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AP30656PU-N CD273 / PDL2 antibody

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Rabbit anti Human, Mouse, Rat CD273 / PDL2

AP30656PU-N

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  • AP30656PU-N
  • AP30656PU-N

Product Description for CD273 / PDL2

Rabbit anti Human, Mouse, Rat CD273 / PDL2.
Presentation: Aff - Purified
Product is tested for Immunocytochemistry/Immunofluorescence, Enzyme Immunoassay, Paraffin Sections, Western blot / Immunoblot.

Properties for CD273 / PDL2

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms B7DC, PD-1-ligand 2, PD-L2, PDCD1 ligand 2, PDCD1L2, PDCD1LG2, Programmed cell death 1 ligand 2, Programmed death ligand 2
Presentation Aff - Purified
Reactivity Hu, Ms, Rt
Applications E, ICC/IF, P, WB
Clonality Polyclonal
Host Rabbit
Isotype IgG
Molecular weight 30 kDa (Predicted), 32 kDa (Observed).
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
Q9BQ51
Immunogen:
PDL-2 antibody was raised against a 16 amino acid peptide from near the center of human PDL-2.
GeneID:
80380
Add. information Blocking peptide available: AP30656CP-N
Application ELISA.
Western blot: 0.5-2 μg/ml.
Immunohistochemistry on Paraffin Sections: 2.5 μg/ml.
Immunofluorescence: 20 µg/ml.
Background Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by co gnate peptides bound to MHC molecules on antigen-presenting cells (APC). T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PDL-1 and PDL-2, both of which are thought act as a negative regulator of T cell activation. However, it has been suggested that PDL-2 can act to stimulate an immunogenic response through and alternative receptor from PD-1. At least three isoforms of PDL-2 are known to exist; this antibody is specific to the largest isoform.
Concentration 1.0 mg/ml
General Readings
  1. Holling TM, Schooten E, van Den Elsen PJ. Function and regulation of MHC class II molecules in T-lymphocytes: of mice and men. Hum Immunol. 2004 Apr;65(4):282-90. PubMed PMID: 15120183.
  2. Ishida Y, Agata Y, Shibahara K, Honjo T. Induced expression of PD-1, a novel member of the immunoglobulin gene superfamily, upon programmed cell death. EMBO J. 1992 Nov;11(11):3887-95. PubMed PMID: 1396582. (Free PMC Article available)
  3. LaGier AJ, Pober JS. Immune accessory functions of human endothelial cells are modulated by overexpression of B7-H1 (PDL1). Hum Immunol. 2006 Aug;67(8):568-78. Epub 2006 May 19. PubMed PMID: 16916652.
  4. Zhang Y, Chung Y, Bishop C, Daugherty B, Chute H, Holst P, et al. Regulation of T cell activation and tolerance by PDL2. Proc Natl Acad Sci U S A. 2006 Aug 1;103(31):11695-700. Epub 2006 Jul 24. PubMed PMID: 16864790. (Free PMC Article available, 6 images available)
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Peptide Affinity Chromatography
Buffer System:
PBS
Preservatives:
0.02% Sodium Azide
State:
Liquid purified Ig fraction
Aff - Purified
Species Reactivity
Species reactivity (tested):
Human, Mouse, Rat.
Specificity
Specificity:
This antibody detects CD273 / PDL2.
Gene ID 80380
FocusOn and Reviews
FocusOns:

Accessory Products

Proteins and/or Positive Controls

Proteins for CD273 / PDL2 (11 products)

Catalog No. Species Pres. Purity   Source  

CD273 / PDL2

CD273 / PDL2 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €748.00
  OriGene Technologies, Inc.

CD273 / PDL2

CD273 / PDL2 Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €748.00
  OriGene Technologies, Inc.

CD273 / PDL2

CD273 / PDL2 Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €748.00
  OriGene Technologies, Inc.

CD273 / PDL2

CD273 / PDL2 Human > 95 %
Preparation: .
Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
10 µg / €299.00
  OriGene Technologies, Inc.

CD273 / PDL2 (20-200, His-tag)

CD273 / PDL2 Human Purified > 90 % by SDS - PAGE E. coli
0.5 mg / €820.00
  OriGene Technologies GmbH

CD273 / PDL2 (20-200, His-tag)

CD273 / PDL2 Human Purified > 90 % by SDS - PAGE E. coli
0.1 mg / €320.00
  OriGene Technologies GmbH

CD273 / PDL2

CD273 / PDL2 Human Human HEK293T cells
  Abnova Taiwan Corp.

CD273 / PDL2

CD273 / PDL2 Human Human HEK293T cells
  Abnova Taiwan Corp.

CD273 / PDL2

CD273 / PDL2 Human
  Abnova Taiwan Corp.

CD273 / PDL2

CD273 / PDL2 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

CD273 / PDL2

CD273 / PDL2 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

Positive controls for CD273 / PDL2 (1 products)

Catalog No. Species Pres. Purity   Source  

PDCD1LG2 overexpression lysate

PDCD1LG2 overexpression lysate
0.1 mg / €295.00
  OriGene Technologies, Inc.
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