SM272F CD31 / PECAM1 antibody

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0.1 mg / €430.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

Quick Overview

Mouse anti Rhesus Monkey, Rat CD31 / PECAM1 TLD-3A12

SM272F

Product Description for CD31 / PECAM1

Mouse anti Rhesus Monkey, Rat CD31 / PECAM1 TLD-3A12.
Presentation: FITC
Product is tested for Flow Cytometry.

Properties for CD31 / PECAM1

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms EndoCAM, GPIIA', PECAM-1, Platelet endothelial cell adhesion molecule
Presentation FITC
Reactivity Rhesus Mky, Rt
Applications F
Clonality Monoclonal
Clone TLD-3A12
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
Q3SWT0
Immunogen:
Activated, Lewis rat derived microglial cells.
Spleen cells from immunised BALB/c mouse were fused with cells of the mouse SP2 myeloma cell line.
GeneID:
29583
Isotype control SM20F (for use in rat samples)
Application Flow Cytometry: Use 10 µl of neat antibody to label 10e6 cells in 100 µl.
Background CD31, also known as platelet endothelial cell adhesion molecule 1 (PECAM1), is a type I integral membrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is constitutively expressed on the surface of endothelial cells, and concentrated at the junction between them. It is also weakly expressed on many peripheral lymphoid cells and platelets.
CD31 has been used to measure angiogenesis in association with tumor recurrence. Other studies have also indicated that CD31 and CD34 can be used as markers for myeloid progenitor cells and recognize different subsets of myeloid leukemia infiltrates (granular sarcomas).
Concentration 0.1 mg/ml
General Readings
  1. Williams KC, Zhao RW, Ueno K, Hickey WF. PECAM-1 (CD31) expression in the central nervous system and its role in experimental allergic encephalomyelitis in the rat. J Neurosci Res. 1996 Sep 15;45(6):747-57. PubMed PMID: 8892086.
  2. Nakao A, Kimizuka K, Stolz DB, Neto JS, Kaizu T, Choi AM, et al. Carbon monoxide inhalation protects rat intestinal grafts from ischemia/reperfusion injury. Am J Pathol. 2003 Oct;163(4):1587-98. PubMed PMID: 14507665. (Free PMC Article available, 12 images available)
  3. Stevenson KS, McGlynn L, Hodge M, McLinden H, George WD, Davies RW, et al. Isolation, characterization, and differentiation of thy1.1-sorted pancreatic adult progenitor cell populations. Stem Cells Dev. 2009 Dec;18(10):1389-98. doi: 10.1089/scd.2008.0301. PubMed PMID: 19326970.
  4. Ott I, Keller U, Knoedler M, Götze KS, Doss K, Fischer P, et al. Endothelial-like cells expanded from CD34+ blood cells improve left ventricular function after experimental myocardial infarction. FASEB J. 2005 Jun;19(8):992-4. Epub 2005 Apr 6. PubMed PMID: 15814609.
  5. Fujimoto KL, Tobita K, Merryman WD, Guan J, Momoi N, Stolz DB, et al. An elastic, biodegradable cardiac patch induces contractile smooth muscle and improves cardiac remodeling and function in subacute myocardial infarction. J Am Coll Cardiol. 2007 Jun 12;49(23):2292-300. Epub 2007 May 25. PubMed PMID: 17560295. (Free PMC Article available, 6 images available)
  6. Thebault, P. et al. (2010) The C-type lectin-like receptor CLEC-1, expressed by myeloid cells and endothelial cells, is up-regulated by immunoregulatory mediators and moderates T cell activation. J Immunol. 183: 3099-108.
  7. Graham MJ, Crooke ST, Monteith DK, Cooper SR, Lemonidis KM, Stecker KK, et al. In vivo distribution and metabolism of a phosphorothioate oligonucleotide within rat liver after intravenous administration. J Pharmacol Exp Ther. 1998 Jul;286(1):447-58. PubMed PMID: 9655890.
  8. Haywood L, McWilliams DF, Pearson CI, Gill SE, Ganesan A, Wilson D, et al. Inflammation and angiogenesis in osteoarthritis. Arthritis Rheum. 2003 Aug;48(8):2173-7. PubMed PMID: 12905470.
  9. Kielian, T. and Hickey, W.F. (2010) Proinflammatory cytokine, chemokine, and cellular adhesion molecule expression during the acute phase of experimental brain abscess development. Am J Pathol. 157: 647-58.
