discontinued

SM1057A CD3 antibody

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1 mg / €740.00

Quick Overview

Mouse anti Human, Primate CD3 UCHT-1

SM1057A

Product Description for CD3

Mouse anti Human, Primate CD3 UCHT-1.
Presentation: Azide Free
Product is tested for Frozen Sections, Enzyme Immunoassay, Functional assay, Immunoprecipitation, Flow Cytometry.

Properties for CD3

Product Category Primary Antibodies
Quantity 1 mg
Synonyms CD247, CD3 delta chain, CD3 epsilon chain, CD3 gamma chain, CD3 zeta chain, CD3D, CD3E, CD3G, CD3Z, T-cell surface glycoprotein CD3, T3D, T3G, T3Z, TCRZ
Presentation Azide Free
Reactivity Hu, Prim
Applications C, E, F, FN, IP
Clonality Monoclonal
Clone UCHT-1
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Human infant thymocytes and lymphocytes from a patient with Sezary Syndrome.
Isotype control AM33027AF-N
Application Flow cytometry (Use 10ul of a 1/50-1/100 dilution to label 100 µl of whole blood). Immunohistochemistry on frozen sections.
Concentration 1 mg/ml
General Readings
  1. Beverley, P.C.L. and Callard, R.E. (1981). Distinctive functional characteristics of human T lymphocytes defined by E rosetting or a monoclonal anti T-cell antibody. Eur. J. Immunol. 11: 329-34.
  2. Kung P, Goldstein G, Reinherz EL, Schlossman SF. Monoclonal antibodies defining distinctive human T cell surface antigens. Science. 1979 Oct 19;206(4416):347-9. PubMed PMID: 314668.
  3. Clevers H, Dunlap S, Terhorst C. The transmembrane orientation of the epsilon chain of the TcR/CD3 complex. Eur J Immunol. 1988 May;18(5):705-10. PubMed PMID: 2967760.
  4. Clevers H, Alarcon B, Wileman T, Terhorst C. The T cell receptor/CD3 complex: a dynamic protein ensemble. Annu Rev Immunol. 1988;6:629-62. PubMed PMID: 3289580.
  5. Meuer, S.C., Acuto, O., Hussey, R.E., et al. (1983). Evidence for the T3-associated 90 k heterodimer as the T cell antigen receptor. Nature 303: 808-10.
  6. Clark EA, Ledbetter JA. Leukocyte cell surface enzymology: CD45 (LCA, T200) is a protein tyrosine phosphatase. Immunol Today. 1989 Jul;10(7):225-8. PubMed PMID: 2553046.
  7. Campana D, Thompson JS, Amlot P, Brown S, Janossy G. The cytoplasmic expression of CD3 antigens in normal and malignant cells of the T lymphoid lineage. J Immunol. 1987 Jan 15;138(2):648-55. PubMed PMID: 3098852.
  8. Erber, W.N., Pinching, A.J., Mason, D.Y. (1984). Immunocytochemical detection of T cell and B cell populations in routine blood smears. Lancet i: 1042-5.
  9. Erber WN, Mynheer LC, Mason DY. APAAP labelling of blood and bone-marrow samples for phenotyping leukaemia. Lancet. 1986 Apr 5;1(8484):761-5. PubMed PMID: 2870268.
  10. Denning, S.M., Tuck, D.T., Singer, K.H., Haynes, B.F. (1987). Activation of human thymocytes via CD3 and CD2 molecules. In McMichael, A.J. et al. Leucocyte Typing III: White Cell Differentiation Antigens. Edited by Oxford University Press pp 144-7.
  11. Grogan TM, Fielder K, Rangel C, Jolley CJ, Wirt DP, Hicks MJ, et al. Peripheral T-cell lymphoma: aggressive disease with heterogeneous immunotypes. Am J Clin Pathol. 1985 Mar;83(3):279-88. PubMed PMID: 2983525.
  12. Clark, E.A. et al. (1983). Evolution of epitopes on human and non-human primate lymphoid cell surface antigens. Immunogenetics 18: 599-615.
  13. Maggiorella MT, Monardo F, Koanga-Mogtomo ML, Cioè L, Sernicola L, Corrias F, et al. Detection of infectious simian immunodeficiency virus in B- and T-cell lymphomas of experimentally infected macaques. Blood. 1998 May 1;91(9):3103-11. PubMed PMID: 9558363.
Storage Store the antibody at -20°C. Avoid repeated freezing and thawing. Shelf live: 12 month from despatch
Format
State:
Azide Free
Specificity
Specificity:
UCH-T1 recognises a surface protein of peripheral blood T-cells of 19 kDa molecular weight. As a pan T-cell marker this antibody is present on more than 95% of circulation human peripheral T cells. This antibody stimulates T-cell proliferation in vitro. This clone is reported to cross react with chimpanzee (12) and Cynomolgus monkey (13).

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