421930.
  3. Kanai T, Totsuka T, Uraushihara K, Makita S, Nakamura T, Koganei K, et al. Blockade of B7-H1 suppresses the development of chronic intestinal inflammation. J Immunol. 2003 Oct 15;171(8):4156-63. PubMed PMID: 14530338.
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Affinity chromatography on Protein G
Buffer System:
PBS, pH 7.4 containing 0.09% Sodium Azide
State:
Liquid purified Ig
Purified
Specificity
Specificity:
This antiobdy recognises mouse CD273, a 42kD member of the B7 family, otherwise known as B7-dendritic cell (B7-DC), or programmed death ligand-2 (PD-L2).
Species:
Mouse.
Gene ID 58205
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