AM06371SU-N CD309 / VEGFR-2 / Flk-1 antibody

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0.1 ml / €400.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

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Mouse anti Human CD309 / VEGFR-2 / Flk-1 4B4

AM06371SU-N

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  • AM06371SU-N

Product Description for CD309 / VEGFR-2 / Flk-1

Mouse anti Human CD309 / VEGFR-2 / Flk-1 4B4.
Presentation: Ascites
Product is tested for Flow Cytometry, Enzyme Immunoassay, Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot.

Properties for CD309 / VEGFR-2 / Flk-1

Product Category Primary Antibodies
Target Category
Quantity 0.1 ml
Synonyms FLK1, KDR, Kinase NYK, Kinase insert domain receptor, Protein-tyrosine kinase receptor Flk-1, VEGF Receptor 2, VEGFR2, Vascular endothelial growth factor receptor 2
Presentation Ascites
Reactivity Hu
Applications E, F, ICC/IF, WB
Clonality Monoclonal
Clone 4B4
Host Mouse
Isotype IgG1
Molecular weight 152 kDa
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P35968
Immunogen:
Purified recombinant extracellular fragment of human VEGFR-2 / KDR (aa20-764) fused with hIgGFc tag expressed in HEK293 cells.
GeneID:
3791
Application Western blot: 1/500-1/2000.
Immunofluorescence: 1/200-1/1000.
Flow Cytometry: 1/200-1/400.
ELISA: 1/10000.
Background KDR has also been designated as VEGFR-2 (Vascular endothelial growth factor receptor 2), CD309 (cluster of differentiation 309) and Flk-1 (fetal liver kinase 1). Vascular endothelial growth factor (VEGF) is a major growth factor for endothelial cells. KDR is one of the two receptors of the VEGF. This receptor, known as kinase insert domain receptor, is a type III receptor tyrosine kinase. It functions as the main mediator of VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis and sprouting. The signalling and trafficking of this receptor are regulated by multiple factors, including Rab GTPase, P2Y purine nucleotide receptor, integrin alphaVbeta3, T-cell protein tyrosine phosphatase, etc.. Mutations of this gene are implicated in infantile capillary hemangiomas.
General Readings
  1. Blood. 2004 Aug 1;104(3):788-94.
  2. FEBS Lett. 2002 Feb 13;512(1-3):107-10.
  3. EMBO J. 2001 Jun 1;20(11):2768-78.
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
State:
Ascitic fluid containing 0.03% Sodium Azide
Ascites
Specificity
Specificity:
This antibody recognizes Human VEGFR-2 / KDR. 
Other species not tested.
Gene ID 3791
FocusOn and Reviews
FocusOns:

Accessory Products

Proteins and/or Positive Controls

Proteins for CD309 / VEGFR-2 / Flk-1 (13 products)

Catalog No. Species Pres. Purity   Source  

CD309 / VEGFR-2 / Flk-1 (C-term DDK tag)

CD309 / VEGFR-2 / Flk-1 Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
SF9 cells
20 µg / €399.00
  OriGene Technologies, Inc.

CD309 / VEGFR-2 / Flk-1 (C-term DDK tag)

CD309 / VEGFR-2 / Flk-1 Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
SF9 cells
20 µg / €399.00
  OriGene Technologies, Inc.

CD309 / VEGFR-2 / Flk-1 (esKDR)

Inhibition of the VEGF165-induced proliferation of HUVE cells by recombinant human and mouse endogenous sKDR and sFlk-1 HUVECs were stimulated with 10 ng/ml VEGF165, both soluble receptors were added with a 100X excess. Human Purified > 95 % by SDS-PAGE and silver stain Insect cells
20 µg / €460.00
  OriGene Technologies GmbH

CD309 / VEGFR-2 / Flk-1 (esKDR)

Inhibition of the VEGF165-induced proliferation of HUVE cells by recombinant human and mouse endogenous sKDR and sFlk-1 HUVECs were stimulated with 10 ng/ml VEGF165, both soluble receptors were added with a 100X excess. Human Purified > 95 % by SDS-PAGE and silver stain Insect cells
5 µg / €240.00
  OriGene Technologies GmbH

CD309 / VEGFR-2 / Flk-1 (D1-D7)

SDS-PAGE analysis of recombinant human soluble KDR(D7) and sKDR(D7)-Fc derived from insect cells. Samples were loaded in 10% SDS-polyacrylamide gel under reducing and non-reducing conditions and stained with Silver stain. sKDR (D7)-Fc is able to form dimers whereas sKDR (D7) is not. Human Purified > 95 % pure as by SDS-PAGE and silver stain. Insect cells
5 µg / €150.00
  OriGene Technologies GmbH

CD309 / VEGFR-2 / Flk-1 (D1-D7)

SDS-PAGE analysis of recombinant human soluble KDR(D7) and sKDR(D7)-Fc derived from insect cells. Samples were loaded in 10% SDS-polyacrylamide gel under reducing and non-reducing conditions and stained with Silver stain. sKDR (D7)-Fc is able to form dimers whereas sKDR (D7) is not. Human Purified > 95 % pure as by SDS-PAGE and silver stain. Insect cells
50 µg / €340.00
  OriGene Technologies GmbH

CD309 / VEGFR-2 / Flk-1 (D1-7) (Fc Chimera)

SDS-PAGE analysis of recombinant human soluble KDR(D7) and sKDR(D7)-Fc derived from insect cells. Samples were loaded in 10% SDS polyacrylamide gel under reducing and non-reducing conditions and stained with Silver stain. As you can see sKDR (D7)-Fc is able to form dimers whereas sKDR (D7) is not. Human Purified > 90 % by SDS-PAGE and visualised by silver stain. Insect cells
50 µg / €290.00
  OriGene Technologies GmbH

CD309 / VEGFR-2 / Flk-1 (D1-7) (Fc Chimera)

SDS-PAGE analysis of recombinant human soluble KDR(D7) and sKDR(D7)-Fc derived from insect cells. Samples were loaded in 10% SDS polyacrylamide gel under reducing and non-reducing conditions and stained with Silver stain. As you can see sKDR (D7)-Fc is able to form dimers whereas sKDR (D7) is not. Human Purified > 90 % by SDS-PAGE and visualised by silver stain. Insect cells
10 µg / €180.00
  OriGene Technologies GmbH

CD309 / VEGFR-2 / Flk-1

CD309 / VEGFR-2 / Flk-1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

CD309 / VEGFR-2 / Flk-1

CD309 / VEGFR-2 / Flk-1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

CD309 / VEGFR-2 / Flk-1

CD309 / VEGFR-2 / Flk-1 Human
  Abnova Taiwan Corp.

CD309 / VEGFR-2 / Flk-1

CD309 / VEGFR-2 / Flk-1 Human
  Abnova Taiwan Corp.

CD309 / VEGFR-2 / Flk-1

CD309 / VEGFR-2 / Flk-1 Human
  Abnova Taiwan Corp.

Positive controls for CD309 / VEGFR-2 / Flk-1 (2 products)

Catalog No. Species Pres. Purity   Source  

VEGF Receptor 2 Lysate

Western Blot: VEGFR1/VEGFR2 Antibody [NB100-527] - Western Blot on VEGFR2 overexpression lysates.
  Novus Biologicals Inc.

VEGF Receptor 2 Lysate

Western Blot: VEGF Receptor 2 Lysate [NBL1-12223] - Western Blot experiments.  Left-Control; Right -Over-expression Lysate for KDR.
  Novus Biologicals Inc.
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