  10. Lochhead JJ, McCaffrey G, Quigley CE, Finch J, DeMarco KM, Nametz N, et al. Oxidative stress increases blood-brain barrier permeability and induces alterations in occludin during hypoxia-reoxygenation. J Cereb Blood Flow Metab. 2010 Sep;30(9):1625-36. doi: 10.1038/jcbfm.2010.29. Epub 2010 Mar 17. PubMed PMID: 20234382. (Free PMC Article available, 5 images available)
  11. Arkudas A, Tjiawi J, Bleiziffer O, Grabinger L, Polykandriotis E, Beier JP, et al. Fibrin gel-immobilized VEGF and bFGF efficiently stimulate angiogenesis in the AV loop model. Mol Med. 2007 Sep-Oct;13(9-10):480-7. PubMed PMID: 17762899. (Free PMC Article available, 8 images available)
  12. Nakao A, Huang CS, Stolz DB, Wang Y, Franks JM, Tochigi N, et al. Ex vivo carbon monoxide delivery inhibits intimal hyperplasia in arterialized vein grafts. Cardiovasc Res. 2011 Feb 1;89(2):457-63. doi: 10.1093/cvr/cvq298. Epub 2010 Sep 16. PubMed PMID: 20851811. (Free PMC Article available, 6 images available)
  13. Ohnishi T, Arnold LL, Clark NM, Wisecarver JL, Cohen SM. Comparison of endothelial cell proliferation in normal liver and adipose tissue in B6C3F1 mice, F344 rats, and humans. Toxicol Pathol. 2007 Dec;35(7):904-9. PubMed PMID: 18098037.
  14. Schilte MN, Loureiro J, Keuning ED, ter Wee PM, Celie JW, Beelen RH, et al. Long-term intervention with heparins in a rat model of peritoneal dialysis. Perit Dial Int. 2009 Jan-Feb;29(1):26-35. PubMed PMID: 19164250.
  15. Seegers HC, Hood VC, Kidd BL, Cruwys SC, Walsh DA. Enhancement of angiogenesis by endogenous substance P release and neurokinin-1 receptors during neurogenic inflammation. J Pharmacol Exp Ther. 2003 Jul;306(1):8-12. Epub 2003 Mar 26. PubMed PMID: 12660314.
  16. Wilson E, Jackson S, Cruwys S, Kerry P. An evaluation of the immunohistochemistry benefits of boric acid antigen retrieval on rat decalcified joint tissues. J Immunol Methods. 2007 Apr 30;322(1-2):137-42. Epub 2007 Feb 23. PubMed PMID: 17362980.
  17. Willis CL, Meske DS, Davis TP. Protein kinase C activation modulates reversible increase in cortical blood-brain barrier permeability and tight junction protein expression during hypoxia and posthypoxic reoxygenation. J Cereb Blood Flow Metab. 2010 Nov;30(11):1847-59. doi: 10.1038/jcbfm.2010.119. Epub 2010 Aug 11. PubMed PMID: 20700133. (Free PMC Article available, 5 images available)
  18. Salehi-Had H, Roh MI, Giani A, Hisatomi T, Nakao S, Kim IK, et al. Utilizing targeted gene therapy with nanoparticles binding alpha v beta 3 for imaging and treating choroidal neovascularization. PLoS One. 2011 Apr 29;6(4):e18864. doi: 10.1371/journal.pone.0018864. PubMed PMID: 21559527. (Free PMC Article available, 6 images available)
  19. MacLean AG, Orandle MS, Alvarez X, Williams KC, Lackner AA. Rhesus macaque brain microvessel endothelial cells behave in a manner phenotypically distinct from umbilical vein endothelial cells. J Neuroimmunol. 2001 Aug 30;118(2):223-32. PubMed PMID: 11498257.
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Label:
Fluorescein Isothiocyanate Isomer I
FITC
Purification:
Affinity Chromatography on Protein G
Buffer System:
PBS
Preservatives:
0.09% Sodium Azide
Stabilizers:
1% BSA
State:
Liquid purified IgG fraction from Tissue Culture Supernatant
Species Reactivity
Species reactivity (expected):
Rhesus Monkey.
Species reactivity (tested):
Rat.
Specificity
Specificity:
This antibody recognises PECAM-1 (CD31), a type 1 transmembrane protein expressed primarily on endothelial cells, platelets and leucocytes.
Clone TLD-3A12 has been shown to partially block the proliferative response of antigen-specific CD4+ T cells to antigen-presenting cells and relevant antigen (Stevenson, K.S. et al. 2009). We recommend SM272LE for use in functional studies.
This clone has been reported as suitable for use on formalin-fixed paraffin-embedded sections pre-treated with 0.2M boric acid, pH7.0. (Wilson et al. 2007). Mouse anti Rat CD31, clone TLD-3A12 has been shown to be cross-reactive with endothelial cells derived from rhesus macaque (Maclean et al. 2001).
Gene ID 29583

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