AM00010PU-N CD324 / Cadherin-1 (incl. pos. control) antibody

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0.1 mg / €430.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

Quick Overview

Mouse anti Human CD324 / Cadherin-1 22F8

Product Description for CD324 / Cadherin-1

Mouse anti Human CD324 / Cadherin-1 22F8.
Properties: (incl. pos. control)
Presentation: Purified
Product is tested for Western blot / Immunoblot.

Properties for CD324 / Cadherin-1

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms CAM 120/80, CDH1, CDHE, E-cadherin, Epithelial cadherin, UVO, Uvomorulin
Presentation Purified
Reactivity Hu
Applications WB
Clonality Monoclonal
Clone 22F8
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P12830
Immunogen:
Peptide conjugated to hemocyanin.
Epitope: cytoplasmic domain.
GeneID:
999
Isotype control AM03095PU-N, SM10P (for use in human samples)
Add. information Cell lysate from untreated A431 cells as positive control is supplied with this product.
Application Immunoblotting: 0.5 μg/ml for HRPO/ECL detection.
Recommended buffer: Casein/Tween 20 based blocking and blot incubation buffer.
Use cell lysate from untreated A431 cells as positive control.
Background Cadherins are a family of transmembrane glycoproteins that play a key role in calcium-dependent cell-cell adhesion. Several members of the cadherin family have been identified so far, including E-(epithelial), P-(placental), N-(neuronal), and M- muscle) cadherin. E-cadherin is expressed
in most epithelial tissues.
Protocols Positive control provided: Control cell lysate A 431 untreated
Format: Lyophilized cell lysate from serum starved A431 cells.
Reconstitute by addition of 200 µl H2O. After complete solubilization add 200 µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min.
Applications: The positive control cell lysate is recommended for immunoblot applications.
20 ml of positive control cell lysate correspond to ca. 20.000 cells.
Use 20 µl / lane (mini gel) for HRPO/ECL detection of the target proteins.
Please note:
The lyophilized cell lysates contain SDS and are not recommended for applications with native proteins such as immunoprecipitation.
Storage: In aliquots at -20°C. Avoid repeated freezing and thawing.
Storage For long-term storage, freeze lyophilizate upon arrival (-20°C).
Upon reconstitution, aliquote and freeze in liquid nitrogen; reconstituted antibody can be stored frozen at -80°C up to 1 year. Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months. Avoid repeated freezing and thawing.
Format
Purification:
The antibody was purified from serum-free cell culture supernatant by subsequent ultrafiltration and size exclusion chromatography.
Buffer System:
2 x PBS / 0.09% sodium azide / PEG and sucrose
State:
Lyophilized Ig fraction
Purified
Reconstitution:
Restore with 1 ml H2O (15 min, RT).
Specificity
Specificity:
This antibody specifically recognizes E-cadherin at 124 kDa.
Species:
Human.
Gene ID 999
FocusOn and Reviews
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Accessory Products

Proteins and/or Positive Controls

Proteins for CD324 / Cadherin-1 (13 products)

Catalog No. Species Pres. Purity   Source  

CD324 / Cadherin-1

CD324 / Cadherin-1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

CD324 / Cadherin-1

CD324 / Cadherin-1 Human Purified E. coli
  Acris Antibodies GmbH

CD324 / Cadherin-1

CD324 / Cadherin-1 Human Purified E. coli
  Acris Antibodies GmbH

CD324 / Cadherin-1

CD324 / Cadherin-1 Human Purified E. coli
  Acris Antibodies GmbH

CD324 / Cadherin-1 (24-709, His-tag)

CD324 / Cadherin-1 Human Purified > 90 % by SDS – PAGE Insect cells
0.25 mg / €1,230.00
  Acris Antibodies GmbH

CD324 / Cadherin-1 (24-709, His-tag)

CD324 / Cadherin-1 Human Purified > 90 % by SDS – PAGE Insect cells
50 µg / €370.00
  Acris Antibodies GmbH

CD324 / Cadherin-1

CD324 / Cadherin-1 Human
  Abnova Taiwan Corp.

CD324 / Cadherin-1

CD324 / Cadherin-1 Human Purified
  Abnova Taiwan Corp.

CD324 / Cadherin-1

CD324 / Cadherin-1 Human Purified
  Abnova Taiwan Corp.

CD324 / Cadherin-1

CD324 / Cadherin-1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

CD324 / Cadherin-1

CD324 / Cadherin-1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

CD324 / Cadherin-1

CD324 / Cadherin-1 Human
  Abnova Taiwan Corp.

CD324 / Cadherin-1

CD324 / Cadherin-1 Human
  Abnova Taiwan Corp.

Positive controls for CD324 / Cadherin-1 (2 products)

Catalog No. Species Pres. Purity   Source  

CDH1 overexpression lysate

CDH1 overexpression lysate
0.1 mg / €480.00
  OriGene Technologies, Inc.

E Cadherin Lysate

Western Blot: E Cadherin Lysate [NBL1-09024] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for E-Cadherin
  Novus Biologicals Inc.
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