NB100-63677 CD3 antibody

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Mouse anti Rat CD3 1F4


Product Description for CD3

Mouse anti Rat CD3 1F4.
Presentation: FITC
Product is tested for Flow Cytometry.

Properties for CD3

Product Category Primary Antibodies
Quantity 0.05 mg
Synonyms CD247, CD3 delta chain, CD3 epsilon chain, CD3 gamma chain, CD3 zeta chain, CD3D, CD3E, CD3G, CD3Z, T-cell surface glycoprotein CD3, T3D, T3G, T3Z, TCRZ
Presentation FITC
Reactivity Rt
Applications F
Clonality Monoclonal
Clone 1F4
Host Mouse
Isotype IgM
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

F344 rat T cells stimulated with PMA (TPA) and calcium ionophore
Isotype control SM13F
Application flow cytometry / FACS analysis
Background T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits: CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.
Concentration 0.5 mg/ml
General Readings
  1. Nicolls, M.G. et al. (1992) Induction of long term specific tolerance to allografts in vivo by therapy with a monoclonal antibody to rat CD3. Transplantation 55: 459-468.
  2. Tanaka, T. et al. (1989) Characterization of a CD3-like rat T cells surface antigen recognised by a monoclonal antibody. J. Immunol. 142: 2791-2795.
Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer System:
Phosphate buffered saline, pH7.4, 1% Bovine Serum Albumin
Rat. Not yet tested in other species.

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