DM3036 CD3 antibody

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Mouse anti Human CD3 33.2A3


Product Description for CD3

Mouse anti Human CD3 33.2A3.
Presentation: Purified
Product is tested for Frozen Sections, Western blot / Immunoblot, Flow Cytometry, Paraffin Sections.

Properties for CD3

Product Category Primary Antibodies
Quantity 0.2 mg
Synonyms CD247, CD3 delta chain, CD3 epsilon chain, CD3 gamma chain, CD3 zeta chain, CD3D, CD3E, CD3G, CD3Z, T-cell surface glycoprotein CD3, T3D, T3G, T3Z, TCRZ
Presentation Purified
Reactivity Hu
Applications C, F, P, WB
Clonality Monoclonal
Clone 33.2A3
Host Mouse
Isotype IgG2a
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Biomeda

Datasheet Extract

Human leucocytes

Format: This antibody is supplied as liquid, purified immunoglobulin fraction in 50 mM Sodium Borate, 150 mM Sodium Chloride, 20% Glycerol and 0.05% Sodium azide, pH 8.0.
Isotype control AM03096PU-N
Application Immunofluorescence (5-20 µg/ml). Immunohistochemistry on frozen and formalin-fixed paraffin-embedded tissue sections (5-20 µg/ml using avidin-biotin system; An antigen demasking procedure is recommended when using formalin-fixed paraffin-embedded tissue sections). Flow cytometry (1-5 µg/106 cells). Western Blotting (1-5 µg/ml). Recommended positive control: Tonsil, Thymus, Spleen. Other applications not tested. Optimal dilutions of this antibody are dependent on conditions and should be determined by the user.
Concentration 0.4 mg/ml
General Readings
  1. Alberola-Ila J, Places L, de la Calle O, Romero M, Yagüe J, Gallart T, et al. Stimulation through the TCR/CD3 complex up-regulates the CD2 surface expression on human T lymphocytes. J Immunol. 1991 Feb 15;146(4):1085-92. PubMed PMID: 1671400.
  2. McMicvhael, A.J. etal, Leucocyte Typing III, Oxford University Press, 1987.
  3. Knapp, W. etal, Leucocyte Typing IV, Oxford Un
Storage Store the antibody at 4°C. Do not freeze! Shelf life: one year from despatch.

Aliquoting Instructions: Do not dilute the entire reconstituted solution at once. Withdraw aliquots as needed with a micropipette and keep concentrated stock at 4C. Dilute according to the particular application being used. In general, the 0.05M borate pH 8.0 containing 0.15M sodium chloride, 0.02% sodium azide, is a good dilutent to use with most antibodies. Avoid diluting the entire contents of the vial at once since the diluted solution may have reduced stability.
This antibody reacts with the human CD3 antigen of the TCR/CD3 complex. Other species not tested. This MAb is clustered in the 6th International Leucocytes Workshop on human leucocyte differentiation antigens in Kobe, Japan.

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