AM05613FC-N CD274 / PDL1 antibody

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0.1 mg / €400.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

Quick Overview

Mouse anti Human CD274 / PDL1 MIH2

Product Description for CD274 / PDL1

Mouse anti Human CD274 / PDL1 MIH2.
Presentation: FITC
Product is tested for Flow Cytometry.

Properties for CD274 / PDL1

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms B7 homolog 1, B7H1, PD-L1, PDCD1 ligand 1, PDCD1L1, PDCD1LG1, Programmed cell death 1 ligand 1, Programmed death ligand 1
Presentation FITC
Reactivity Hu
Applications F
Clonality Monoclonal
Clone MIH2
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
Q9NZQ7
Immunogen:
Human CD274 transfected P815 cells.
GeneID:
29126
Remarks:
Cells from immunised mice were fused with cells of the P3U1 myeloma cell line.
Isotype control SM10F (for use in human samples)
Application Flow Cytometry: Use 10 µl of neat-1/10 diluted antibody to label 1x10e6 cells in 100 µl.
Background CD274 is reported to possess dual functions; inhibition of activated effector T cells and co-stimulation of native T cells. CD274 inhibits proliferation of activated T cells via ligation to the co-inhibitory molecule CD279 (programmed death-1; PD-1) leading to the secretion of the regulatory cytokine interleukin-10. CD274 has also been shown to costimulate early T cell priming and differentiation.
Deregulated CD274 function has been reported in chronic viral and intracellular bacterial infection, as well as in many autoimmune diseases and cancers.
Concentration 0.1 mg/ml
General Readings
  1. Kanai T, Totsuka T, Uraushihara K, Makita S, Nakamura T, Koganei K, et al. Blockade of B7-H1 suppresses the development of chronic intestinal inflammation. J Immunol. 2003 Oct 15;171(8):4156-63. PubMed PMID: 14530338.
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
This product is photosensitive and should be pretected from light.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Label:
Fluorescein Isothiocyanate Isomer 1
FITC
Purification:
Affinity Chromatography on Protein G
Buffer System:
PBS containing 0.09% Sodium Azide as preservative and 1% BSA as stabilizer.
Purity:
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
State:
Liqudi purified IgG fraction
Species Reactivity
Species reactivity (tested):
Human.
Specificity
Specificity:
This antibody detects CD274, also known as B7-H1 and PD-1L, a cell surface glycoprotein which is a member of the B7 family of co-stimulatory molecules. CD274 is expressed constitutively on macrophages and dendritic cells, and is induced on activated T-cells, B-cells, endothelial cells and epithelial cells in response to Interferons alpha, beta and gamma.
Gene ID 29126
FocusOn and Reviews
FocusOns:

Accessory Products

Proteins and/or Positive Controls

Proteins for CD274 / PDL1 (15 products)

Catalog No. Species Pres. Purity   Source  

CD274 / PDL1 (C-term DDK/His tag, transcript variant 1)

CD274 / PDL1 Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €748.00
  OriGene Technologies, Inc.

CD274 / PDL1 (C-term Fc tag)

CD274 / PDL1 Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €748.00
  OriGene Technologies, Inc.

CD274 / PDL1 (C-term DDK tag)

CD274 / PDL1 Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
SF9 cells
20 µg / €411.00
  OriGene Technologies, Inc.

CD274 / PDL1 (C-term DDK tag, transcript variant 1)

CD274 / PDL1 Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
SF9 cells
20 µg / €411.00
  OriGene Technologies, Inc.

CD274 / PDL1

CD274 / PDL1 Human > 95 %
Preparation: .
Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
10 µg / €299.00
  OriGene Technologies, Inc.

CD274 / PDL1 (Fc portion)

CD274 / PDL1 Human Purified > 95 % pure by SDS-PAGE and HPLC analyses CHO cells
0.1 mg / €250.00
  OriGene Technologies GmbH

CD274 / PDL1 (Fc portion)

CD274 / PDL1 Human Purified > 95 % pure by SDS-PAGE and HPLC analyses CHO cells
20 µg / €140.00
  OriGene Technologies GmbH

CD274 / PDL1 (19-238, His-tag)

CD274 / PDL1 Human Purified > 90 % by SDS - PAGE E. coli
0.1 mg / €820.00
  OriGene Technologies GmbH

CD274 / PDL1 (19-238, His-tag)

CD274 / PDL1 Human Purified > 90 % by SDS - PAGE E. coli
20 µg / €320.00
  OriGene Technologies GmbH

CD274 / PDL1

CD274 / PDL1 Human Human HEK293T cells
  Abnova Taiwan Corp.

CD274 / PDL1

CD274 / PDL1 Human
  Abnova Taiwan Corp.

CD274 / PDL1

CD274 / PDL1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

CD274 / PDL1

CD274 / PDL1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

CD274 / PDL1

CD274 / PDL1 Human
  Abnova Taiwan Corp.

CD274 / PDL1

CD274 / PDL1 Human
  Abnova Taiwan Corp.

Positive controls for CD274 / PDL1 (1 products)

Catalog No. Species Pres. Purity   Source  

CD274 overexpression lysate

CD274 overexpression lysate
0.1 mg / €295.00
  OriGene Technologies, Inc.
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