DM3054 CD32 / FcRII antibody

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Mouse anti Human CD32 / FcRII 7.30


Product Description for CD32 / FcRII

Mouse anti Human CD32 / FcRII 7.30.
Presentation: Purified
Product is tested for Frozen Sections, Immunocytochemistry/Immunofluorescence, Flow Cytometry, Paraffin Sections.

Properties for CD32 / FcRII

Product Category Primary Antibodies
Quantity 0.2 mg
Presentation Purified
Reactivity Hu
Applications C, F, ICC/IF, P
Clonality Monoclonal
Clone 7.30
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Biomeda

Datasheet Extract

K562 and FcgRII+L cells.
Isotype control AM03095PU-N, SM10P (for use in human samples)
Application Immunofluorescence: 5-20 µg/mL. Flow cytometry: 1-5 µg/106 cells. Immunohistochemistry: 5-20 µg/mL on frozen sections using avidin-biotin system. This antibody can be used on frozen cryostat sections as well as formalin-fixed, paraffin-embedded tissue sections. Prolonged fixation is buffered formalin can destroy the epitope. For consistent and reproducible results on formalin-fixed, paraffin-embedded sections, a high temperature antigen unmasking technique is required.
This antibody was quality controlled in flow cytometry.
Recommended positive control: Macrophages, Granulocytes, Eosinophils. Other applications not tested. Optimal dilutions of this antibody are dependent on conditions and should be determined by the user.
Concentration 0.4 mg/ml
General Readings
  1. Ierino, B., et.al., J. Immunol. 150: 1794-1803, 1993.
  2. Stuart, S.G., et.al., J. Exp. Med. 166: 1668, 1987.
  3. Hogg, N., Immun
Storage Store the antibody at 4°C. Do not freeze! Shelf life: one year from despatch.

Do not dilute the entire reconstituted solution at once. Withdraw aliquots as needed with a micropipette and keep concentrated stock at 4°C. Dilute according to the particular application being used. In general, the 0.05M borate pH 8.0 containing 0.15M sodium chloride, 0.02% sodium azide, is a good dilutent to use with most antibodies. Avoid diluting the entire contents of the vial at once since the diluted solution may have reduced stability.
This antibody reacts with a 40kD (39-48kD, Endo F:35 and 29kD) polymorphic transmembranous highly glycosylated glycoprotein, also known as Fc-gRII receptor, gp40.

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